Impact of systemic hypoxia and blood flow restriction on mechanical, cardiorespiratory, and neuromuscular responses to a multiple-set repeated sprint exercise.

Introduction: Repeated sprint cycling exercises (RSE) performed under systemic normobaric hypoxia (HYP) or with blood flow restriction (BFR) are of growing interest. To the best of our knowledge, there is no stringent consensus on the cardiorespiratory and neuromuscular responses between systemic HYP and BFR during RSE. Thus, this study assessed cardiorespiratory and neuromuscular responses to multiple sets of RSE under HYP or with BFR. Methods: According to a crossover design, fifteen men completed RSE (three sets of five 10-s sprints with 20 s of recovery) in normoxia (NOR), HYP, and with bilaterally-cuffed BFR at 45% of resting arterial occlusive pressure during sets in NOR. Power output, cardiorespiratory and neuromuscular responses were assessed. Results: Average peak and mean powers were lower in BFR (dz = 0.87 and dz = 1.23, respectively) and HYP (dz = 0.65 and dz = 1.21, respectively) compared to NOR (p < 0.001). The percentage decrement of power output was greater in BFR (dz = 0.94) and HYP (dz = 0.64) compared to NOR (p < 0.001), as well as in BFR compared to NOR (p = 0.037, dz = 0.30). The percentage decrease of maximal voluntary contraction of the knee extensors after the session was greater in BFR compared to NOR and HYP (p = 0.011, dz = 0.78 and p = 0.027, dz = 0.75, respectively). Accumulated ventilation during exercise was higher in HYP and lower in BFR (p = 0.002, dz = 0.51, and p < 0.001, dz = 0.71, respectively). Peak oxygen consumption was reduced in HYP (p < 0.001, dz = 1.47). Heart rate was lower in BFR during exercise and recovery (p < 0.001, dz = 0.82 and p = 0.012, dz = 0.43, respectively). Finally, aerobic contribution was reduced in HYP compared to NOR (p = 0.002, dz = 0.46) and BFR (p = 0.005, dz = 0.33). Discussion: Thus, this study indicates that power output during RSE is impaired in HYP and BFR and that BFR amplifies neuromuscular fatigue. In contrast, HYP did not impair neuromuscular function but enhanced the ventilatory response along with reduced oxygen consumption.

Specific Incremental Test for Aerobic Fitness in Trail Running: IncremenTrail.

Trail running (TR) is performed in a natural environment, including various ranges of slopes where maximal oxygen consumption is a major contributor to performance. The aim of this study is to investigate the validity of tests performed in uphill conditions named the "IncremenTrail" (IncT), based on the incremental ascending speed (AS) to evaluate trail runners' cardiorespiratory parameters. IncT protocol included a constant gradient slope set at 25% during the whole test; the starting speed was 500 m·h-1 (25% slope and 2.06 km·h-1) and increased by 100 m·h-1 every minute (0.41 km·h-1). Twenty trail runner specialists performed the IncT and a supramaximal exercise bout to exhaustion with intensity set at 105% of maximal AS (Tlim). Oxygen consumption, breathing frequency, ventilation, respiratory exchange ratio (RER), and heart rate were continuously recorded during the exercises. The blood lactate concentration and rate of perceived exertion were collected at the end of the exercises. During the IncT test, 16 athletes (80%) reached a plateau of maximal oxygen uptake (65.5 ± 7.6 mL·kg-1·min-1), 19 athletes (95%) reached RER values over 1.10 (1.12 ± 0.02) and all the athletes achieved blood lactate concentration over 8.0 mmol·L-1 (17.1 ± 3.5 mmol·L-1) and a maximal heart rate ≥90% of the theoretical maximum (185 ± 11 bpm). Maximal values were not significantly different between IncT and Tlim. In addition, ventilatory thresholds could be determined for all runners with an associated AS. IncT provided a suitable protocol to evaluate trail runners' cardiorespiratory limitations and allowed us to obtain specific intensities based on the ascending speed useful for training purposes in specific conditions.

Physiological Implication of Slope Gradient during Incremental Running Test.

Uphill running induces a higher physiological demand than level conditions. Although many studies have investigated this locomotion from a psychological point of view, there is no clear position on the effects of the slope on the physiological variables during an incremental running test performed on a slope condition. The existing studies have heterogeneous designs with different populations or slopes and have reported unclear results. Some studies observed an increase in oxygen consumption, whereas it remained unaffected in others. The aim of this study is to investigate the effect of a slope on the oxygen consumption, breathing frequency, ventilation and heart rate during an incremental test performed on 0, 15, 25 and 40% gradient slopes by specialist trail runners. The values are compared at the first and second ventilatory threshold and exhaustion. A one-way repeated measures ANOVA, with a Bonferroni post-hoc analysis, was used to determine the effects of a slope gradient (0, 15, 25 and 40%) on the physiological variables. Our study shows that all the variables are not affected in same way by the slopes during the incremental test. The heart rate and breathing frequency did not differ from the level condition and all the slope gradients at the ventilatory thresholds or exhaustion. At the same time, the ventilation and oxygen consumption increased concomitantly with the slope (p < 0.001) in all positions. The post-hoc analysis highlighted that the ventilation significantly increased between each successive gradient (0 to 15%, 15% to 25% and 25% to 40%), while the oxygen consumption stopped increasing at the 25% gradient. Our results show that the 25 and 40% gradient slopes allow the specialist trail runners to reach the highest oxygen consumption level.