1.
Methods Mol Biol
; 1843: 189-201, 2018.
Artigo
em Inglês
| MEDLINE
| ID: mdl-30203288
RESUMO
The combination of single-molecule fluorescence imaging and electrophysiology provides a powerful tool to explore the stoichiometry and functional properties of ionic channels, simultaneously. Here, we describe a typical SC-SMD experiment from the preparation of plasmids containing the genes encoding for the channels of interest fused to fluorescent proteins to the use of the SC-SMD system for simultaneous patch clamping and single-molecule determinations.