A transimpedance preamplifier using a feedforward approach for robust rejection of DC photogenerated currents.

The preamplifier proposed in this paper is designed to extract weak variable photogenerated signals from a high-level continuous background ensuring low noise and high transimpedance gain. An efficient cancellation of the DC component directly at the photodetector output, exploiting a feedforward approach, allows us to properly amplify the variable signal components of interest avoiding saturation of the preamplifier. Furthermore, the large transimpedance gain allows for minimizing the effects of the noise introduced by the following stages on the signal processing chain. In the paper, we present the proposed approach and a possible circuit realization with a signal AC/DC ratio as small as 1/1000 ensuring low noise, high gain, and a considerable bandwidth. The realized preamplifier offers a Noise Equivalent Power NEP ≃ 1.12 nW, an in-band transimpedance gain of 4.4 MΩ, and a wide bandwidth from about 1 Hz up to 100 kHz, making it suitable for use in several applications both in biomedical and industrial fields.

Light-storing photocatalyst: the possibility of activating titanium dioxide by photoluminescence.

INTRODUCTION: The aim of the present work was to analyze the possibility of creating a light-storing photocatalyst material, a system that couples a phosphorescent substrate with a titania coating. The substrate could activate the titanium dioxide coating even in condition of darkness thanks to its light emission. This improvement of absorption can be achieved because the titania semiconductor is irradiated both by the direct light and by the luminescent substrate which is activated at the same time. METHODS: TiO2-based sol-gel solutions were deposited on commercial borosilicate glasses containing 2 different phosphorescent pigments (emission peaks at 470 and 520 nm, respectively). Particular attention was paid to optimize the film-substrate adhesion by using a scratch test to evaluate the optimal temperature of the post- treatment step. RESULTS AND DISCUSSION: The main result is that when irradiated the photoluminescent pigments allow an increase in the photocatalytic acitivity of the coatings. The pigments are able to convert the ultraviolet component of light in order to increase the amount of energy that can be absorbed by titania. Moreover, the hydrophilicity test showed that in the absence of irradiation the phosphorescent samples are characterized by a lower loss of superhydrophilicity.

In vivo real time non invasive monitoring of brain penetration of chemicals with near-infrared spectroscopy: Concomitant PK/PD analysis.

BACKGROUND: Near-infrared spectroscopy (NIRS) is a non-invasive technique that monitors changes in oxygenation of haemoglobin. The absorption spectra of near-infrared light differ for the oxygenation-deoxygenation states of haemoglobin (oxygenate (HbO2) and deoxygenate (Hb), respectively) so that these two states can be directly monitored. COMPARISON WITH EXISTING METHOD(S): Different methodologies report different basal values of HbO2 and Hb absolute concentrations in brain. Here, we attempt to calculate basal HbO2 levels in rat CNS via evaluation of the influence of exogenous oxygen or exogenous carbon dioxide on the NIRS parameters measured in vivo. NEW METHOD: Furthermore the possibility that changes of haemoglobin oxygenation in rat brain as measured by NIRS might be a useful index of brain penetration of chemical entities has been investigated. Different compounds from different chemical classes were selected on the basis of parallel ex vivo and in vivo pharmacokinetic (PK/PD) studies of brain penetration and overall pharmacokinetic profile. RESULTS: It appeared that NIRS might contribute to assess brain penetration of chemical entities, i.e. significant changes in NIRS signals could be related to brain exposure, conversely the lack of significant changes in relevant NIRS parameters could be indicative of low brain exposure. CONCLUSIONS: This work is proposing a further innovation on NIRS preclinical applications i.e. a "chemical" NIRS [chNIRS] approach for determining penetration of drugs in animal brain. Therefore, chNIRS could became a non invasive methodology for studies on neurobiological processes and psychiatric diseases in preclinical but also a translational strategy from preclinical to clinical investigations.

An in vitro model of a system of electrical potential compensation in extracorporeal circulation.

OBJECTIVES: Extracorporeal circulation (ECC) in patients undergoing cardiac surgery induces systemic immune-inflammatory reaction that results in increased postoperative morbidity. Many factors are responsible for the adverse response after ECC. The present in vitro study aimed to investigate electric charges (ECs) generated during ECC, to set a device compensating the ECs, and checking its effect on red blood cells (RBC). MATERIALS AND METHODS: The electrical signals of blood in ECC were collected by a custom developed low-noise electronic circuit, processed by a digital oscilloscope (DSO) and a dynamic signal analyzer (DSA). The compensation of ECs was performed using a compensation device, injecting a nulling charge into the blood circuit. The compensation effect of the ECs on RBCs was evaluated by scanning electron microscope (SEM). RESULTS: The electrical analysis performed using both the DSO and the DSA confirmed the EC formation during ECC. The notable electric signals recorded in standard ECC circuits substantially nulled once the compensation device was used, thus confirming efficient EC compensation. After two hours of ECC, the SEM non-blended test on human RBC samples highlighted morphological changes in acanthocytes of the normal biconcave-shaped RBC. CONCLUSIONS: The outcomes confirm the development of parasitic ECs during ECC and that a suppressor system may decrease the potential damage of ECs. Nevertheless, further studies are ongoing in order to investigate the complex mechanisms related to lymphocytes and platelet morphological and physiological chances during triboelectric charges in ECC.

In vivo diffuse correlation spectroscopy investigation of the ocular fundus.

Diffuse correlation spectroscopy (DCS) measurements in vivo recorded from rabbits' ocular fundus are presented. Despite the complexity of these ocular tissues, we provide a clear and simple demonstration of the DCS abilities to analyze variations in physiological quantities of clinical interest. Indeed, the reported experimental activities demonstrate that DCS can reveal both choroidal-flow and temperature variations and detect nano- and micro-aggregates in ocular fundus circulation. Such abilities can be of great interest both in fundamental research and practical clinical applications. The proposed measuring system can be useful in: (a) monitoring choroidal blood flow variations, (b) determining the end-point for photo-dynamic therapy and transpupillary thermo therapy and, (c) managing the dye injection and determining an end-point for dye-enhanced photothrombosis. Moreover, it could allow both diagnoses when the presence of nano- and micro-aggregates is related to specific diseases and verifying the effects of nanoparticle injection in nanomedicine. Even though the reported results demonstrate the applicability of DCS to investigate ocular fundus, a detailed and accurate investigation of the limits of detection is beyond the scope of this article.

Development of a sensor system for the determination of sanitary quality of grapes.

n instrument for the automatic quantification of glycerol in grapes has been developed. We verify here that this analyte can be used as a benchmark of a serious disease affecting the grapevines, namely Botrytis cinerea. The core of the instrument is an amperometric biosensor consisting of a disposable screen printed electrode, generating the analytical signal thanks to a bi-enzymatic process involving glycerol dehydrogenase and diaphorase. The full automation of the analysis is realised by three micropumps and a microprocessor under control of a personal computer. The pumps allow the correct and constant dilution of the grape juice with a buffer solution also containing [Fe(CN)6]3- redox mediator and the injection of NAD+ cofactor when the baseline signal reaches a steady state; the instrument leads to automated reading of the analytical signal and the consequent data treatment. Although the analytical method is based on an amperometric technique that, owing to heavy matrix effects, usually requires an internal calibration, the analyses indicate that a unique external calibration is suitable for giving accurate responses for any grapes, both white and black ones.

A simple readout electronics for automatic power controlled self-mixing laser diode systems.

The paper describes a simple electronic circuit to drive a laser diode for self-mixing interferometry. The network integrates a stable commercial automatic power controller and a current mirror based readout of the interferometric signal. The first prototype version of the circuit has been realized and characterized. The system allows easily performing precise interferometric measurements with no thermostatic circuitry to stabilize the laser diode temperature and an automatic control gain network to compensate emitted optical power fluctuations. To achieve this result, in the paper a specific calibration procedure to be performed is described.