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Reprod Domest Anim ; 54 Suppl 4: 54-58, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31625233

RESUMO

The aim of this study was to develop a non-invasive procedure to stimulate ejaculation in median and small lizards for semen collection. After semen collection, we applied a battery of tests to assess the motility (wave and progressive), viability (eosin-nigrosin stain), morphology (normal/abnormal), sperm concentration and ejaculate number, seminal volume and colour. We obtained this fluid from all males of the four species of sceloporine lizards (n = 30) and one species of Mexican horned lizards (n = 7). We found that semen from all males had a liquid-like consistency and a milky-white appearance. Ejaculate volume and sperm concentration varied among the males and the species studied. We also observed that although the mobility, viability and normal sperm morphology showed a wide variability, their average value in each species was high. We conclude that "genital papilla pressure" was an efficient and non-invasive semen obtaining method for small- and medium-sized lizards that does not imply damage to donors and could be used in other lizard species.


Assuntos
Lagartos/fisiologia , Sêmen , Testículo/fisiologia , Coleta de Tecidos e Órgãos/veterinária , Animais , Ejaculação/fisiologia , Masculino , Motilidade dos Espermatozoides , Espermatozoides/citologia , Coleta de Tecidos e Órgãos/métodos
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