RESUMO
In this letter, we present experimental results of antibody detection using a biosensor based on photonic bandgap structures, which are interrogated using a power-based readout technique. This interrogation method allows a real-time monitoring of the association process between the antigen probes and the target antibodies, allowing the instantaneous observation of any interaction event between molecules. because etunable lasers and optical spectrum analyzers are avoided for the readout, a drastic reduction of the final cost of the platform is obtained. Furthermore, the performance of the biosensing system is significantly enhanced due to the large number of data values obtained per second.
Assuntos
Anticorpos/imunologia , Técnicas Biossensoriais/economia , Técnicas Biossensoriais/métodos , Fótons , Soroalbumina Bovina/imunologia , Animais , Técnicas Biossensoriais/instrumentação , Bovinos , Técnicas Analíticas Microfluídicas , Soroalbumina Bovina/análise , Fatores de TempoRESUMO
A technique for the development of low-cost and high-sensitivity photonic biosensing devices is proposed and experimentally demonstrated. In this technique, a photonic bandgap structure is used as transducer, but its readout is performed by simply using a broadband source, an optical filter, and a power meter, without the need of obtaining the transmission spectrum of the structure; thus, a really low-cost system and real-time results are achieved. Experimental results show that it is possible to detect very low refractive index variations, achieving a detection limit below 2×10(-6) refractive index units using this low-cost measuring technique.
Assuntos
Dispositivos Ópticos/economia , Fótons , Limite de Detecção , Cloreto de Sódio/análise , Fatores de TempoRESUMO
We report an experimental demonstration of single-strand DNA (ssDNA) detection at room temperature using a photonic-crystal-waveguide-based optical sensor. The sensor surface was previously biofunctionalized with ssDNA probes to be used as specific target receptors. Our experiments showed that it is possible to detect these hybridization events using planar photonic-crystal structures, reaching an estimated detection limit as low as 19.8 nM for the detection of the complementary DNA strand.
