Revisión sistemática de intervenciones de fisioterapia respiratoria y sus efectos en la función pulmonar en pacientes obesos intervenidos de cirugía bariátrica / Systematic review of Respiratory Physiotherapy interventions and their effects on lung function in obese patients operated of bariatric surgery

Introducción La incidencia de obesidad alcanza casi un tercio de la población mundial. En España habrá más de 27 millones de personas con obesidad para 2030, suponiendo una enorme carga socioeconómica, obligando a realizar intervenciones como la cirugía bariátrica (CB) que también tiene sus complicaciones. En los últimos años ha existido un avance de técnicas de fisioterapia respiratoria (FR) por ejemplo, empleando ejercicios respiratorios o utilizando presión positiva (PP). Objetivo Revisar sistemáticamente las intervenciones de FR en CB centrándonos en el tipo de intervención, momento y sus efectos sobre la función pulmonar. Metodología Se realizó la búsqueda en las bases PubMed, CINAHL y PEDro hasta marzo del 2022. Se incluyeron estudios de intervenciones de FR, que midieran la función pulmonar, en contexto pre-posquirúrgico de CB. Se excluyeron los trabajos que no aportaran datos originales, intervención no realizada por fisioterapeutas o que no miden función respiratoria. La calidad metodológica de los estudios se evaluó con la escala PEDro y JBI cheklist for cohort studies. Resultados Fueron incluidos 16 documentos, 20% presentó excelente calidad metodológica y 50% buena. El tamaño muestral varió entre 24 y 148, al igual que la edad (entre 18-60 años) incluso alcanzando 75 años en algún estudio, predominando el género femenino. El tipo de intervenciones (manuales, instrumentales o educacionales), momento de aplicación (pre y posquirúrgicas) y resultados evaluados fueron muy heterogéneos. Conclusiones Existe gran variabilidad de intervenciones de fisioterapia en CB, la mayoría (75%) mejoran la función pulmonar, otras indican falta de eficacia y algunas no indican cambios (AU)

Introduction The incidence of obesity reaches almost a third of the world population. In Spain, there will be more than 27 million people with obesity in 2030. Assuming an enormous socioeconomic burden, forcing interventions such as bariatric surgery, whit their complications. In recent years, there has been an advance in Respiratory Physiotherapy (RP) techniques, for example, using breathing exercises or using positive pressure (PP). Objective To systematically review RP interventions in bariatric surgery, focusing on the type of intervention, timing, and its effects on lung function. Methodology PubMed, CINAHL and PEDro databases were searched until March 2022. RP emergency studies were included, which measured lung function, in the pre-post-surgical context of bariatric surgery. Studies that did not provide original data, intervention not performed by physiotherapists or that did not measure respiratory function were excluded. The methodological quality of studies was evaluated with the PEDro scale and the JBI checklist for cohort studies. Results 16 documents were included, 20% presented excellent methodological quality and 50% good. The sample size varied between 24 and 148 and age (between 18-60 years), even reaching 75 years in some studies, with the female gender predominating. The type of outbreak (manual, instrumental or educational), time of application (pre and post-surgery) and results evaluated were very heterogeneous. Conclusions There is a high variability in physiotherapy interventions used in bariatric surgery, most (75%) improve lung function, others indicate lack of efficacy and some do not indicate changes (AU)

Activity of imipenem/relebactam against Enterobacterales and Pseudomonas aeruginosa in Spain. SMART 2016-2020.

OBJECTIVE: To determine susceptibility to the novel ß-lactam/ß-lactamase inhibitor combination imipenem/relebactam in clinical isolates recovered from intra-abdominal (IAI), urinary (UTI), respiratory (RTI) and bloodstream (BSI) infections in the SMART (Study for Monitoring Antimicrobial Resistance Trends) study in SPAIN during 2016 - 2020. METHODS: Broth microdilution MICs for imipenem/relebactam and comparators were determined by a central laboratory against isolates of Enterobacterales and Pseudomonas aeruginosa. MICs were interpreted using EUCAST-2021 breakpoints. RESULTS: In total, 5,210 Enterobacterales and 1,418 P. aeruginosa clinical isolates were analyzed. Imipenem/relebactam inhibited 98.8% of Enterobacterales. Distinguishing by source of infection susceptibility was 99.1% in BSI, 99.2% in IAI, 97.9% in RTI, and 99.2% in UTI. Of intensive care unit isolates (ICU) 97.4% were susceptible and of non-ICU isolates 99.2% were susceptible. In Enterobacterales, activity against Class A, Class B and Class D carbapenemases was 96.2%, 15.4% and 73.2%, respectively. In P. aeruginosa, imipenem/relebactam was active in 92.2% of isolates. By source of infection it was 94.8% in BSI, 92.9% in IAI, 91.7% in RTI, and 93.1% in UTI. An 88.7% of ICU isolates and 93.6% of non-ICU isolates were susceptible to imipenem/relebactam. Imipenem/relebactam remained active against P. aeruginosa ceftazidime-resistant (76.3%), cefepime-resistant (73.6%), imipenem-resistant (71.5%) and piperacillin-resistant (78.7%) isolates. Of all multidrug-resistant or difficult-to-treat resistance P. aeruginosa isolates, 75.1% and 46.2%, respectively, were susceptible to imipenem/relebactam. CONCLUSIONS: Imipenem/relebactam showed high rates of susceptibility in Enterobacterales and P. aeruginosa isolates from different sources of infection as well as depending on patients' location (ICU or non-ICU scenarios).

Tríada találgica. Una nueva entidad clínica a partir de una serie de casos / Thalalgic triad. A new clinical entity from a case series

Introducción: El dolor crónico en el talón es un cuadro frecuente en la patología del pie. Este tipo de cuadros no están causados por una sola lesión, sino que es el resultado de la combinación de una serie de cuadros clínicos, especialmente por una neuropatía de Baxter y una fascitis plantar. Desde hace varios años, y tras analizar una serie de casos clínicos, se observa que los pacientes con dolor crónico en el talón cursan con un proceso de fasciosis plantar, edema óseo de calcáneo o síndrome congestivo de calcáneo y neuropatía del nervio de Baxter. A raíz de este hallazgo concluimos en una nueva entidad clínica denominada tríada találgica, la cual no presenta referencias bibliográficas anteriores en la literatura científica. Pacientes y métodos: Se ha llevado a cabo una serie de casos de 10 pacientes a los que se les diagnostica una combinación de 3 patologías, denominada tríada találgica, que cursa con aparición de fasciosis plantar, edema óseo de calcáneo (congestión calcánea) y neuropatía compresiva de Baxter. Cada una de estas patologías se trata de forma independiente con distintas terapias, tales como soportes plantares, infiltraciones, tratamiento farmacológico o cirugía. Resultados: Un total de 10 mujeres con una edad media de 53 años fueron diagnosticadas de fasciosis plantar, edema óseo de calcáneo y neuropatía compresiva de Baxter mediante diferentes pruebas, como la ecografía. Estas pacientes fueron tratadas mediante infiltraciones de colágeno, extracción del líquido sanguinolento del calcáneo, terapia electrolisis percutánea intratisular, soportes plantares, tratamiento farmacológico y ondas de choque. El 40 % de la muestra se encontraba de baja laboral debido al dolor incapacitante de esta tríada y el 10 % en situación de jubilación. Conclusiones: La tríada találgica es una entidad clínica desconocida, con un diagnóstico complejo que combina pruebas complementarias con diagnóstico clínico...(AU)

Introduction: Chronic heel pain is a frequent condition in foot pathology. These types of pictures are not caused by a single injury, but are the result of a combination of a series of clinical pictures, especially Baxter's neuropathy and plantar fasciitis. For several years and after analyzing a series of clinical cases, it has been observed that patients with chronic pain in the heel present with a process of plantar fasciosis, calcaneal bone edema or congestive calcaneal syndrome and Baxter nerve neuropathy. As a result of this finding, we conclude on a new clinical entity called thatalgic triad, which does not present previous bibliographic references in the scientific literature. Patients and methods: A series of cases of 10 patients who were diagnosed with a combination of 3 pathologies, called the thatalgic triad, has been carried out, with the appearance of plantar fasciosis, calcaneal bone edema (calcaneal congestion) and neuropathy Baxter compression. Each of these pathologies is treated independently with different therapies, such as plantar supports, infiltrations, drug treatment or surgery. Results: A total of 10 women with a mean age of 53 years were diagnosed with plantar fasciosis, calcaneal bone edema and Baxter's compressive neuropathy by different tests, such as ultrasound. These patients were treated by collagen infiltrations, removal of bloody fluid from the calcaneus, intratissular percutaneous electrolysis therapy, plantar supports, pharmacological treatment, and shock waves. 40 % of the sample was on sick leave due to the disabling pain of this triad and 10 % were in retirement. Conclusions: The thatalgic triad is an unknown clinical entity with a complex diagnosis that combines complementary tests with clinical diagnosis. Likewise, there are very few bibliographic references on this subject.(AU)

Antimicrobial activity of ceftolozane-tazobactam against Enterobacterales and Pseudomonas aeruginosa recovered during the Study for Monitoring Antimicrobial Resistance Trends (SMART) program in Spain (2016-2018).

OBJECTIVE: To analyse the susceptibility to ceftolozane-tazobactam and comparators in Enterobacterales and Pseudomonas aeruginosa isolates recovered from intraabdominal (IAI), urinary (UTI), respiratory (RTI) and bloodstream infection (BSI) in the SMART (Study for Monitoring Antimicrobial Resistance Trends) study. METHODS: The susceptibility of 5,351 isolates collected in 11 Spanish hospitals (2016-2018) were analysed (EUCAST-2020 criteria) by broth microdilution and were phenotypically studied for the presence of extended-spectrum beta-lactamases (ESBL). Ceftolozane-tazobactam and/or carbapenem resistant isolates were genetically characterized for ESBL and carbapenemases. RESULTS: Escherichia coli was the most frequent pathogen (49.3% IAI, 54.9% UTI, 16.7% RTI and 50% BSI), followed by Klebsiella pneumoniae (11.9%, 19.1%, 13.1% and 15.4%, respectively). P. aeruginosa was isolated in 9.3%, 5.6%, 32% and 9%, respectively. The frequency of isolates with ESBLs (2016-2017) was: 30.5% K. pneumoniae, 8.6% E. coli, 2.3% Klebsiella oxytoca and 0.7% Proteus mirabilis. Ceftolozane-tazobactam was very active against non-ESBL-(99.3% susceptible) and ESBL-(95.2%) producing E. coli being less active against K. pneumoniae (98% and 43.1%, respectively) isolates. CTX-M-15 was the most prevalent ESBL in E. coli (27.5%) and K. pneumoniae (51.9%) frequently associated with OXA-48-like carbapenemase. Overall, 93% of P. aeruginosa isolates were susceptible to ceftolozane-tazobactam, preserving this activity (>75%) in isolates resistant to other beta-lactams except in those resistant to meropenen or ceftazidime-avibactam. GES-5, PER-1, VIM-1/2 were the most prevalent enzymes in isolates resistant to ceftolozane-tazobactam. CONCLUSIONS: Ceftolozane-tazobactam showed high activity rates against isolates recovered in the SMART study although it was affected in K. pneumoniae and P. aeruginosa isolates with ESBL and/or carbapenemases.

Monitoring the antimicrobial susceptibility of Gram-negative organisms involved in intraabdominal and urinary tract infections recovered during the SMART study (Spain, 2016 and 2017).

OBJECTIVE: Continuous antimicrobial resistance surveillance is recommended by Public Health authorities. We up-dated data from the SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study in Spain. METHODS: The antimicrobial susceptibility data and extended-spectrum beta-lactamase (ESBL) production in isolates recovered from intra-abdominal (IAI) (n=1,429) and urinary tract (UTI) (n=937) infections during the 2016- 2017 SMART study in 10 Spanish hospitals were analysed. RESULTS: Escherichia coli was the most frequently microorganism isolated (48.3% and 53.7%) followed by Klebsiella spp. (11.5% and 21.9%) in IAIs and UTIs, respectively. Figures for Pseudomonas aeruginosa were 9.0% and 6.1%, being more frequently recovered from patients with nosocomial infections. Overall, 9.9% (IAI) and 14.0% (UTI) of E. coli, Klebsiella spp. and Proteus mirabilis isolates were ESBL-producers, being Klebsiella pneumoniae (34.5%) from UTI of nosocomial origin the most frequent. ESBL-producers were higher in patients >60 years in both IAIs and UTIs. As in previous years, amikacin (96.3%-100% susceptibility), ertapenem (84.2%-100%) and imipenem (70.3%- 100%) were the most active antimicrobials tested among Enterobacterales species. The activity of amoxicillin-clavulanic, piperacillin-tazobactam, and ciprofloxacin susceptibility was lower, particularly among ESBL-producers. Ertapenem susceptibility (88.9%-100%) was retained in ESBL-E. coli isolates that were resistant to these antimicrobials but decreased (28.6%-100%) in similar isolates of K. pneumoniae. CONCLUSIONS: Continuous antimicrobial resistance surveillance from the SMART study reveals overall maintenance of ESBL-producers in Spain, although with higher presence in isolates from UTIs than from IAIs. Moreover, ertapenem activity was high in E. coli irrespective of ESBL production but decreased in K. pneumoniae, particularly among ESBL-producers.

Antimicrobial susceptibility trends and evolution of isolates with extended spectrum ß-lactamases among Gram-negative organisms recovered during the SMART study in Spain (2011-2015).

OBJECTIVE: The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study monitors antimicrobial susceptibility and extended spectrum ß-lactamases (ESBLs) in Gram-negative bacilli recovered from intra-abdominal infections (IAI). METHODS: Antimicrobial susceptibility of 5,343 isolates from IAI recovered in 11 centres during the 2011-2015 SMART-Spain program was analysed by standard microdilution (EUCAST criteria) and compared with that from 2002-2010. ESBLs were phenotypically detected. RESULTS: Escherichia coli, the most common isolate, significantly decreased in community acquired IAI (60.9% 2002-2010 vs. 56.1% 2011-2015, P=0.0003). It was followed in prevalence by Klebsiella pneumoniae that increased both in the community (8.9% vs. 10.8%, P=0.016) and nosocomial (9.2% vs. 10.8%, P=0.029) IAI and P. aeruginosa, which significantly increased in community acquired IAI (5.6% vs. 8.0%, P=0.0003). ESBLs were more prevalent in K. pneumoniae (16.3%) than in E. coli (9.5%) of nosocomial origin and were more frequently isolated from elderly patients (>60 years). Considering all Enterobacteriaceae, ertapenem (92.3-100%) and amikacin (95.5%-100%) were the most active antimicrobials. Ertapenem activity, unlike amoxicillin-clavulanate or piperacillin-tazobactam, remained virtually unchanged in ESBL (100%) and non-ESBL (98.8%) E. coli producers. Its activity decreased in ESBL-K. pneumoniae (74.7%) but was higher than that of amoxicillin-clavulanate (14.0%) and piperacillin-tazobactam (24.0%). Interestingly, ertapenem susceptibility was maintained in >60% of ESBL isolates that were resistant to amoxicillin-clavulanate, piperacillin-tazobactam or fluoroquinolones. CONCLUSIONS: SMART-Spain results support current guidelines which include ertapenem as empiric treatment in mild-moderate community-acquired IAI, particularly with ESBL producers. These recommendations will need to be updated with the recently introduction of new antimicrobials.

Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples.

Immunochromatographic (IC) tests may play an important role in the future diagnosis of parasitic diseases because of their speed and simplicity of use. A recently developed test to detect Cryptosporidium spp, Giardia duodenalis and Entamoeba histolytica was evaluated. Microscopy and PCR were the "gold standard" reference techniques and the results of this IC test were compared with those obtained with ELISA and IC single test for the three parasites. One hundred sixty stool samples were assayed. Using microscopy, 22 samples were diagnosed as positive for Cryptosporidium spp., 31 for Giardia duodenalis, 41 for Entamoeba histolytica/dispar, and 68 had a negative diagnosis for the three parasites. Results of IC tests show sensitivities of 70-72% for Cryptosporidium, 90-97% for Giardia and 62.5% for Entamoeba histolytica. Specificities were of 93.6-94.9%, >99% and 96.1%, respectively. In all diagnoses, agreement with microscopy and PCR was over 90%, except in the triple test and microscopy in E. histolytica detection that was 76.3%, due to the inability of microscopy to differentiate E. histolytica from nonpathogenic species such as E. dispar or E. moshkovskii. The triple stool immunoassays provide adequate sensitivities and specificities for use in outbreak situations, for screening proposals and for massive assays in endemic areas where a large number of samples must be analysed or as complementary test for individual diagnosis.

Evaluation of four phenotypic methods to detect plasmid-mediated AmpC ß-lactamases in clinical isolates.

Four phenotypic methods (three dimensional test, AmpC test, cloxacillin synergy test and cefotetan/cefotetan-cloxacillin E-test) to detect plasmid-mediated AmpC ß-lactamases (pAmpC) were compared in 125 clinical Enterobacteriaceae isolates with AmpC profile: 74 E. coli (bla (CMY-2): 70; bla (DHA-1): 4), five K. pneumoniae (bla (CMY-2): 2; bla (DHA-1): 3), six P. mirabilis (bla (CMY-2): 6) and 40 negative isolates for pAmpC ß-lactamases. All evaluated methods showed a good sensitivity (>95%) but low values of specificity (<60%) in E. coli, explained by an increase of AmpC expression caused by chromosomal ampC promoter/attenuator mutations (-42, -18, -1, +58, predominantly). The cefotetan/cefotetan-cloxacillin or cloxacillin synergy test may be advocated as phenotypic screening test, and the AmpC test as confirmatory test for detection of pAmpC in isolates that lack or minimally express chromosomally encoded AmpC ß-lactamases. In the case of E. coli, the phenotypic evaluated tests were not able to differentiate between chromosomal ampC overexpression or acquisition of plasmid-encoded ampC genes.

Detection and characterization of a ST6 clone of vanB2-Enterococcus faecalis from three different hospitals in Spain.

Thirteen vancomycin-resistant and teicoplanin-susceptible Enterococcus faecalis isolates were recovered from unrelated patients in three Spanish hospitals from November 2009 to December 2010. All isolates carried the vanB2 gene, showed indistinguishable or closely-related PFGE patterns and were ascribed to the sequence type ST6 (included into the high-risk clonal-complex CC2). They showed a multiresistance phenotype (erythromycin, tetracycline, ciprofloxacin and high-level-resistance to streptomycin, gentamicin and kanamycin) and harboured the aac(6')-aph(2"), ant(6)-Ia, and tet(M)+/-tet(L) genes. All isolates produced gelatinase and harboured the gelE gene, but not the esp or hyl genes. The inclusion of the vanB2 gene into the Tn5382 transposon was demonstrated in one isolate. Clonal dissemination of vanB2-containing the E. faecalis strain is demonstrated.

Plasmid-mediated QnrS2 determinant in an Aeromonas caviae isolate recovered from a patient with diarrhoea.

A qnrS2 gene was identified in an Aeromonas caviae isolate (MICs of ciprofloxacin, norfloxacin and ofloxacin >32 mg/L) from a stool sample collected from a patient with gastroenteritis. The analysis of the gyrA and parC genes revealed amino acid substitutions Ser83-Ile and Ser80-Thr, respectively. In addition, five out of 41 nalidixic acid-resistant Aeromonas isolates studied (26 identified as Aeromonas veronii bv sobria and 15 identified as A. caviae) showed ciprofloxacin resistance. The identification of plasmid-mediated qnr genes outside of the Enterobacteriaceae underlines a possible diffusion of these resistance determinants among Gram-negative rods. This emphasizes the importance of monitoring the emergence of these determinants as well as their dissemination among the Aeromonadaceae.

Is Rotavirus contributing to an increase of diarrhoea in a region of Spain?

Diarrhoeal illnesses are the most frequent of notifiable diseases in Aragon. Physicians notify diarrhoea cases with presumed infectious origin on a weekly basis. Following an increase in 2005-2006, we aimed to identify the responsible organism(s) in order to inform control measures. We described seasonality of diarrhoea notifications for 1998-2004 and 2005-2006. We calculated correlations between diarrhoea notifications and enteric pathogens diagnosed in two Aragonese laboratories, and applied linear regression using coefficients of determination (r2). In 2005-2006 the winter peak of diarrhoea notifications increased from 2494 to 3357 weekly cases (34.6%) and the peak in Rotavirus diagnoses from 15 to 39 weekly cases. The correlation of diarrhoea notifications with Rotavirus was 0.05 in 1998-2004 and 0.42 in 2005-2006. The model for 1998-2004 included Salmonella enterica, Giardia lamblia and Clostridium difficile (r2=0.08) and for 2005-2006 Rotavirus and Astrovirus (r2=0.24). Our results suggest that Rotavirus contributed to the increase of diarrhoea notifications. We recommend determining the disease burden of Rotavirus in order to guide vaccination policies.

Correlation between the presence of symptoms and the Giardia duodenalis genotype.

Clinical manifestations of Giardia duodenalis infection vary from asymptomatic infection to chronic diarrhoea. We study the correlation between the presence of symptoms and the G. duodenalis genotype in 108 patients with giardiasis. Patient age ranged from 2 to 72 years old. We found a correlation between assemblage AII and symptomatic infections, and between assemblage B and asymptomatic infections in the overall patient group and in patients less than five years of age. Nevertheless, if only patients of more than five years of age were considered, no statistically significant relationship between assemblage and symptomatic or asymptomatic Giardia infections was found. In these patients, host factors may affect the presence of clinical manifestations more than Giardia assemblage.

Salmonella enteríca portadora de betalactamasas de espectro extendido (blee) aisladas en Zaragoza durante los años 2001-2005 / No disponible

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Caracterización de enterobacterias portadoras de Blee aisladas de muestras invasivas / No disponible

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Mecanismos de resistencia a quinolonas / No disponible

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[Surveillance of commensal flora evolution and infections in neutropenic cancer patients submitted to chemoprophylaxis]. / Vigilancia de la evolución de la flora comensal y de las infecciones en pacientes oncológicos con neutropenia sometidos a quimioprofilaxis.

The evolution of the flora and its resistance to different antimicrobials in neutropenic patients submitted to high-dose chemotherapy with autologous blood stem-cell transplantation, and the relation of these findings to the etiology of the infections the patients developed was studied in order to evaluate the suitability of the chemoprophylaxis and the empirical antibiotic therapy used. Forty-one patients were analyzed in a period of 28 months. The chemoprophylaxis used was levofloxacin, fluconazole and acyclovir. The empirical sequential treatment was an initial administration of cefepime, followed by teicoplanin and amikacin. Cultures were done of nasal and pharyngeal smears, Hickman catheter and stools, 1 day before chemoprophylaxis started and then on days 5 and 9. In the case of fever, three sets of blood cultures and urine cultures were done and samples from areas related to the clinical condition were analyzed. Levofloxacin induced the selection of resistant strains or species in the flora and in the infectious agents. Fluconazole also selected resistant species in the flora. Seventeen infections were documented in eleven patients, produced by Gram-positive bacteria in thirteen cases (81.25%) and by Gram-negative bacteria in three (18.75%). The coagulase negative staphylococci and Enterococcus faecalis were the most frequent agents of infection. We identified on nine occasions the same microorganism in the flora and in the pathological product; this suggests its endogenous origin and supports the use of prospective cultures of the flora, monitoring the sensibility of the microorganisms isolated to the antimicrobials used in chemoprophylaxis and empirical treatment.

Vigilancia de la evolución de la flora comensal y de las infecciones en pacientes oncológicos con neutropenia sometidos a quimioprofilaxis / Surveillance of comensal flora evolution and infections in neutropenic cancer patients submitted to chemoprophylaxis

Estudiamos cómo evoluciona el tipo de flora comensal y su resistencia a distintos antimicrobianos en pacientes neurotropénicos sometidos a quimioterapia de altas dosis con autotrasplante de células madre autólogas hematopoyéticas periféricas, relacionando los hallazgos con la etiología de las infecciones que desarrollaron los pacientes, a fin de evaluar la idoneidad de la quimioprofilaxis y del tratamiento empírico utilizados. Se analizaron 41 pacientes en un periodo de 28 meses. La quimioprofilaxis se realizó con levofloxacino, fluconazol y aciclovir. El tratamiento empírico secuencial preveía la administación inicial de cefepima, seguida de teicoplanina y amikacina. Se realizaron cultivos de frotis nasales y faríngeo, catéter de Hickman y heces, un día antes de comenzar la quimioprofilaxis, a los cinco y nueve días. En caso de fiebre se realizaron tres hemocultivos y cultivo de orina y de muestras procedentes de focos relacionados con la clínica. El levofloxacino indujo la selección de cepas o especies resistentes, tanto en la flora comensal como en los agentes patógenos. El fluconazol seleccionó especies resistentes en la flora comensal. Se documentaron microbiológicamente 17 infecciones en 11 pacientes, producidas por gram positivos en 13 casos (81.25%) y por gramnegativos en 3 (18,75%). Los estafilococos coagulasa negativos y Enterococcus faecalis fueron los microorganismos más frecuentes. En nueve ocasiones recuperamos el mismo microorganismo en flora comensal y producto patológico, lo que sugiere su origen endógeno y apoya la realización prospectiva de cultivos de flora comensal, vigilando la sensibilidad de los microorganismos aislados a los antimicrobianos usados en quimioprofilaxis y tratamiento empírico

The evolution of the flora and its resistance to different antimicrobials in neutropenic patients submitted to high-dose chemotherapy with autologous blood stem-cell transplantation, and the relation of these findings to the etiology of the infections the patients developed was studied in order to evaluate the suitability of the chemoprophylaxis and the empirical antibiotic therapy used. Forty-one patients were analysed in a period of 28 months. The chemoprophylaxis used was levofloxacin, fluconazole and acyclovir. The empirical sequential treatment was an initial administration of cefepime, followed by teicoplanin and amikacin. Cultures were done of nasal and pharyngeal smears, Hickman catheter and stools, 1 day before chemoprophylaxis started and then on days 5 and 9. In the case of fever, three sets of blood cultures and urine cultures were done and samples from areas related to the clinical condition were analysed. Levoflaxacin induced the selection of resistant strains or species in the flora and in the infectious agents. Fluconazole also selected resistant species in the flora. Seventeen infections were documented in eleven patients, produced by Gram-positive bacteria in thirteen cases (81.25%) and by Gram-negative bacteria in three (18.75%). The coagulase negative staphylococci and Enterococcus faecalis were the most frequent agents of infection. We identified on nine occasions the same microorganism in the flora and in the pathological product; this suggest its endogenous origin and supports the use of prospective cultures of the flora, monitoring the sensibility of the microorganisms isolated to the antimicrobials used in chemoprophylasis and empirical treatment