RESUMO
The intrinsic ruggedness of Enterococcus faecalis is responsible for its widespread distribution in nature and is often viewed as an important virulence determinant. Previously, we showed that the ClpB ATPase is negatively regulated by CtsR and is required for thermotolerance and virulence in a Galleria mellonella invertebrate model. Here, we used in silico, Northern blot and quantitative real-time PCR analyses to identify additional members of the CtsR regulon, namely the clpP peptidase and the clpC and clpE ATPases. When compared to the parent strain, virulence of the ΔctsR strain in G. mellonella was significantly attenuated.
Assuntos
Adenosina Trifosfatases/biossíntese , Proteínas de Bactérias/biossíntese , Enterococcus faecalis/genética , Enterococcus faecalis/patogenicidade , Proteínas de Choque Térmico/biossíntese , Lepidópteros/microbiologia , Proteínas Repressoras/biossíntese , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/metabolismo , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sequência de Bases , Endopeptidase Clp/biossíntese , Endopeptidase Clp/metabolismo , Enterococcus faecalis/metabolismo , Regulação Bacteriana da Expressão Gênica , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Resposta ao Choque Térmico/genética , Regiões Promotoras Genéticas , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Virulência , Fatores de Virulência/biossíntese , Fatores de Virulência/genéticaRESUMO
Here we report the presence and expression levels of the vanC1 and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC1 and vanC(2/3) genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.
Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/genética , Resistência a Vancomicina/genética , Vancomicina/farmacologia , Animais , Galinhas , Cloaca/microbiologia , DNA Bacteriano/análise , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Enterococcus faecalis/isolamento & purificação , Genes Bacterianos/genética , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Here we report the presence and expression levels of the vanC 1 and vanC 2/3 genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC 1 and vanC 2/3 genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC 1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.
