Altered growth and metabolism of an estuarine shrimp (Palaemonetes pugio) during and after metamorphosis onto fenvalerate-laden sediment.

Dry weight (W), carbon (C), nitrogen (N), and energy (E) (calculated) accumulation were measured in the estuarine grass shrimp, Palaemonetes pugio, throughout larval development and during the first 2 weeks as postlarvae in seawater over sediment containing the pyrethroid insecticide fenvalerate (SCF; nominal concentrations of 1, 10, and 100 microgram fenvalerate kg-1 sediment). The influence of fenvalerate-laden sediment on shrimp growth and utilization patterns of C, N, and E was dependent on fenvalerate concentration, age of shrimp, and whether shrimp were premetamorphic or postmetamorphic in development. The fenvalerate concentration in the sediment, which ultimately inhibited larval metamorphosis (100 microgram fenvalerate kg-1 sediment), significantly reduced W accumulation in developing larvae and in postlarvae growing on the sediment for an equivalent time. Accumulation of C, N, and E varied not only with concentration of SCF, but differed between pelagic larvae developing in water above SCF and newly settled postlarvae growing in direct contact with SCF. Larvae developing above >/=10 microgram kg-1 SCF contained significantly less N, while postlarval shrimp settling onto >/=10 microgram kg-1 SCF accumulated significantly less C and E. Measurable variations in growth and energy reserves of toxicant-sensitive life stages in response to environmentally realistic insecticide exposures have a direct link to ecological consequences of toxic stress and may be useful as biomarkers to diagnose early damage in estuarine populations.

Use of artificial sediments in a comparative toxicity study with larvae and postlarvae of the grass shrimp, Palaemonetes pugio.

An artificial sediment was tested for use in evaluating the potential hazard of toxicants on benthic organisms. The seawater-sediment system was assessed by use of the pyrethroid insecticide, fenvalerate, as the model toxicant for testing with larvae of the grass shrimp, Palaemonetes pugio, an ecologically important estuarine species. The sediment was prepared from commercially available components, and mixed with the toxicant to provide concentrations of 1, 10 and 100 microg fenvalerate kg(-1) dry sediment in 20 ppt seawater. Sediment free of the insecticide served as the control. Throughout the study, fenvalerate was not detected in the water column, but was measured in sediment at the nominal concentration of 100 microg kg(-1). The P. pugio population was adversely affected by fenvalerate. The effect occurred at metamorphosis, when larvae changed from pelagic individuals to benthic organisms. At this period, larvae were in direct contact with sediment. A portion of the population was tolerant of the insecticide.

The influence of an insect growth regulator on the larval development of the mud crab Rhithropanopeus harrisii.

Effects of the juvenile hormone analogue (S)-methoprene on the larval development and survival of the estuarine mud crab Rhitrhopanopeus harrisii were examined in the laboratory. Crab leave continuously exposed to 1000 microg (S)-methoprene litre(-1) did not survive beyond zoeal stage I. With continuous exposure to 100 microg (S)-methoprene litre(-1), mortality significantly increased through all larval stages except for zoeal stage II, and developmental duration significantly increased through all zoeal stages but not the premetamorphic megalopa. No supernumerary larval stages occurred with exposure (S)-methoprene. Rhithropanopeus harrisii larvae appear to be more sensitive to the single isomer formulation, (S)-methophrene, than to the double isomer formulation, (S)-methoprene, used in previously documented studies.