Efficient Bioimaging with Diaminodicyanoquinodimethanes: Selective Imaging of Epidermal and Stomatal Cells and Insight into the Molecular Level Interactions.

The enhanced fluorescence emission of diaminodicyanoquinodimethanes (DADQs) in rigid and aggregated states holds great promise for bioimaging applications. This is demonstrated through their efficient application in epidermal and stomatal imaging with selective staining of cell walls and nuclei. Major advantages include the small quantities (a few nmols) of the fluorophore required, choice of DADQs soluble in water and organic solvents, and quick staining of the specimen in buffer-free state and in buffer medium. The molecular level interactions that enable staining are unraveled through isothermal calorimetry, infra-red spectroscopy and microscopy with energy dispersive X-ray spectroscopy analysis. It is proposed that DADQs with ionic or H-bonding functionalities bind to the polygalacturonic acid moieties in the epidermal layer; the former can bind also to nucleic acid polyanions. Fluorescence experiments explain the emission enhancement that enables the efficient imaging. DADQs are easy to synthesize, non-cytotoxic, and thermally, chemically and photo-stable, requiring no special storage conditions; preliminary experiments point to their potential utility in imaging different classes of cells.

Optical materials based on molecular nanoparticles.

A major part of contemporary nanomaterials research is focused on metal and semiconductor nanoparticles, constituted of extended lattices of atoms or ions. Molecular nanoparticles assembled from small molecules through non-covalent interactions are relatively less explored but equally fascinating materials. Their unique and versatile characteristics have attracted considerable attention in recent years, establishing their identity and status as a novel class of nanomaterials. Optical characteristics of molecular nanoparticles capture the essence of their nanoscale features and form the basis of a variety of applications. This review describes the advances made in the field of fabrication of molecular nanoparticles, the wide spectrum of their optical and nonlinear optical characteristics and explorations of the potential applications that exploit their unique optical attributes.

Polyelectrolyte-assisted formation of molecular nanoparticles exhibiting strongly enhanced fluorescence.

A polyelectrolyte-assisted reprecipitation method is developed to fabricate nanoparticles of highly soluble molecules. The approach is demonstrated by using a zwitterionic diaminodicyanoquinodimethane molecule bearing remote ammonium functionalities with high solubility in water as well as organic solvents. Nanoparticles are prepared by injecting aqueous solutions of this compound containing an optimum concentration of sodium poly(styrenesulfonate) into methanol. The strong fluorescence exhibited by the compound in the aggregated state is reflected in the enhanced fluorescence of the polyelectrolyte complex in water. The nanoparticles formed in the colloidal state manifest even stronger fluorescence, which leads to an overall enhancement by about 90 times relative to aqueous solutions of the pure compound. The conditions for achieving the emission enhancement are optimized and a model for the molecular-level interactions and aggregation effects is developed through a range of spectroscopy, microscopy, and calorimetry investigations and control experiments.