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1.
Food Chem ; 456: 139971, 2024 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-38876060

RESUMO

A novel approach for simultaneous detection of iron and potassium via a smartphone-based potentiometric method is proposed in this study. The screen printed electrodes were modified with carbon black nanomaterial and ion selective membrane including zinc (II) phtalocyanine as the ionophore. The developed Fe3+-selective electrode and K+-selective electrode exhibited detection limits of 1.0 × 10-6 M and 1.0 × 10-5 M for Fe3+ and K+ ions, respectively. The electrodes were used to simultaneously detect Fe3+ and K+ ions in apple juice, skim milk, soybean and coconut water samples with recovery values between 90%-100.5%, and validated against inductively coupled plasma-optical emission spectrometry. Due to the advantageous characteristics of the sensors and the portability of Near Field Communication potentiometer supported with a smartphone application, the proposed method offers sensitive and selective detection of iron and potassium ions in food and beverage samples at the point of need.


Assuntos
Bebidas , Ferro , Potássio , Smartphone , Potássio/análise , Bebidas/análise , Ferro/análise , Potenciometria/instrumentação , Potenciometria/métodos , Leite/química , Animais , Limite de Detecção , Análise de Alimentos/instrumentação , Análise de Alimentos/métodos , Sucos de Frutas e Vegetais/análise
2.
Electrochim Acta ; 4942024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38881690

RESUMO

Laser-induced graphene (LIG) electrodes have become popular for electrochemical sensor fabrication due to their simplicity for batch production without the use of reagents. The high surface area and favorable electrocatalytic properties also enable the design of small electrochemical devices while retaining the desired electrochemical performance. In this work, we systematically investigated the effect of LIG working electrode size, from 0.8 mm to 4.0 mm diameter, on their electrochemical properties, since it has been widely assumed that the electrochemistry of LIG electrodes is independent of size above the microelectrode size regime. The background and faradaic current from cyclic voltammetry (CV) of an outer-sphere redox probe [Ru(NH3)6]3+ showed that smaller LIG electrodes had a higher electrode roughness factor and electroactive surface ratio than those of the larger electrodes. Moreover, CV of the surface-sensitive redox probes [Fe(CN)6]3- and dopamine revealed that smaller electrodes exhibited better electrocatalytic properties, with enhanced electron transfer kinetics. Scanning electron microscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy showed that the physical and chemical surface structure were different at the electrode center versus the edges, so the electrochemical properties of the smaller electrodes were improved by having rougher surface and more density of the graphitic edge planes, and more oxide-containing groups, leading to better electrochemistry. The difference could be explained by the different photothermal reaction time from the laser scribing process that causes different stable carbon morphology to form on the polymer surface. Our results give a new insight on relationships between surface structure and electrochemistry of LIG electrodes and are useful for designing miniaturized electrochemical devices.

3.
Talanta ; 276: 126211, 2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-38714009

RESUMO

A portable device offering effortlessness, mobility, and affordability for real-time and on-site monitoring of heavy metals is currently in great demand to maintain environmental sustainability. Herein, a platform utilizing a biopolymeric gel-based electrolyte for the on-field simultaneous determination of Cd(II) and Pb(II) is described. Pectin, a natural polymer, was exploited as a chemical delivery medium on account of its biodegradability, environmental friendliness, and rapid dissolving characteristics. The gel electrolyte was prepared by having pectin dissolved in KCl mixed with Sb(III)-Bi(III) bimetallic alloy solution, and casted onto a paper substrate. An in situ bimetallic alloy and pre-mixed bismuth nanoparticles modified screen-printed graphene electrode (Sb-Bi/BiNP/SPGE) were employed to enhance the electrochemical signals of Cd(II) and Pb(II) for the differential pulse anodic stripping voltammetry (DPASV). It was demonstrated that the platform was capable of generating sharp and well-defined current signals, achieving the low detection limits of 50.98 ng mL-1 for Cd(II) and 40.80 ng mL-1 for Pb(II). The reproducibility, as indicated by the relative standard deviation, was found to be less than 10.4 % (n = 10) for the developed gel-based device when coupled with a wireless near field communication (NFC) potentiostat. Lastly, the obtained sensor was applied for quantification of Cd and Pb in potentially contaminated groundwater samples. The recoveries obtained were satisfactorily within the acceptable range. The newly designed platform exhibited several advantages, including small sample volume (µL), low-cost, no sample preparation requirements, and being environmentally friendly. The convenience of a portable device utilizing the proposed biopolymeric gel-based electrolyte for on-field analysis makes it highly appealing for various applications.

4.
Curr Top Med Chem ; 24(11): 986-1009, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38584544

RESUMO

This review explores the advancements in nanomaterial-based electrochemical sensors for the multiplex detection of medicinal compounds. The growing demand for efficient and selective detection methods in the pharmaceutical field has prompted significant research into the development of electrochemical sensors employing nanomaterials. These materials, defined as functional materials with at least one dimension between 1 and 100 nanometers, encompass metal nanoparticles, polymers, carbon-based nanocomposites, and nano-bioprobes. These sensors are characterized by their enhanced sensitivity and selectivity, playing a crucial role in simultaneous detection and offering a comprehensive analysis of multiple medicinal complexes within a single sample. The review comprehensively examines the design, fabrication, and application of nanomaterial- based electrochemical sensors, focusing on their ability to achieve multiplex detection of various medicinal substances. Insights into the strategies and nanomaterials employed for enhancing sensor performance are discussed. Additionally, the review explores the challenges and future perspectives of this evolving field, highlighting the potential impact of nanomaterial-based electrochemical sensors on the advancement of medicinal detection technologies.


Assuntos
Técnicas Eletroquímicas , Nanoestruturas , Nanoestruturas/química , Humanos , Técnicas Biossensoriais , Preparações Farmacêuticas/análise , Preparações Farmacêuticas/química
5.
Biosens Bioelectron ; 250: 116079, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38295580

RESUMO

Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.


Assuntos
Técnicas Biossensoriais , COVID-19 , Humanos , SARS-CoV-2/genética , COVID-19/diagnóstico , Pandemias , Eletrodos , DNA/genética , Ouro/química , Técnicas Eletroquímicas
6.
Anal Chem ; 95(34): 12794-12801, 2023 08 29.
Artigo em Inglês | MEDLINE | ID: mdl-37590190

RESUMO

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a significant health issue globally. Point-of-care (POC) testing that can offer a rapid and accurate diagnosis of SARS-CoV-2 at the early stage of infection is highly desirable to constrain this outbreak, especially in resource-limited settings. Herein, we present a G-quadruplex DNAzyme-based electrochemical assay that is integrated with a sequential flow controllable microfluidic device for the detection of SARS-CoV-2 cDNA. According to the detection principle, a pyrrolidinyl peptide nucleic acid probe is immobilized on a screen-printed graphene electrode for capturing SARS-CoV-2 DNA. The captured DNA subsequently hybridizes with another DNA probe that carries a G-quadruplex DNAzyme as the signaling unit. The G-quadruplex DNAzyme catalyzes the H2O2-mediated oxidation of hydroquinone to benzoquinone that can be detected using square-wave voltammetry to give a signal that corresponds to the target DNA concentration. The assay exhibited high selectivity for SARS-CoV-2 DNA and showed a good experimental detection limit at 30 pM. To enable automation, the DNAzyme-based assay was combined with a capillary-driven microfluidic device featuring a burst valve technology to allow sequential sample and reagent delivery as well as the DNA target hybridization and enzymatic reaction to be operated in a precisely controlled fashion. The developed microfluidic device was successfully applied for the detection of SARS-CoV-2 from nasopharyngeal swab samples. The results were in good agreement with the standard RT-PCR method and could be performed within 20 min. Thus, this platform offers desirable characteristics that make it an alternative POC tool for COVID-19 diagnosis.


Assuntos
COVID-19 , DNA Catalítico , Ácidos Nucleicos Peptídicos , Humanos , SARS-CoV-2 , COVID-19/diagnóstico , Teste para COVID-19 , Peróxido de Hidrogênio
7.
Mikrochim Acta ; 190(9): 362, 2023 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-37608141

RESUMO

Sepsis, an infectious disease affecting millions of people's health worldwide each year, calls for urgent attention to an improvement of analytical devices. Chemiluminescence immunoassay is a typical diagnostic method utilized to assess the risk development of sepsis. However, due to its high-cost, delayed, and complicated procedure, the practical utilization is therefore undoubtedly limited, especially for point-of-care test. Herein, we fabricated for the first time an immunosensor based on dendritic copper nanostructures (CuNSs) combined with 4-aminobenzoic acid (4-AB, the diazonium salt) as antibody linker modified on a screen-printed graphene electrode for the early detection of the sepsis biomarker interleukin-6 (IL-6). The electrode fabrication is made by electrodeposition, thus eliminating the multistep of nanomaterial synthesis and time wasting. The resulting dendritic CuNSs significantly increase the effective surface area (1.2 times) and the sensor's performance. The morphology of this combination was characterized using CV, EIS, SEM, EDX, and FTIR techniques. In the detection process, the appearance of IL-6 suppresses the current response of the redox probe indicator measured by differential pulse voltammetry due to the antibody-antigen complex. The subtraction of signal (ΔI) was interpreted as IL-6 concentration. This sensor exhibited a linear range from 0.05 to 500 pg mL-1 with low detection limit of 0.02 pg mL-1, proving a possibility for early sepsis screening. In addition, the established immunosensor can successfully quantify IL-6 in human serum sample, in which the results agreed well with those achieved using the standard approach, further showing high practical applicability of this developed immunosensor.


Assuntos
Técnicas Biossensoriais , Grafite , Sepse , Humanos , Interleucina-6 , Cobre , Imunoensaio , Sepse/diagnóstico , Eletrodos
8.
Talanta ; 265: 124906, 2023 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-37451117

RESUMO

An automated microfluidic electrochemical platform was developed for the rapid in-field analysis of arsenic speciation. Herein, we integrated an electrochemical sensing and microfluidic channel for the simultaneous determination of As(III) and total inorganic As (total iAs) within a single device. The platform was fabricated by assembling a gold nanoparticle-modified screen-printed graphene electrode (AuNP/SPGE) on a hydrophilic polyethylene terephthalate (PET) sheet that was specially designed to enclose a microfluidic channel with dual flow channels for separate determination of the two species. While As(III) can be promptly detected with the AuNP/SPGE on one end, thioglycolic acid stored in glass fiber is employed on the other end to reduce As(V) before being electrochemically analyzed on the AuNP/SPGE as total iAs; the difference represents the amount of As(V). With a wireless potentiostat and a smartphone equipped with Bluetooth technology, the overall procedure can be fully automated and accomplished merely within 9 min. The linear ranges for the determination of As(III) and total iAs were found to be 50-1000 and 100-1500 ng/mL with detection limits of 3.7 and 17 ng/mL, respectively. The proposed method was validated and applied for the inorganic As speciation of various food samples with satisfactory results compared to those obtained with the standard HPLC-ICP‒MS protocol. This novel microfluidic electrochemical platform offers numerous advantages, notably for its simplicity, speed, low cost, and portability for on-site analysis, which conclusively makes it a highly promising analytical device for the speciation of inorganic arsenic.

9.
ADMET DMPK ; 11(2): 303-315, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37325121

RESUMO

In this work, an automated flow injection analysis (FIA) connected to a boron-doped diamond electrode (BDDE) was originally developed for the analysis of methimazole in pharmaceutical preparations. At a modification-free BDDE, methimazole was easilly oxidized. For the analysis of the mechanisms occurring at the electrode surface, cyclic voltammetry was employed to evaluate the impact of fundamental experimental parameters, such as pH and scan rate, on the BDDE response. For the quantitative detection, the FIA amperometric approach was constructed and used as a fast and sensitive method. The suggested approach provided a broad linear range of 0.5-50 µmol/L and a low detection limit of 10 nmol/L (signal-to-noise ratio = 3). Furthermore, the BDDE was successfully utilized to quantify methimazole in genuine samples from a variety of medicines, and its performance remained steady after more than 50 tests. The findings of amperometric measurements exhibit excellent repeatability, with relative standard deviations of less than 3.9 and 4.7 % for intra-day and inter-day, respectively. The findings indicated that, compared with traditional approaches, the suggested method has the following advantages: quick analysis time, simplicity, highly sensitive output, and no need for complicated operational processes.

10.
Analyst ; 148(13): 3107-3116, 2023 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-37313729

RESUMO

Amino acid conductive polymers can easily form a thin film on a sensor surface by an electrochemical process. Therefore, we are pioneers in reporting the electropolymerization of L-methionine on the surface of a screen-printed graphene electrode to obtain a disposable electrochemical sensor for determining drug metabolites (5-aminosalicylic acid (5-ASA) and sulfapyridine (SPD)) of sulfasalazine (SSZ) simultaneously. In this work, the developed sensor was facilely created through a single step of electropolymerization under mild conditions (0.1 M phosphate buffer pH 7.0) using cyclic voltammetry. Important parameters in the synthesis process were systematically investigated followed by surface composition and morphology studies. Then, analytical performances, comprising sensitivity, selectivity, stability, reproducibility, and sample preparation, were carefully evaluated. Under optimal conditions, the proposed methodology demonstrated a highly sensitive and selective simultaneous detection of 5-ASA and SPD with wide linear dynamic ranges of 1-50 µM and 80-250 µM and low detection limits of 0.60 and 0.57 µM for 5-ASA and SPD, respectively. To evaluate the potential of the designed sensor, it was successfully applied by simultaneously determining 5-ASA and SPD in real human urine samples on the same day (intra-day study) and on three different days (inter-day study).


Assuntos
Grafite , Mesalamina , Humanos , Sulfapiridina , Polímeros/química , Reprodutibilidade dos Testes , Eletrodos , Grafite/química , Técnicas Eletroquímicas/métodos , Limite de Detecção
11.
Mikrochim Acta ; 190(6): 237, 2023 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-37222781

RESUMO

A significant bottleneck exists for mass-production of ion-selective electrodes despite recent developments in manufacturing technologies. Here, we present a fully-automated system for large-scale production of ISEs. Three materials, including polyvinyl chloride, polyethylene terephthalate and polyimide, were used as substrates for fabricating ion-selective electrodes (ISEs) using stencil printing, screen-printing and laser engraving, respectively. We compared sensitivities of the ISEs to determine the best material for the fabrication process of the ISEs. The electrode surfaces were modified with various carbon nanomaterials including multi-walled carbon nanotubes, graphene, carbon black, and their mixed suspensions as the intermediate layer to enhance sensitivities of the electrodes. An automated 3D-printed robot was used for the drop-cast procedure during ISE fabrication to eliminate manual steps. The sensor array was optimized, and the detection limits were 10-5 M, 10-5 M and 10-4 M for detection of K+, Na+ and Ca2+ ions, respectively. The sensor array integrated with a portable wireless potentiometer was used to detect K+, Na+ and Ca2+ in real urine and simulated sweat samples and results obtained were in agreement with ICP-OES with good recoveries. The developed sensing platform offers low-cost detection of electrolytes for point-of-care applications.


Assuntos
Líquidos Corporais , Nanotubos de Carbono , Eletrodos Seletivos de Íons , Smartphone , Íons
12.
Anal Chim Acta ; 1265: 341257, 2023 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-37230584

RESUMO

Electrochemical DNA sensors can be operated in either static or flow-based detection schemes. In static schemes, manual washing steps are still necessary, resulting in a tedious and time-consuming process. In contrast, in flow-based electrochemical sensors, the current response is collected when the solution flows through the electrode continuously. However, the drawback of such a flow system is the low sensitivity due to the limited time for the interaction between the capturing element and the target. Herein, we propose a novel electrochemical capillary-driven microfluidic DNA sensor to combine the advantages of static and flow-based electrochemical detection systems into a single device by incorporating burst valve technology. The microfluidic device with a two-electrode configuration was applied for the simultaneous detection of two different DNA markers, human immunodeficiency virus-1 (HIV-1) and hepatitis C virus (HCV) cDNA, via the specific interaction between pyrrolidinyl peptide nucleic acids (PNA) probes and the DNA target. The integrated system, while requiring a small sample volume (7 µL for each sample loading port) and less analysis time, achieved good performance in terms of the limits of detection (LOD) (3SDblank/slope) and quantification (LOQ) (10SDblank/slope) at 1.45 nM and 4.79 nM for HIV and 1.20 nM and 3.96 nM for HCV, respectively. The simultaneous detection of HIV-1 and HCV cDNA prepared from human blood samples showed results that are in complete agreement with the RT‒PCR assay. The results qualify this platform as a promising alternative for the analysis of either HIV-1/HCV or coinfection that can be easily adapted for other clinically important nucleic acid-based markers.


Assuntos
Coinfecção , Infecções por HIV , HIV-1 , Hepatite C , Humanos , Hepacivirus/genética , Microfluídica , HIV-1/genética , DNA Complementar , DNA , Hepatite C/diagnóstico , Infecções por HIV/diagnóstico
13.
Anal Methods ; 15(22): 2721-2728, 2023 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-37099406

RESUMO

A capillary-driven microfluidic sequential flow device, designed for eventual at-home or doctor's office use, was developed to perform an enzyme-linked immunosorbent assay (ELISA) for serology assays. Serology assays that detect SARS-CoV-2 antibodies can be used to determine prior infection, immunity status, and/or individual vaccination status and are typically run using well-plate ELISAs in centralized laboratories, but in this format SARs-CoV-2 serology tests are too expensive and/or slow for most situations. Instead, a point-of-need device that can be used at home or in doctor's offices for COVID-19 serology testing would provide critical information for managing infections and determining immune status. Lateral flow assays are common and easy to use, but lack the sensitivity needed to reliably detect SARS-CoV-2 antibodies in clinical samples. This work describes a microfluidic sequential flow device that is as simple to use as a lateral flow assay, but as sensitive as a well-plate ELISA through sequential delivery of reagents to the detection area using only capillary flow. The device utilizes a network of microfluidic channels made of transparency film and double-sided adhesive combined with paper pumps to drive flow. The geometry of the channels and storage pads enables automated sequential washing and reagent addition steps with two simple end-user steps. An enzyme label and colorimetric substrate produce an amplified, visible signal for increased sensitivity, while the integrated washing steps decrease false positives and increase reproducibility. Naked-eye detection can be used for qualitative results or a smartphone camera for quantitative analysis. The device detected antibodies at 2.8 ng mL-1 from whole blood, while a well-plate ELISA using the same capture and detection antibodies could detect 1.2 ng mL-1. The performance of the capillary-driven immunoassay (CaDI) system developed here was confirmed by demonstrating SARS-CoV-2 antibody detection, and we believe that the device represents a fundamental step forward in equipment-free point-of-care technology.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , COVID-19/diagnóstico , Microfluídica , Reprodutibilidade dos Testes , Ensaio de Imunoadsorção Enzimática/métodos , Anticorpos Antivirais
14.
Sci Rep ; 13(1): 3523, 2023 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-36864072

RESUMO

A wireless-based detection utilizing an innovative electrochemical card (eCard) sensor controlled by a smartphone was developed for targeting Hepatitis B surface antigen (HBsAg). A simple label-free electrochemical platform allows a convenient operation for point-of-care diagnosis. A disposable screen-printed carbon electrode was modified straightforwardly layer-by-layer with chitosan followed by glutaraldehyde, allowing a simple but effective, reproducible, and stable method for covalently immobilizing antibodies. The modification and immobilization processes were verified by electrochemical impedance spectroscopy and cyclic voltammetry. The smartphone-based eCard sensor was used to quantify HBsAg by measuring the change in current response of the [Fe(CN)6]3-/4- redox couple before and after the presence of HBsAg. Under the optimal conditions, the linear calibration curve for HBsAg was found to be 10-100,000 IU/mL with a detection limit of 9.55 IU/mL. The HBsAg eCard sensor was successfully applied to detect 500 chronic HBV-infected serum samples with satisfactory results, demonstrating the excellent applicability of this system. The sensitivity and specificity of this sensing platform were found to be 97.75% and 93%, respectively. As illustrated, the proposed eCard immunosensor offered a rapid, sensitive, selective, and easy-to-use platform for healthcare providers to rapidly determine the infection status of HBV patients.


Assuntos
Técnicas Biossensoriais , Humanos , Antígenos de Superfície da Hepatite B , Imunoensaio , Anticorpos , Calibragem
15.
Biosens Bioelectron ; 221: 114352, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-35690559

RESUMO

Nucleic acid biosensors for point-of-care (POC) diagnostic applications are highly desirable. The ability to detect DNA and RNA in a simple, rapid, affordable and portable format leads to a range of important applications for early screening in the field of disease monitoring and management. Herein, we report the development of an isothermal, label-free electrochemical biosensor that was designed on the basis of target-driven MNAzyme cleavage activity. Hybridization with HPV mRNA, a model nucleic acid target, activated MNAzyme and initiated the cleavage of immobilized hairpin substrates, leading to changes in the electrochemical signal. Under optimal conditions, a detection limit of 2.6 pM was obtained with an incubation time of 60 min. Furthermore, an artificial chaperone-enhanced MNAzyme (ACEzyme) system was integrated to an electrochemical biosensor for the first time. The analytical performance of the biosensor was enhanced, and the detection time was significantly reduced by the addition of PLL-g-Dex, which exhibits nucleic acid chaperone-like activity. A detection limit of 0.88 pM was obtained with a threefold decrease in incubation time without prior amplification. The proposed biosensing platform shows the advantages of simple fabrication and operation, good selectivity in the presence of single-base mismatch, and excellent versatility in a complex mixture of total RNA. We believe that this isothermal, label-free, and protein-free nucleic acid analysis platform could provide foundations for the further development of a universal nucleic acid biosensing platform for clinical application.


Assuntos
Técnicas Biossensoriais , Infecções por Papillomavirus , Humanos , Técnicas Eletroquímicas , RNA Mensageiro/genética , RNA , Limite de Detecção , Técnicas de Amplificação de Ácido Nucleico
16.
Talanta ; 254: 124131, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36470021

RESUMO

3D printing technologies are an attractive for fabricating electrochemical sensors due to their ease of operation, freedom of design, fast prototyping, low waste, and low cost. We report the fabrication of a simple 3D-printed electrochemical sensing device for non-enzymatic detection of creatinine, an important indicator of renal function. To create the 3D-printed electrodes (3DE), carbon black/polylactic acid (CB/PLA) composite filament was used. The 3DE was activated using 0.5 M NaOH via amperometry prior to use to improve electrochemical performance. To give selectivity for creatinine, the activated 3DE was modified with a copper oxide nanoparticle-ionic liquid/reduced graphene oxide (CuO-IL/rGO) composite. The modified 3DE was characterized using microscopy and electrochemistry. Cyclic voltammetry and amperometry were used to evaluate sensor performance. The modified 3DE provided electrocatalytic activity towards creatinine without enzymes. Under optimal conditions, the modified 3DE directly coupled with a portable smartphone potentiostat exhibited the linear detection range of 0.5-35.0 mM, and the limit of detection was 37.3 µM, which is sufficient for detecting creatinine in human urine samples. Furthermore, the other physiological compounds present in human urine were not detected on the modified 3DE. Therefore, the modified 3DE could be a tool for effective creatinine screening in the urine.


Assuntos
Grafite , Nanopartículas , Humanos , Creatinina/química , Limite de Detecção , Smartphone , Técnicas Eletroquímicas , Grafite/química , Nanopartículas/química , Eletrodos
17.
Talanta ; 253: 123992, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36228554

RESUMO

The COVID-19 pandemic has significantly increased the development of the development of point-of-care (POC) diagnostic tools because they can serve as useful tools for detecting and controlling spread of the disease. Most current methods require sophisticated laboratory instruments and specialists to provide reliable, cost-effective, specific, and sensitive POC testing for COVID-19 diagnosis. Here, a smartphone-assisted Sensit Smart potentiostat (PalmSens) was integrated with a paper-based electrochemical sensor to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A disposable paper-based device was fabricated, and the working electrode directly modified with a pyrrolidinyl peptide nucleic acid (acpcPNA) as the biological recognition element to capture the target complementary DNA (cDNA). In the presence of the target cDNA, hybridization with acpcPNA probe blocks the redox conversion of a redox reporter, leading to a decrease in electrochemical response correlating to SARS-CoV-2 concentration. Under optimal conditions, a linear range from 0.1 to 200 nM and a detection limit of 1.0 pM were obtained. The PNA-based electrochemical paper-based analytical device (PNA-based ePAD) offers high specificity toward SARS-CoV-2 N gene because of the highly selective PNA-DNA binding. The developed sensor was used for amplification-free SARS-CoV-2 detection in 10 nasopharyngeal swab samples (7 SARS-CoV-2 positive and 3 SARS-CoV-2 negative), giving a 100% agreement result with RT-PCR.


Assuntos
COVID-19 , Humanos , COVID-19/diagnóstico , SARS-CoV-2/genética , Teste para COVID-19 , Pandemias , DNA
18.
Talanta ; 251: 123783, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35977451

RESUMO

The current approaches of diagnostic platforms for detecting SARS-CoV-2 infections mostly relied on adapting the existing technology. In this work, a simple and low-cost electrochemical sensing platform for detecting SAR-CoV-2 antigen was established. The proposed sensor combined the innovative disposable paper-based immunosensor and cost-effective plant-based anti-SARS-CoV-2 monoclonal antibody CR3022, expressed in Nicotiana benthamiana. The cellulose nanocrystal was modified on screen-printed graphene electrode to provide the abundant COOH functional groups on electrode surface, leading to the high ability for antibody immobilization. The quantification of the presence receptor binding domain (RBD) spike protein of SARS-CoV-2 was performed using differential pulse voltammetry by monitoring the changing current of [Fe(CN)6]3-/4- redox solution. The current change of [Fe(CN)6]3-/4- before and after the presence of target RBD could be clearly distinguished, providing a linear relationship with RBD concentration in the range from 0.1 pg/mL to 500 ng/mL with the minimum limit of detection of 2.0 fg/mL. The proposed platform was successfully applied to detect RBD in nasopharyngeal swab samples with satisfactory results. Furthermore, the paper-based immunosensor was extended to quantify the RBD level in spiked saliva samples, demonstrating the broadly applicability of this system. This electrochemical paper-based immunosensor has the potential to be employed as a point-of-care testing for COVID-19 diagnosis.


Assuntos
Técnicas Biossensoriais , COVID-19 , Grafite , Anticorpos Monoclonais/química , Anticorpos Neutralizantes , Anticorpos Antivirais , Técnicas Biossensoriais/métodos , COVID-19/diagnóstico , Teste para COVID-19 , Celulose , Técnicas Eletroquímicas/métodos , Grafite/química , Humanos , Imunoensaio/métodos , SARS-CoV-2 , Glicoproteína da Espícula de Coronavírus
19.
Sci Rep ; 12(1): 19866, 2022 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-36400826

RESUMO

Cardiac troponin I (cTnI) is a specific cardiac biomarker for diagnosis of acute myocardial infarction (AMI). A sensitive and simple point-of-care test (POCT) is still required for early detection of AMI. To address this need, we developed a dip strip assay based on sandwich immunoassay coupled with a silver enhancement system. Pre-incubation and silver enhancement were introduced to the dip strip to increase sensitivity. Due to the catalytic reaction of the silver enhancement solution, the red color of AuNPs changed to dark brown as silver ions precipitated and enlarged the AuNPs. The obtained results were easily seen by the naked eye. For quantitative analysis, the color intensity of the results was analyzed using a smartphone with RGB color picker application. The effects of operating parameters (volume of AuNP-Ab conjugate, volume of sample, incubation time, and analysis time) were investigated and optimized. Under optimal conditions, the limit of detection (LOD) by the naked eye was 0.5 ng/mL. The LOD with silver enhancement was 50-fold lower than without. For quantitative analysis using the smartphone, linearity of detection was observed through the range of 0.5-50 ng/mL (R2 = 0.9952) and the LOD was 0.12 ng/mL. The developed method was successfully applied to detection of cTnI in serum samples, achieving analytical recoveries and %RSD in the ranges of 96.10-119.17% and 2.91-5.13%, respectively. Additionally, this developed assay was not cross reactive with the potentially interfering serum proteins. These results showed the great potential of this dip strip assay as an alternative POCT for detection of serum cTnI.


Assuntos
Nanopartículas Metálicas , Infarto do Miocárdio , Humanos , Troponina I , Prata , Coloide de Ouro , Colorimetria/métodos , Smartphone , Ouro , Imunoensaio/métodos , Biomarcadores , Infarto do Miocárdio/diagnóstico
20.
Curr Top Med Chem ; 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36330618

RESUMO

BACKGROUND: The demand for point-of-care testing (POCT) devices has rapidly grown since they offer immediate test results with ease of use, makingthem suitable for home self-testing patients and caretakers. However, the POCT development has faced the challenges of increased cost and limited resources. Therefore, the paper substrate as a low-cost material has been employed to develop a cost-effective POCT device, known as "Microfluidic paper-based analytical devices (µPADs)". This device is gaining attention as a promising tool for medicinal diagnostic applications owing to its unique features of simple fabrication, low cost, enabling manipulation flow (capillarydriven flow), the ability to store reagents, and accommodating multistep assay requirements. OBJECTIVE: This review comprehensively examines the fabrication methods and device designs (2D/3D configuration) and their advantages and disadvantages, focusing on updated µPADs applications for motif identification. METHODS: The evolution of paper-based devices, starting from the traditional devices of dipstick and lateral flow assay (LFA) with µPADs, has been described. Patterned structure fabrication of each technique has been compared among the equipment used, benefits, and drawbacks. Microfluidic device designs, including 2D and 3D configurations, have been introduced as well as their modifications. Various designs of µPADs have been integrated with many powerful detection methods such as colorimetry, electrochemistry, fluorescence, chemiluminescence, electrochemiluminescence, and SER-based sensors for medicinal diagnosis applications. CONCLUSION: The µPADs potential to deal with commercialization in terms of the state-of-the-art of µPADs in medicinal diagnosis has been discussed. A great prototype, which is currently in a reallife application breakthrough, has been updated.

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