RESUMO
Male BALB/c mice with streptozotocin-induced diabetes mellitus were used to study nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) damage using comet DNA assay and real-time PCR, respectively. In animals receiving single injection of streptozotocin in a dose of 200 mg/kg, severe hyperglycemia was observed on days 10 and 21 of the experiment, while after 5-fold administration of streptozotocin in a dose of 40 mg/kg, it developed on days 14 and 28. DNA damage and the level of atypical DNA comets in the liver increased both on days 10 and 21 after single administration of streptozotocin, and on days 14 and 28 after repeated administrations. The level of atypical DNA comets on day 21 after a single administration of streptozotocin increased in the kidneys, but not in the brain, testes, and pancreas. Real-time PCR revealed mtDNA damage in the liver, kidney, and pancreatic cells of mice with streptozotocin-induced diabetes. Thus, these animal models were found to reproduce pathognomic signs of diabetes, hyperglycemia, and nDNA damage; mtDNA damage was also detected.
Assuntos
Diabetes Mellitus Experimental , Hiperglicemia , Camundongos , Masculino , Animais , DNA Mitocondrial/genética , Estreptozocina , Camundongos Endogâmicos BALB C , Hiperglicemia/induzido quimicamente , Hiperglicemia/genética , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/genética , Dano ao DNARESUMO
The influence of N-acetylcysteine (ACC) on the cytogenetic effects of etoposide in F1 CBA x C57BL/6 mice was studied. Etoposide introduced intraperitoneally in doses of 10, 20, 40, and 60 mg/kg has a dose-dependent clastogenic activity and has an aneugenic effect with the induction of mainly hypohaploid oocytes. ACC significantly decreases the aneugenic and clastogenic activity of etoposide (20 mg/kg) in oocytes of 6-, 9-, and 12-week-old mice during triple introduction at a dose 200 mg/kg per os. The most pronounced anticlastogenic ACC activity (an 80% decrease) was registered in 9-week-old females; a 100% decrease in aneugenesis was detected in 6-week-old female mice.
Assuntos
Acetilcisteína/administração & dosagem , Aberrações Cromossômicas/efeitos dos fármacos , Mutagênese/efeitos dos fármacos , Oócitos/efeitos dos fármacos , Animais , Etoposídeo/toxicidade , Feminino , Camundongos , Mutagênicos/toxicidade , Oócitos/crescimento & desenvolvimentoRESUMO
We developed an original method of isolation and analysis of cytogenetic micropreparations of mouse oocytes including treatment with buffered hypotonic saline, paraformaldehyde fixation, and fluorescent staining. The method had several advantages, including high quality of sections and low labour intensity.
