Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression.

Aim. The effects of cryopreservation on adipose tissue-derived mesenchymal stem cells are not clearly documented, as there is a growing body of evidence about the importance of adipose-derived mesenchymal stem cells for regenerative therapies. The aim of this study was to analyze human adipose tissue-derived mesenchymal stem cells phenotypic expression (CD34, CD45, CD73, CD90, CD105, and CD49d), colony forming unit ability, viability, and differentiation potential before and after cryopreservation. Materials and Methods. 12 samples of the adipose tissue were collected from a healthy donor using the liposuction technique. The cell isolation was performed by enzymatic digestion and then the cells were cultured up to passage 2. Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively. Results. The immunophenotypic markers expression was largely preserved, and their multipotency was maintained. However, after cryopreservation, the cells decreased α4-integrin expression (CD49d), cell viability, and number of colony forming units. Conclusions. These findings suggest that ADMSC transplanted after cryopreservation might compromise the retention of transplanted cells in the host tissue. Therefore, further studies are warranted to standardize protocols related to cryopreservation to attain full benefits of stem cell therapy.

Hiperparatireoidismo primário: experiência com 14 casos / Primary hyperparathyroidism: experience with 14 cases

Foram estudados retrospectivamente 14 pacientes (nove mulheres e cinco homens), com idade media de 51,9 anos, portadores de hiperparatireoidismo primario. Treze (92,8 por cento) apresentavam sintomatologia neuropsiquiatrica oumusculoesqueletica. Todos exibiam hipercalcemia (12,04 ñ 1,42 mg/ por cento, hipercalciuria (9,62 ñ 1,32 mg/ por cento) e hipofosfatemia (2,09 ñ 0,35 mg/ por cento). Dez pacientes (71,4 por cento) apresentavam complicacoes metabolicas do hiperparatireoidismo primario, sendo as mais comuns: ulcera peptica e nefrocalcinose. O adenoma de paratireoide (92,2 por cento) foi a causa mais comum. Realizou-se a exerese de apenas uma glandula em seis pacientes (42,8 por cento), duas glandulas em seis (42,8 po cento) e de tres glandulas e meia aratireoidectomia subtotal) em dois (14,3 por cento). A mortalidade operatoria foi nula e o hipoparatireoidismo pos-operatorio transitorio (21,3 por cento) foi a unica complicacao.

Avaliaçäo da duraçäo da anti-sepsia pré-operatória da pele abdominal / Evaluation of the duration of preopoerative antisepsis of the abdominal skin

Foi avaliada a eficácia da anti-sepsia da pele abdominal por diferentes períodos de tempo. Um total de 64 pacientes foram submetidos a anti-sepsia da pele com povidina-iodo por 3, 5, 10 minutos, e por dois períodos de 5 minutos cada. Amostras para estudos bacteriológicos para bactérias aeróbica foram obtidas imediatamente antes, 5 minutos, 1 hora e 3 horas após a desinfecçäo. A reduçäo na populaçäo bacteriana foi acima de 96% em todos os grupos e näo houve diferença estatisticamente significativa entre os diferentes grupos, 5 minutos, 1 hora e 3 horas após a degermaçäo da pele