RESUMO
The objective of this study was to obtain information about the role of trace element imbalance in the pathogenesis of certain diseases in dogs and to evaluate the suitability of trace element profiling as an additional tool in the diagnosis. Serum trace element concentrations (copper, molybdenum, selenium and zinc) were measured in a cohort of healthy (control) dogs (n = 42) and dogs affected by hepatic (n = 25), gastrointestinal (n = 24), inflammatory/infection (n = 24), and renal (n = 22) diseases. These data were analyzed together with data on basic biochemical parameters (alanine aminotransferase, alkaline phosphatase, blood urea nitrogen, creatinine, albumin, globulin, and glucose) by using chemometric techniques. The chemometric analysis revealed distinctive association patterns between trace elements and biochemical parameters for each clinical disorders. The findings provide clear evidence for the important role of trace elements in disease, particularly in relation to acute phase reactions, with serum copper providing an indirect measurement of ceruloplasmin (positive acute-phase protein) and serum selenium and zinc acting as negative acute phase reactants. Molybdenum may also be a suitable marker of incipient renal disease. Thus, the analysis of trace element profiles, by multielement techniques, in a single serum sample would be a valuable additional tool for the diagnosis of certain diseases.
RESUMO
This study was designed (i) to establish reference ranges for the essential trace element and background levels of toxic element exposure in the healthy/normal dog population, and (ii) to evaluate whether trace element concentrations vary in dogs suffering from different pathologies. Blood serum samples were collected from 187 healthy and diseased dogs at the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Santiago de Compostela (northwest Spain). The samples were acid digested, and the concentrations of trace elements (Co, Cr, Cu, Fe, Mn, Mo, Ni, Se and Zn) and toxic elements (As, Cd, Hg and Pb) were determined by inductively coupled plasma-mass spectrometry (ICP-MS). This enabled us to establish reference ranges for the essential trace elements and the level of toxic element exposure in dogs, and to identify several clinical situations associated with variations in trace elements in serum. Relative to concentrations in healthy control dogs, statistically significant differences were observed in the concentrations of Cu (significantly higher in hepatic, inflammatory/infectious and oncological categories), Mo (significantly higher in renal category), Se (significantly lower in gastrointestinal category) and Zn (significantly lower in gastrointestinal, inflammatory/infectious and renal categories). Trace element concentrations can be a cause or consequence of disease, and the study findings indicate that trace element determination in serum provides useful information on the pathogenesis of certain diseases. Further research on the serum concentrations of trace elements, particularly in relation to other biochemical parameters and diagnostic tools, may provide valuable information for the diagnosis of diseases in dogs and the disease prognosis.
RESUMO
This study was designed to evaluate the influence of type of blood sample (serum or plasma) on essential and toxic element analysis in cattle. Paired plasma and serum samples (n = 20) were acid digested, and the concentrations of As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn. Mo, Ni, P, Pb, Sb, Se, Sr and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). The study findings indicate that plasma and serum samples appear suitable and interchangeable for the determination of most of the essential and toxic elements in blood in cattle. The only exceptions are Cu and Se, the concentrations of which were significantly lower (40.9 and 29.9% respectively) in serum than in plasma. Some of the Cu in blood samples from bovine ruminants is known to be sequestered during clotting. However, further research on Se in ruminants and other animal species is warranted. Finally, the significantly higher Mn (9.9%) concentrations in serum than in plasma may have been caused by haemolysis of some samples. Special attention should be paid to preventing haemolysis of samples during collection and processing, in order to prevent overestimation of elements present at high concentrations inside erythrocytes (i.e., Fe, Mn and Zn).
RESUMO
Se estudiaron 16 pacientes, a cada uno se le administraron 200 mg de TRH y se le determinó hGH a los -15, 0, 15, 30, 60, 90, 120 minutos, además, una prueba de sobrecarga a la glucosa para determinar glicemia y hGH a los 0, 30, 60, 120 minutos. Se encontró que siete pacientes no liberaron hGH pos-TGH (todos presentaron respuesta normal a la sobrecarga de glucosa). En nueve pacientes hubo respuesta de hGH a la TRH, de ellos 3 tenían respuesta normal possobrecarga de glucosa. Se considera que esta prueba podría: 1, servir como criterio de curación; 2, tener valor pronóstico y 3, permitir profundizar en la patogenia de la enfermedad
Assuntos
Humanos , Acromegalia , Hormônio do Crescimento , Hormônio Liberador de TireotropinaRESUMO
Se estudiaron 16 pacientes, a cada uno se le administraron 200 mg de TRH y se le determinó hGH a los -15, 0, 15, 30, 60, 90, 120 minutos, además, una prueba de sobrecarga a la glucosa para determinar glicemia y hGH a los 0, 30, 60, 120 minutos. Se encontró que siete pacientes no liberaron hGH pos-TGH (todos presentaron respuesta normal a la sobrecarga de glucosa). En nueve pacientes hubo respuesta de hGH a la TRH, de ellos 3 tenían respuesta normal possobrecarga de glucosa. Se considera que esta prueba podría: 1, servir como criterio de curación; 2, tener valor pronóstico y 3, permitir profundizar en la patogenia de la enfermedad
