RESUMO
Fourteen Streptomyces strains reported earlier as plant growth promoters (PGP) in chickpea were characterized for production of ammonia and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and solubilization of silica and zinc. The results showed that nine (CAI-17, CAI-78, KAI-26, CAI-21, CAI-26, MMA-32, CAI-140, CAI-155 and KAI-180) and six (CAI-17, CAI-21, CAI-26, CAI-13, CAI-93 and KAI-180) strains were found to produce ammonia and ACC deaminase, respectively, while one (KAI-180) and eight (CAI-17, CAI-21, CAI-26, MMA-32, CAI-13, CAI-85, CAI-93 and KAI-180) strains solubilized silica and zinc, respectively. The selected 14 Streptomyces strains were categorized into three consortia groups, consortium-1 (CAI-17, CAI-68, CAI-78, KAI-26 and KAI-27), consortium-2 (CAI-21, CAI-26 and MMA-32) and consortium-3 (CAI-13, CAI-85, CAI-93, CAI-140, CAI-155 and KAI-180), based on their compatibility, and evaluated for their PGP traits in chickpea. The experiment was conducted under field conditions with two chickpea varieties over two years. The consortia-treated plots enhanced nodule number up to 23%, nodule weight up to 36%, root weight up to 27% and shoot weight up to 26% at 30 days after sowing and pod weight up to 35%, pod number up to 34% and grain yield up to 24% at harvest over the un-inoculated control plots. The harvested grains of consortia treatments were found to enhance crude protein up to 14%, crude fibre up to 17% and crude fat up to 16% over the grains from un-inoculated control. The rhizosphere soils of the consortia-treated plots enhanced total nitrogen up to 21%, organic carbon up to 8% and available phosphorous up to 16% over the un-inoculated control plots. This investigation demonstrated the potential use of the selected consortium of Streptomyces strains in the farmers' fields to improve the chickpea yields and soil fertility.
RESUMO
Chickpea is an inexpensive source of protein, minerals, and vitamins to the poor people living in arid and semi-arid regions of Southern Asia and Sub-Saharan Africa. New chickpea cultivars with enhanced levels of protein, Fe and Zn content are a medium-term strategy for supplying essential nutrients for human health and reducing malnutrition. In the current study, a chickpea reference set of 280 accessions, including landraces, breeding lines, and advanced cultivars, was evaluated for grain protein, Fe, Zn content and agronomic traits over two seasons. Using a mid-density 5k SNP array, 4603 highly informative SNPs distributed across the chickpea genome were used for GWAS analysis. Population structure analysis revealed three subpopulations (K = 3). Linkage disequilibrium (LD) was extensive, and LD decay was relatively low. A total of 20 and 46 marker-trait associations (MTAs) were identified for grain nutrient and agronomic traits, respectively, using FarmCPU and BLINK models. Of which seven SNPs for grain protein, twelve for Fe, and one for Zn content were distributed on chromosomes 1, 4, 6, and 7. The marker S4_4477846 on chr4 was found to be co-associated with grain protein over seasons. The markers S1_11613376 and S1_2772537 co-associated with grain Fe content under NSII and pooled seasons and S7_9379786 marker under NSI and pooled seasons. The markers S4_31996956 co-associated with grain Fe and days to maturity. SNP annotation of associated markers were found to be related to gene functions of metal ion binding, transporters, protein kinases, transcription factors, and many more functions involved in plant metabolism along with Fe and protein homeostasis. The identified significant MTAs has potential use in marker-assisted selection for developing nutrient-rich chickpea cultivars after validation in the breeding populations.
