Regeneration of Capto Core 700 resin through high throughput and laboratory scale studies and impact on production of a SARS-CoV-2 vaccine candidate.

During the development of a SARS-CoV-2 vaccine candidate, at the height of the COVID-19 pandemic, raw materials shortages, including chromatography resins, necessitated the determination of a cleaning in place (CIP) strategy for a multimodal core-shell resin both rapidly and efficiently. Here, the deployment of high throughput (HT) techniques to screen CIP conditions for cleaning Capto Core 700 resin exposed to clarified cell culture harvest (CCCH) of a SARS-CoV-2 vaccine candidate produced in Vero adherent cell culture are described. The best performing conditions, comprised of 30% n-propanol and ≥0.75 N NaOH, were deployed in cycling experiments, completed with miniature chromatography columns, to demonstrate their effectiveness. The success of the CIP strategy was ultimately verified at the laboratory scale. Here, its impact was assessed across the entire purification process which also included an ultrafiltration/diafiltration step. It is shown that the implementation of the CIP strategy enabled the re-use of the Capto Core 700 resin for up to 10 cycles without any negative impact on the purified product. Hence, the strategic combination of HT and laboratory-scale experiments can lead rapidly to robust CIP procedures, even for a challenging to clean resin, and thus help to overcome supply shortages.

Cross-Sectional Survey of Graduate Clinical Nutrition Students Describing Dietary Choices and Recommendations.

Objectives: Little data exists describing the dietary habits of nutrition students, including whether students' personal dietary choices influence their clinical recommendations. Study objectives were to: (1) Identify common dietary choices made by nutrition students, (2) determine students' rationale for choosing or avoiding specific diets, (3) identify key factors used to make dietary recommendations.Methods: An anonymous online survey was distributed to graduate-level clinical nutrition students who were attending five American universities. Data collected included demographics, current diet, reasons for following the current diet, reasons for recommending diets, and degree completion status. Percentage of diets chosen and rationale for dietary decisions were compared. Diets chosen and recommended were stratified and compared by degree completion status via chi-square test.Results: 208 participants completed the survey. The top diets students reported following were Whole Foods/Unprocessed (49%) and Gluten-Free (16%), followed by Vegan/Vegetarian (13%) and Paleo (12.5%). The top reasons identified for choosing a personal diet were health optimization and food allergy/intolerance/sensitivity. The top factors used to make dietary recommendations were clients' preferences/resources and results of laboratory testing. A Whole Foods/Unprocessed diet was also selected by the largest proportion of students as one they would recommend to clients-97% of students early in their program and 98.3% of students late in their program would recommend it to a client.Conclusion: A Whole Foods/Unprocessed diet was the most followed diet and the one most likely to be recommended to future clients, irrespective of students' degree completion progress.