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Sci Rep ; 7(1): 14284, 2017 10 27.
Artigo em Inglês | MEDLINE | ID: mdl-29079771

RESUMO

Ultra-sensitive electrodes for extracellular recordings were fabricated and electrically characterized. A signal detection limit defined by a noise level of 0.3-0.4 µV for a bandwidth of 12.5 Hz was achieved. To obtain this high sensitivity, large area (4 mm2) electrodes were used. The electrode surface is also micro-structured with an array of gold mushroom-like shapes to further enhance the active area. In comparison with a flat gold surface, the micro-structured surface increases the capacitance of the electrode/electrolyte interface by 54%. The electrode low impedance and low noise enable the detection of weak and low frequency quasi-periodic signals produced by astrocytes populations that thus far had remained inaccessible using conventional extracellular electrodes. Signals with 5 µV in amplitude and lasting for 5-10 s were measured, with a peak-to-peak signal-to-noise ratio of 16. The electrodes and the methodology developed here can be used as an ultrasensitive electrophysiological tool to reveal the synchronization dynamics of ultra-slow ionic signalling between non-electrogenic cells.


Assuntos
Astrócitos/fisiologia , Potenciais da Membrana , Microeletrodos , Animais , Células Cultivadas , Córtex Cerebral/fisiologia , Capacitância Elétrica , Impedância Elétrica , Desenho de Equipamento , Compostos de Ouro , Camundongos Endogâmicos C57BL , Neurofisiologia/instrumentação , Cultura Primária de Células
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