RESUMO
BACKGROUND: Androgen receptor [CAG](n) microsatellite has been linked to human diseases. METHODS: Six non-human primates were genotyped for the [CAG](n) microsatellite. RESULTS: Marmosets and macaques are monomorphic, while mangabeys, baboons, and chimpanzees are polymorphic. CONCLUSIONS: Non-human primates that are polymorphic for the microsatellite are candidate animal models for CAG-related diseases.
Assuntos
Primatas/genética , Receptores Androgênicos/genética , Repetições de Trinucleotídeos , Animais , DNA/química , DNA/genética , Masculino , Pan troglodytes , Reação em Cadeia da Polimerase/veterinária , Polimorfismo GenéticoRESUMO
BACKGROUND: An inverse relationship between serum prostate specific antigen (PSA) levels and body mass index (BMI) has been reported in men but not in any animal model. METHODS: Serum PSA in a colony of cynomolgus monkeys was assayed and correlated to body weight, prostate weight, and age. In addition, 15 animals were selected and fed a high sugar high fat (HSHF) diet for 49 weeks to increase their BMI and correlate it to PSA RESULTS: Serum PSA levels were positively correlated to prostate weight (r = 0.515, P = 0.025) and age (r = 0.548, P = 0.00072) but was not significantly correlated to body weight (r = -0.032, P = 0.419). For the animals on the HSHF diet, body weight, lean mass, fat mass, and BMI were significantly higher at 49 weeks than at baseline (P < 0.01). PSA was not significantly correlated to body weight and insulin at both baseline and 49 weeks. PSA was negatively correlated to BMI and insulin resistance (HOMA-IR) at 49 weeks but not at baseline. In addition, we observed hepatic steatosis and increases in serum liver enzymes. CONCLUSIONS: Increases in BMI in cynomolgus monkeys as a result of consuming a HSHF diet resulted in PSA changes similar to those in humans with increased BMI. Cynomolgus monkeys are a useful model for investigating the relationship between obesity, diabetes, and PSA changes resulting from prostate gland pathology.
Assuntos
Carboidratos da Dieta/efeitos adversos , Gorduras na Dieta/efeitos adversos , Macaca fascicularis/metabolismo , Obesidade/sangue , Antígeno Prostático Específico/sangue , Absorciometria de Fóton , Animais , Glicemia/análise , Índice de Massa Corporal , Peso Corporal/efeitos dos fármacos , Carboidratos da Dieta/administração & dosagem , Gorduras na Dieta/administração & dosagem , Modelos Animais de Doenças , Insulina/sangue , Resistência à Insulina , Masculino , Obesidade/etiologia , Obesidade/patologia , Tamanho do Órgão/efeitos dos fármacos , Próstata/efeitos dos fármacos , Próstata/metabolismo , Próstata/patologiaRESUMO
BACKGROUND: The metabolic syndrome is common in populations exposed to a typical Western diet. There is a lack of an animal model that mimics this condition. METHODS: We fed 15 cynomolgus monkeys ad libitum a high-sugar high-fat (HSHF) diet for 33 weeks. Body weight, body composition, serum lipids, and insulin were measured at baseline and at 33 weeks. RESULTS: The animals tolerated the HSHF diet very well. In the intervention group, total serum cholesterol and low-density lipoprotein cholesterol were 3- and 5-fold higher, respectively, at 33 weeks as compared with their baseline levels. Serum high-density lipoprotein cholesterol and triglycerides were not significantly affected. Dual-energy X-ray absorptiometry (DXA) analysis of the intervention group indicated that the trunk fat mass increased by 187% during this period. CONCLUSIONS: Cynomolgus monkeys should be a useful model for investigating the interactions of diet and other factors such as genetics in the development of the metabolic syndrome.
