Effect of association of non-steroidal anti-inflammatory and antibiotic agents with calcium hydroxide pastes on their cytotoxicity and biocompatibility.

OBJECTIVES: Evaluate the cytotoxicity and biocompatibility of calcium hydroxide paste [Ca (OH)2] associated with 5% diclofenac sodium, ibuprofen, or amoxicillin. MATERIALS AND METHODS: Pre-osteoblast-like cells were cultivated and the MTT test was used to determine the cytotoxicity of the paste extracts after time intervals of 24, 48, 72 h, and 7 days. Tubes containing Ca (OH)2 pastes associated with the drugs and empty tubes were implanted in subcutaneous tissue of 30 rats. After 7 and 30 days, the specimens were removed and submitted to histological analysis. The data obtained were submitted to Kruskal-Wallis and Dunn tests (p ≤ 0.05). RESULTS: All the Ca (OH)2 pastes promoted cell viability after all periods. At 7 days, there was greater inflammatory tissue reaction adjacent to the implants. At 30 days, there was a significant reduction in the number of inflammatory cells, and increase in fibroblasts in all groups. In this period, a lower number of inflammatory cells and a higher number of fibroblasts were observed in the capsules adjacent to the association with diclofenac, when compared with the other mixtures (p ≤ 0.05); the capsule thickness was greater at 7 days than at 30 days. CONCLUSIONS: The Ca (OH)2 pastes associated with the drugs were not cytotoxic and presented biocompatibility after implantation in rat subcutaneous tissues. CLINICAL RELEVANCE: Ca (OH)2 pastes with anti-inflammatory or antibiotic may be clinical alternatives as intracanal medication to reduce resistant microorganisms in root canal system.

Tratamento cirúrgico de perfuração radicular com obturação simultânea do canal radicular ­ relato de caso / Surgical treatment of a root perforation with simultaneous filling of root canal ­ case report

O insucesso do tratamento endodôntico é descrito como a persistência de sinais clínicos e radiográficos e da sintomatologia dolorosa pós-tratamento. O tratamento (cirúrgico ou não cirúrgico) e o prognóstico dependem da causa e das condições presentes do dente. O objetivo deste estudo foi relatar um caso clínico de fracasso de um tratamento endodôntico associado a uma perfuração radicular, relatando sua etiologia, diagnóstico, tratamento e acompanhamento. Paciente de 32 anos, sexo masculino, compareceu à clínica de Endodontia da Faculdade de Odontologia de Bauru, com queixa de desconforto na região de incisivo lateral superior direito, para avaliação. O exame radiográfico revelou presença de lesão periapical e obturação insatisfatória, enquanto que o exame clínico demonstrou presença de bolsa periodontal, discreta mobilidade, sensibilidade à percussão vertical e à palpação apical. O exame tomográfico mostrou perfuração na região vestibular da raiz associada a um extravasamento de material obturador, sugerindo ser a causa do fracasso do tratamento endodôntico primário. A cirurgia foi indicada como intervenção. A perfuração foi cirurgicamente exposta, limpa e selada com SuperEBA, enquanto que o canal foi simultaneamente retratado e obturado. No final, um enxerto ósseo e uma membrana foram utilizados para estimular uma regeneração tecidual guiada da região. Em um acompanhamento clínico e radiográfico de 4 anos, demonstrou-se reparo satisfatório do tecido ósseo com ausência de sintomatologia dolorosa. O diagnóstico preciso e o tratamento cirúrgico com obturação simultânea do canal radicular foram determinantes para o reparo satisfatório da lesão periapical e remissão dos sinais e sintomas de inflamação (AU).

The insucess of the endodontic treatment is described as the persistence of clinical and radiographic signs and painful symptomatology post-treatment. Its therapy (non-surgical and surgical treatment) and prognosis depend on the cause and tooth conditions. The aim of this study was report a case of an endodontic treatment failure associated with a root perforation retreating its etiology, diagnosis, management, and overcome. A 32-years old male patient was reffered to clinic of endodontics in Bauru School of Dentistry with complaint of discomfort in the region of maxillary right lateral incisor for evaluation. The radiographic revealed the presence of periapical lesion and unsatisfactory filling, while the clinical examination revealed the presence of periodontal pocket, discrete mobility, sensitivity to vertical percussion and to apical palpation. The tomographic examination showed a perforation on vestibular region of the root associated with filling material extravasation suggesting it be the cause of the failure of the primary endodontic treatment. The surgery was indicated as intervention. The perforation was surgically exhibited, cleaned up, and sealed with SuperEBA, while the root canal was simultaneously retreated and filled. Finally, a bone grafting and a membrane insertion were performed to induce tissue regeneration of the region. Four years follow up x-ray and clinical exam showed satisfactory repair of the hard tissue with absence of painful symptomatology. The correctly diagnosis and the surgical treatment with simultaneous filling of root canal were determinant for satisfactory repair of periapical lesion and the resolution of inflammation signs and symptomatology (AU).

Comparisons by microcomputed tomography of the efficiency of different irrigation techniques for removing dentinal debris from artificial grooves.

AIM: The aim of this study was compare the capacity of different irrigation protocols for debris removal from artificial grooves and assess the effectiveness of Easy Clean used in different kinematics by means of micro-CT. METHODOLOGY: Fifty acrylic prototyped maxillary incisors were instrumented and included in a muffle. The specimens were longitudinally sectioned, and a longitudinal groove was made on the inner surface of the root canal in one of hemisections, and the dentin debris was inserted into the grooves. The specimens were divided into five groups (n = 10): G1: Conventional with open-ended needle; G2: Conventional with double side-vented needle; G3: Easy Clean in reciprocating movement; G4: Easy clean in continuous rotation (ECCR); and G5: Passive ultrasonic irrigation (PUI). All specimens were scanned using microcomputed tomography before and after the irrigation technique and calculated the volume (mm3) of dentin debris. The paired t-test and post hoc Tukey test were the statistical tests used, with significance set at 5%. RESULTS: There were no significant difference (P > 0.05) between PUI and ECCR. ECCR was significantly (P < 0.05) more effective than the groups of conventional irrigation techniques. CONCLUSIONS: PUI and ECCR favored the removal of a larger volume of dentin debris from the groove.

Avaliação do pH, liberação de íons cálcio e atividade antimicrobiana do cimento Portland associado a Bambusa textilis em diferentes proporções / Analysis of pH, calcium ion release and antibacterial activity of Portland cement associated to Bambusa textilis in different proportion

Introdução: há uma busca incessante por agentes fitoterápicos na medicina e odontologia. Dentro da endodontia, busca-se uma substância que potencialize os efeitos biológicos e antimicrobianos do hidróxido de cálcio como curativo de demora. Não há na literatura relatos sobre a associação do cimento Portland com a Bambusa textilis, uma planta nativa da China, onde é usada na elaboração de medicamentos, pois se trata de uma fonte importante de resinas, sendo, portanto utilizadas por seu poder anti-inflamatório, anticatarral, anticonvulsivante e antitérmico. Sabendo da semelhança do cimento Portland com o MTA, e que esse ultimo apresenta bons resultados físico-químicos, surge a dúvida da possibilidade da associação, com a finalidade de melhorar as atividades antimicrobianas, e a interferência na liberação de íons cálcio e hidroxila. Objetivo: avaliar o pH, liberação de íons cálcio e atividade antimicrobiana do cimento Portland associado a Bambusa textilis em diferentes proporções. Metodologia: os cimentos experimentais foram 3 g Cimento Portland + 1,2 ml de Bambusa textilis (G1); 3 g Cimento Portland + 0,6 ml de água + 0,6 de Bambusa textilis (G2); 3 g Cimento Portland + 0,9 ml água + 0,3 ml de Bambusa textilis (G3); 3 g Cimento Portland puro (controle) + 1,2 ml água (G4); Bambusa textilis (controle): 20 microlitros por poço. Para os testes de pH e liberação de íons cálcio foram utilizados tubos de polietileno, os quais foram preenchidos com os cimentos e imersos em frascos contendo água deionizada. As mensurações foram por meio de um peagâmetro e espectrofotômetro de absorção atômica. Para a análise da atividade antimicrobiana, foi utilizada a técnica de difusão radial com cepa de Enterococcus faecalis. Os halos de inibição foram mensurados com auxílio de um paquímetro digital. A análise estatística foi realizada pelo teste ANOVA para comparação global, e teste de Tukey para comparações individuais, com significância de 5%. Resultados: os valores de pH se mostraram próximos ao neutro para todos os grupos em todos os períodos experimentais. Houve liberação de íons cálcio e ocorreu em todos os grupos e em todos os períodos experimentais. Em relação à atividade antimicrobiana, nenhuma das proporções apresentou atividade. Conclusão: a associação da Bambusa textilis ao cimento Portland manteve seu pH e liberação de íons cálcio, mas não potencializou sua atividade antimicrobiana frente ao E. faecalis. (AU)

Introduction: there is a search for phytotherapeutic agents in medicine and dentistry. Within endodontics, a substance that potentiates the biological and antimicrobial effects of calcium hydroxide as a delay dressing is sought. There are no reports in the literature about the association of Portland cement with Bambusa textilis, a plant native to China, where it is used in the manufacture of medicines, since it is an important source of resins and is therefore used for its anti-inflammatory power, anticonvulsive and antipyretic. Knowing the similarity of the Portland cement with the MTA, and that the latter presents good physicochemical results, the possibility of the association arises, with the purpose of improving the antimicrobial activities, and the interference in the release of calcium and hydroxyl ions. Aim: to analyze the pH, calcium ion release and antibacterial activity of Portland cement associated to Bambusa textilis in different proportions. Methods: the experimental cements ́ proportions were: 3 g of Portland cement + 1.2 ml of Bambusa textilis (G1); 3 g of Portland cement + 0.6 ml of water + 0.6 of Bambusa textilis (G2); 3 g of Portland cement + 0.9 ml of water + 0.3 ml of Bambusa textilis (G3); 3 g of pure Portland cement (control group) + 1.2 ml of water (G4); Bambusa textilis (control group): 20 µLper well. Polyethylene tubes filled with the cements and immersed in deionized water were employed. Calcium ion release was analyzed using an atomic absorption spectrophotometer and the change in pH was determined through a pH meter. For the analysis of antibacterial activity, a digital radial diffusion assay with a Enterococcus faecalis strain was utilized. Statistical analysis was performed using ANOVA and Tukey's tests. The level of significance was set at P = 0.05. Results: pH values were close to neutral for all of the experimental groups in all periods evaluated. Calcium ion release was observed in all experimental groups and evaluation periods. Regarding the antibacterial activity, this property could not be detected in any of the proportions used in this study. Conclusions: the association of Bambusa textilis to Portland cement, preserved its pH values and its calcium ion release property. Nevertheless, it did not improve its antibacterial activity against E. faecalis strains. (AU)

Avaliação da precisão de três localizadores foraminais na determinação do comprimento de trabalho: um estudo in vitro / Evaluation of the accuracy of three apex locators to determine the working length: an in vitro study

Um dos fatores determinantes para o sucesso do tratamento endodôntico é a determinação correta do comprimento de trabalho, pois através dele que elegemos o limite da instrumentação e da obturação evitando assim injúrias aos tecidos periapicais. Objetivo: O presente estudo teve como objetivo realizar uma análise comparativa in vitro da precisão de três localizadores foraminais, sendo eles Root ZX mini, Joypex 5 e T-Root VI. Método: Foram selecionados 30 dentes incisivos inferiores humanos extraídos. Após o acesso coronário, foi realizada a mensuração com o auxilio de um instrumento tipo K n°10 até sua ponta ser observada no forame apical com ajuda de um microscópio cirúrgico com x 50 de magnificação. Os dentes foram imersos em recipiente de plástico contendo alginato, e mensurados com os três localizadores foraminais calibrados em "Apex". Foi considerado Preciso, se a mensuração foi coincidente com comprimento real do dente; Aceitável, se a distância da medida for ate 0,5mm aquém do forame apical; e Errônea, se os valores das medidas forem menores que a medida Aceitável ou forem além do comprimento real do dente. Os dados foram submetidos aos testes de ANOVA e Tuckey (p<0,05). Resultados: Os valores observados mostraram não haver diferença estatisticamente significante (p>0.05) entre as medidas realizadas com os localizadores e o comprimento real do dente. Conclusão: Os resultados confirmam que todos dispositivos eletrônicos avaliados podem determinar com precisão o comprimento do canal radicular...

One of the most relevant factors for the success of endodontic treatment is the correct determination of the working length, since it will determine the limit of shaping and filling avoiding injuries to the periapical tissues. Objective: The aim of the present study was to compare the accuracy of three electronic apex locators: Root ZX mini, Joypex 5 and T- Root IV. Method: Thirty extracted single-rooted human incisors, extracted for reasons not related to this study, were selected and accessed. Then, the measurement of the specimens was determinated by inserting a size 10 K-file into the root canal until its tip was visible at the apical foramen by using surgical microscope at X50 magnification. The teeth were embedded in an alginate model and the analysis using the apex locators were made. The measurement restricted to 00 mm of the apical foramen (real tooth length) was classified as accurate, if the distance was determinated 0.5 mm short of the apical foramen as acceptable, and if the values of the measures were larger or smaller than the acceptable measures was classified as inaccurate. Statistical analysis was performed using ANOVA and Tuckey test (p < 0.05) Results: There were no significant differences among the three groups when electronic apex locators were used (p> 0.05). Conclusions: The results confirm that all these electronic devices can accurately determine the root canal length...