A prime-boost vaccination of mice with attenuated Salmonella expressing a 30-mer peptide from the Trichinella spiralis gp43 antigen.

Protection against Trichinella infections has been achieved using various parasite antigens and adjuvants. Recently, we reported that immunization of mice with an attenuated Salmonella strain displaying a 30-mer peptide (residues 210-239) from the Trichinella spiralis gp43 antigen using the ShdA autotransporter induced partial protection against T. spiralis infection. To improve the efficacy of vaccination, we used the MisL autotransporter system to display the Ts30mer peptide on the surface of Salmonella enterica ser. Typhimurium in combination with a prime-boost vaccination strategy. This vector and immunization regimen induced superior protection against T. spiralis when compared to our previously reported approach. Data presented herein showed a significant reduction in adult worm and muscle larvae burdens, high IgG titers, and increased production of intestinal mucus with entrapped adult worms. This prime-boost vaccination scheme is a suitable strategy to elicit enhanced protective immunity against T. spiralis.

[Phenotype markers in strains of Aeromonas isolated in Cuba from children with an acute diarrheic disease]. / Marcadores fenotípicos en cepas de Aeromonas aisladas en Cuba de niños con enfermedad diarreica aguda.

Twenty-seven cases of Aeromonas isolated from 300 five-years children with acute diarrheal disease (EDA) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. The percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher than than 1:16. The presence of markers in Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae was demonstrated.

[Inhibition of the multiplication of a Coxsackie A9 strain isolated from a patient with epidemic neuropathy by the interferons alfa and gamma]. / Inhibición de la multiplicación de la cepa de Coxsackie A9 aislada de un paciente con neuropatía epidémica frente a los interferones alfa y gamma.

We present the results of the in vitro action of alpha and gamma interferons and of Intacglobin and Igegam against the 47/93/IPK (Coxsackie A9) strain isolated from the cerebrospinal fluid of a patient with epidemic neuropathy. The in vitro studies showed that the two interferons inhibited the replication of this agent; they also showed the presence of antibodies to it in the Intacglobin and Igegam. The results attained demonstrated that the use of these compounds could be effective for the treatment of this entity.

[Establishment of a subline of C6/36 adapted to growing in a serum-free medium and its usefulness for studies on the dengue virus]. / Obtención de una sublínea de C6/36 adaptada a crecer en medío libre de suero y su utilización para estudios sobre virus del dengue.

Using the C6/36 clone of the Aedes albopictus cell line, a derived cell line was obtained capable of growing in a commercial serum-free medium facilitating this line to keep its morphologic characteristics and adhesion capacity. The growth speed rate reduction of the adapted cell line was compensated by its low maintenance cost. Its usefulness in the multiplication of dengue viruses 1 and 2 was tested; the reported line showed the same susceptibility for the 2 agents than the original cell line when the indirect fluorescence technique was applied.

[Normalization of an ultramicroELISA for the detection of antibodies against human Parvovirus B19]. / Normalización de un ultramicroELISA para la detección de anticuerpos a Parvovirus humano B19.

An ultramicroELISA (UME) method was normalized for the detection of antibodies to serum human parvovirus B19. The optimum antigen concentration determined was 400 ng/ml, serum dilution was of 1:100; and the conjugate work dilution was of 1:2,000. 11 paired serum samples were also evaluated and antibodies were detected. The usefulness of the analyzed system is discussed.

[Obtaining a clone of the AP-61 cell line. Its usefulness in the multiplication of dengue viruses 1 and 2]. / Obtención de un clono de la línea celular AP-61. Su utilidad para la multiplicación de los virus dengue 1 y 2.

The obtention of a clone of the cell line AP-61 (Aedes pseudoscutellaris) is reported. Details of the cloning and culture media employed are discussed. Usefulness of the clone for the multiplication of dengue 1 and 2 viruses is proved in comparison with the original line using the indirect immunofluorescence technique.

[Establishment of a diploid cell line of human lung (PHuE-1)]. / Obtención de una línea celular diploide de pulmón humano (PHuE-1).

The obtention of a diploid cell line from human lung of great concern for the virological diagnosis and research is reported. Certain aspects about its application and characterization are discussed.

[Use of goat serum as a substitute for calf serum for growing various primary cultures from vertebrates]. / Utilización del suero caprino como sustituto del suero de ternera donante en el crecimiento de algunos cultivos primarios de vertebrados.

Goat's serum was used to prepare primary cultures of fibroblast from chicken embryo and hamster kidney. Later they were inoculated the East equine encephalomyelitis virus, being compared with culture from calf's serum. The choice of goat's serum is of great interest for the development of scientific research and national economy.

[Preliminary study of AP-64 cell line (Aedes pseudoscutellaris) for dengue-1 and -2 virus multiplication]. / Estudio preliminar de la línea celular AP-64 (Aedes pseudoscutellaris) para la multiplicación de los virus dengue-1 y 2.

We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.

[Effect of various factors during cryopreservation on the morphology and viability of several poikilothermic cell lines]. / Efecto de varios factores durante la criopreservación sobre la morfología y la viabilidad de algunas líneas celulares poikilotérmicas.

This paper deals with the effect of several factors, during cryopreservation, on three poikilothermic cell lines using morphology and viability as indicators. It was proved that to avoid toxicity in the suspension medium is of vital importance for the outcome of freezing. In addition, one of the systems studied and of great application in Virology does not bear long hibernations. Finally, the most adequate freezing method for this type of cells is discussed.