Foliar brassinosteroid analogue (DI-31) sprays increase drought tolerance by improving plant growth and photosynthetic efficiency in lulo plants.

The use of agronomic alternatives such as plant hormone sprays has been considered a tool to mitigate drought stress. This research aimed to evaluate the use of foliar brassinosteroid analogue DI-31 (BRs) sprays on plant growth, leaf exchange and chlorophyll a fluorescence parameters, and biochemical variables in lulo (Solanum quitoense L. cv. septentrionale) seedlings grown under drought stress conditions. Seedlings were grown in plastic pots (3 L) using a mix between peat and sand (1:1 v/v) as substrate. Lulo plants were subjected to drought stress by suppressing 100% of the water needs at 30-37 and 73-80 days after transplanting (DAT). Foliar BRs analogue (DI-31) sprays were carried out at four different rates (0, 1, 2, 4, or 8 mL of analogue per liter) at different times (30, 33, 44, 60, 73, and 76 DAT). Drought stress caused a reduction in the Fv/Fm ratio, leaf gas exchange properties, total biomass, and relative water content. Foliar DI-31 sprays enhanced leaf photosynthesis in well-watered (WW) (∼10.7 µmol m-2 s-1) or water-stressed plants (WS) (∼6.1 µmol m-2 s-1) when lulo plants were treated at a dose of 4 and 8 mL·L-1 compared to their respective controls (0 mL·L-1 for WW: 8.83 µmol m-2 s-1 and WS: 2.01 µmol m-2 s-1). Also, DI-31 sprays enhanced the photochemical efficiency of PSII, and plant growth. They also increased the concentration of photosynthetic pigments (TChl and Cx + c) and reduced lipid peroxidation of membranes (MDA) under drought conditions. The results allow us to suggest that the use of DI-31 at a dose of 4 or 8 mL·L-1 can be a tool for managing water stress conditions caused by low water availability in the soil in lulo-producing areas to face situations of moderate water deficit at different times of the year.

Maize Responses Challenged by Drought, Elevated Daytime Temperature and Arthropod Herbivory Stresses: A Physiological, Biochemical and Molecular View.

Maize (Zea mays L.) is one of the main cereals grown around the world. It is used for human and animal nutrition and also as biofuel. However, as a direct consequence of global climate change, increased abiotic and biotic stress events have been reported in different regions of the world, which have become a threat to world maize yields. Drought and heat are environmental stresses that influence the growth, development, and yield processes of maize crops. Plants have developed dynamic responses at the physiological, biochemical, and molecular levels that allow them to escape, avoid and/or tolerate unfavorable environmental conditions. Arthropod herbivory can generate resistance or tolerance responses in plants that are associated with inducible and constitutive defenses. Increases in the frequency and severity of abiotic stress events (drought and heat), as a consequence of climate change, can generate critical variations in plant-insect interactions. However, the behavior of herbivorous arthropods under drought scenarios is not well understood, and this kind of stress may have some positive and negative effects on arthropod populations. The simultaneous appearance of different environmental stresses and biotic factors results in very complex plant responses. In this review, recent information is provided on the physiological, biochemical, and molecular responses of plants to the combination of drought, heat stress, and the effect on some arthropod pests of interest in the maize crop.

Physiological Response of Cape Gooseberry Plants to Fusarium oxysporum f. sp. physali, Fusaric Acid, and Water Deficit in a Hydrophonic System.

Cape gooseberry production has been limited by vascular wilt caused by Fusarium oxysporum f. sp. physali (Foph). Fusaric acid (FA) is a mycotoxin produced by many Fusarium species such as F. oxysporum formae speciales. The effects of the interaction between this mycotoxin and plants (such as cape gooseberry) under biotic stress (water deficit, WD) have been little explored. Three experiments were carried out. The objectives of this study were to evaluate (i) different Foph inoculum densities (1 × 104 and 1 × 106 conidia ml-1; experiment (1); (ii) the effect of times of exposure (0, 6, 9, and 12 h) and FA concentrations (0, 12.5, 25, 50, and 100 mg L-1; experiment (2), and (iii) the interaction between Foph (1 × 104 conidia mL-1) or FA (25 mg L-1 × 9 h), and WD conditions (experiment 3) on the physiological (plant growth, leaf stomatal conductance (g s ), and photochemical efficiency of PSII (Fv/Fm ratio) and biochemical [malondialdehyde (MDA) and proline] responses of cape gooseberry seedling ecotype Colombia. The first experiment showed that Foph inoculum density of 1 × 106 conidia ml-1 caused the highest incidence of the disease (100%). In the second experiment, g s (~40.6 mmol m-2 s-1) and Fv/Fm ratio (~0.59) decreased, whereas MDA (~9.8 µmol g-1 FW) increased in plants with exposure times of 9 and 12 h and an FA concentration of 100 mg L-1 compared with plants without FA exposure or concentrations (169.8 mmol m-2 s-1, 0.8, and 7.2 µmol g-1 FW for g s , Fv/Fm ratio and MDA, respectively). In the last experiment, the interaction between Foph or FA and WD promoted a higher area under the disease progress curve (AUDPC) (Foph × WD = 44.5 and FA × WD = 37) and lower g s (Foph × WD = 6.2 mmol m-2 s-1 and FA × WD = 9.5 mmol m-2 s-1) compared with plants without any interaction. This research could be considered as a new approach for the rapid scanning of responses to the effects of FA, Foph, and WD stress not only on cape gooseberry plants but also on other species from the Solanaceae family.

Screening of Different Physalis Genotypes as Potential Rootstocks or Parents Against Vascular Wilt Using Physiological Markers.

Cape gooseberry (Physalis peruviana L.) is one of the most exported Andean fruits in Colombia. Vascular wilt caused by Fusarium oxysporum f. sp. physali (FOph) has led to a reduction in crop areas in recent years. Therefore, the aim of this study was to select genotypes with resistance to vascular wilt that can be useful as rootstocks from a group of six Physalis genotypes (Physalis ixocarpa, Physalis floridana, and Physalis peruviana genotypes Colombia, Sudafrica, Peru, and Accession 62) using physiological variables such as maximum quantum efficiency of Photosystem II (Fv/Fm), leaf gas exchange properties [net photosynthesis rate (Pn) and stomatal conductance (g s )], and leaf water potential. An experiment was carried out under greenhouse conditions in which plants of the different Physalis materials were inoculated with the F. oxysporum f. sp. physali strain Map5 at a concentration of 1 × 106 conidia mL-1. Physiological and disease development variables were measured at 15, 23, and 31 days after inoculation (DAI). The results obtained showed that P. peruviana genotypes Colombia and Sudafrica showed greater susceptibility to the disease (disease severity index 3.8 and 3.6, respectively). Net photosynthesis rate (Pn), stomatal conductance (g s ), water potential (Ψ fw ), and Fv/Fm ratio were lower compared to non-inoculated plants. P. floridana and P. ixocarpa plants inoculated with F. oxysporum showed similar behavior to non-inoculated plants for the evaluated variables. In conclusion, the results obtained suggest that these two genotypes can be considered in breeding programs or as rootstock for the establishment of cape gooseberry crops in soils with the presence of the pathogen.