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IEEE Trans Nanobioscience ; 14(8): 952-9, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26552090

RESUMO

This study focuses on the successful recrystallization of bacterial S-layer arrays of the Lactobacillus acidophilus ATCC 4356 at textile surfaces to create a novel method and material. Optimum bacterial growth was obtained at approximately 45 °C, pH 5.0, and 14 h pi. The cells were resuspended in guanidine hydrochloride and the 43 kDa S-protein was dialyzed and purified. The optimum reassembly on the polypropylene fabric surface in terms of scanning electron microscopy (SEM), reflectance, and uniformity (spectrophotometry) was obtained at 30 °C, pH 5.0 for 30 minutes in the presence of 2 gr/l (liquor ratio; 1:40) of the S-protein. Overall, our data showed that the functional aspects and specialty applications of the fabric would be very attractive for the textile and related sciences, and result in advanced technical textiles.


Assuntos
Materiais Biocompatíveis/química , Biotecnologia/métodos , Glicoproteínas de Membrana/química , Nanotecnologia/métodos , Têxteis , Cristalização , Interações Hidrofóbicas e Hidrofílicas , Lactobacillus acidophilus/química , Teste de Materiais
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