Assuntos
Vacinas contra Influenza/uso terapêutico , Influenza Humana/epidemiologia , Influenza Humana/prevenção & controle , Vacinação/estatística & dados numéricos , Criança , Pré-Escolar , Feminino , Hong Kong/epidemiologia , Humanos , Vírus da Influenza A/genética , Modelos Logísticos , Masculino , Estudos ProspectivosAssuntos
Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/fisiopatologia , Influenza Humana/virologia , Nasofaringe/virologia , Pré-Escolar , Exposição Ambiental , Feminino , Hong Kong , Humanos , Vírus da Influenza A Subtipo H1N1/classificação , Vacinas contra Influenza/uso terapêutico , Influenza Humana/prevenção & controle , Masculino , Reação em Cadeia da Polimerase Multiplex , Vigilância da População , Estudos ProspectivosAssuntos
Genes p53 , Mutação , Neoplasias Orofaríngeas/etiologia , Infecções por Papillomavirus/complicações , Fumar/efeitos adversos , Carcinoma de Células Escamosas de Cabeça e Pescoço/etiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Consumo de Bebidas Alcoólicas/efeitos adversos , Estudos de Casos e Controles , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Orofaríngeas/genética , Fatores de RiscoRESUMO
BACKGROUND: Polymorphisms of CLEC4M have been associated with predisposition for infection by the severe acute respiratory syndrome coronavirus (SARS-CoV). DC-SIGNR, a C-type lectin encoded by CLEC4M, is a receptor for the virus. A variable number tandem repeat (VNTR) polymorphism in its neck region was recently associated with susceptibility to SARS infection. However, this association was controversial and was not supported by subsequent studies. Two explanations may account for this discrepancy: (1) there may be an unknown predisposition polymorphism located in the proximity which is linked to the VNTR; or (2) it was a spurious association due to unrecognised population structure in the VNTR. METHODS: We performed a comprehensively genetic association study on this C-type lectin gene cluster (FCER2, CLEC4G, CD209, and CLEC4M) at 19p13.3 by a tagging single nucleotide polymorphisms (SNPs) approach. RESULTS: 23 tagSNPs were genotyped in 181 SARS patients and 172 population controls. No significant association with disease predisposition was detected. Genetic variations in this cluster also did not predict disease prognosis. However, we detected a population stratification of the VNTR alleles in a sample of 1145 Han Chinese collected from different parts of China. CONCLUSION: The results indicated that the genetic predisposition allele was not found in this lectin gene cluster and population stratification might cause the previous positive association.
Assuntos
Cromossomos Humanos Par 19/genética , Predisposição Genética para Doença , Lectinas Tipo C/genética , Família Multigênica , Polimorfismo de Nucleotídeo Único/genética , Síndrome Respiratória Aguda Grave/genética , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave/patogenicidade , Moléculas de Adesão Celular/genética , China/epidemiologia , China/etnologia , Genótipo , Humanos , Repetições Minissatélites/genética , Receptores de Superfície Celular/genética , Síndrome Respiratória Aguda Grave/epidemiologia , Síndrome Respiratória Aguda Grave/etnologia , Adulto JovemRESUMO
BACKGROUND: Severe acute respiratory syndrome (SARS) became a worldwide outbreak with a mortality of 9.2%. This new human emergent infectious disease is dominated by severe lower respiratory illness and is aetiologically linked to a new coronavirus (SARS-CoV). METHODS: Pulmonary pathology and clinical correlates were investigated in seven patients who died of SARS in whom there was a strong epidemiological link. Investigations include a review of clinical features, morphological assessment, histochemical and immunohistochemical stainings, ultrastructural study, and virological investigations in postmortem tissue. RESULTS: Positive viral culture for coronavirus was detected in most premortem nasopharyngeal aspirate specimens (five of six) and postmortem lung tissues (two of seven). Viral particles, consistent with coronavirus, could be detected in lung pneumocytes in most of the patients. These features suggested that pneumocytes are probably the primary target of infection. The pathological features were dominated by diffuse alveolar damage, with the presence of multinucleated pneumocytes. Fibrogranulation tissue proliferation in small airways and airspaces (bronchiolitis obliterans organising pneumonia-like lesions) in subpleural locations was also seen in some patients. CONCLUSIONS: Viable SARS-CoV could be isolated from postmortem tissues. Postmortem examination allows tissue to be sampled for virological investigations and ultrastructural examination, and when coupled with the appropriate lung morphological changes, is valuable to confirm the diagnosis of SARS-CoV, particularly in clinically unapparent or suspicious but unconfirmed cases.
Assuntos
Coronavirus/isolamento & purificação , Pulmão/patologia , Síndrome Respiratória Aguda Grave/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Divisão Celular , Núcleo Celular/patologia , Células Cultivadas , Feminino , Humanos , Imuno-Histoquímica/métodos , Pulmão/virologia , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Alvéolos Pulmonares/patologia , Síndrome Respiratória Aguda Grave/virologiaRESUMO
We describe a case of asymptomatic human parvovirus B19 infection during pregnancy that led to hydrops fetalis and foetal death. The diagnosis was confirmed by the presence of parvovirus B19-specific IgM antibodies in the maternal serum and the pathognomonic findings, which showed intranuclear eosinophilic viral inclusions and margination of chromatin in foetal cells of haemopoietic lineage. Details of clinical, virological, and histological findings are presented. Difficulties in confirming the diagnosis of parvovirus B19 infection in Hong Kong are discussed.