RESUMO
AIMS: Loranthus tanakae Franch. & Sav is a medicinal plant that has a variety of pharmacological properties. However, its study is currently limited because of its relative shortage of natural abundance. The objective of this work was to find an alternative resource from this plant that could produce its bioactive ingredients. METHODS AND RESULTS: We isolated endophytic fungi from the twigs of Loranthus tanakae Franch. & Sav and eight flavonoid-producing endophytic fungi were selected. The eight endophytic fungi meeting the criteria were identified as Alternaria tenuissima, Dothiorella gregaria, Penicillium aethiopicum, Nothophoma quercina and Hypoxylon perforatum by morphological and molecular methods. The antioxidant and antibacterial activities of the flavonoid-producing endophytic fungi were investigated in vitro, where Alternaria tenuissima ZP28 and ZM148 demonstrated greater activities than the other six strains. Flavonoids of ZP28 and ZM148 were preliminarily identified by liquid chromatography-mass spectrometry (LC-MS). CONCLUSION: After screening the flavonoid-producing endophytic fungi, Alternaria tenuissima ZP28 and ZM148 were found to have good antioxidant and antibacterial activities. Overall, this study provided new direction and resources for the acquisition of flavonoids. SIGNIFICANCE AND IMPACT OF THE STUDY: Endophytic fungi are a promising alternative approach for the large-scale production of flavonoids from Loranthus tanakae Franch. & Sav.