A comparative evaluation of the effect of 8% and 17% ethylenediaminetetraacetic acid exposure for 1 min and 10 min on the fracture resistance of endodontically treated roots: An in vitro study.

OBJECTIVE: The objective of this study was to evaluate the effect of 8% and 17% ethylenediaminetetraacetic acid (EDTA) exposure for 1 min and 10 min on the fracture resistance of endodontically treated roots. METHODOLOGY: Sixty human single-rooted teeth were decoronated and divided into six groups (n = 10). Canal preparation was carried out except in negative control group, using the ProTaper rotary file system. Final irrigation was performed using distilled water, 17% EDTA for 1 min and 10 min, 8% EDTA for 1 min and 10 min. Thereafter, roots were obturated with ProTaper F3 Gutta-percha points and AH Plus sealer using a single-cone technique. The specimens were loaded vertically at 1 mm/min crosshead speed until vertical root fracture occurred. Results were evaluated statistically with one-way analysis of variance and post hoc Tukey test. RESULTS: Analysis of results showed that the unprepared roots showed the highest fracture resistance and roots irrigated with 17% EDTA for 10 min showed the lowest fracture resistance. The mean fracture resistance of unprepared roots, roots irrigated with 8% EDTA for 10 min and 17% EDTA for 1 min was significantly higher than roots irrigated with 8% EDTA for 1 min, 17% EDTA for 10 min and distilled water. CONCLUSION: From a clinical viewpoint, if EDTA has to be used, it is safer to use higher concentration for a shorter application time or a low concentration with a longer application time. Prolonged use of high concentrations of EDTA might increase the risk of root fracture.

Antimicrobial effect of three disinfecting agents on Resilon cones and their effect on surface topography: An in vitro study.

AIM: The objective of this study was to evaluate the effectiveness of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 2% peracetic acid (PAA) in disinfecting Resilon cones and to evaluate topographical changes microscopically under scanning electron microscope (SEM) after rapid chemical disinfection. MATERIALS AND METHODS: Resilon cones were disinfected in an ultraviolet (UV) light chamber for 20 min and contaminated by immersing in a microbial suspension of Enterococcus faecalis for 30 min. The contaminated cones were then immersed in the 5.25% NaOCl, 2% CHX, and 2% PAA for 1 min, 5 min, and 10 min, separately. The cones were then incubated at 37°C in thioglycollate broth for 7 days and examined for turbidity. The samples showing turbidity were subcultured on blood agar and incubated at 37°C for 48 h. Gram staining was done to confirm that the cultured bacteria were E. faecalis. Surface changes of disinfected Resilon cones were evaluated under SEM. STATISTICAL ANALYSIS USED: The data were analyzed statistically using Kruskal-Wallis, analysis of variance (ANOVA), and Mann-Whitney U-test. RESULT: In eliminating E. faecalis, 5.25% NaOCl was most effective followed by 2% PAA and 2% CHX. Topographic examination of tested Resilon cones revealed some surface deposits after disinfection with 5.25% NaOCl and 2% CHX, whereas 2% PAA caused surface erosion. CONCLUSION: In disinfecting Resilon cones, 5.25% NaOCl is most effective followed by 2% PAA and 2% CHX.

Disinfection of gutta-percha cones using three reagents and their residual effects.

AIMS AND OBJECTIVES: The purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and MTAD in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. MATERIALS AND METHODS: Gutta-percha cones were immersed in suspensions of Enterococcus faecalis and Staphylococcus aureus separately. The cones were then immersed in 5.25% NaOCl, 2% CHX and MTAD for 30 seconds, 1 minute and 5 minutes separately. The disinfected cones were then incubated in thioglycollate media for 7 days. The thioglycolate media was sub-cultured and colony forming units were counted. For topographical examination of gutta-percha cones, the cones were immersed in respective solutions for one minute and allowed to air dry for 30 minutes after rinsing or without rinsing the cones with distilled water. The topography of the cones were then analyzed under SEM. Data was statistically analyzed using one way anova. RESULTS: MTAD was found to be the most effective disinfecting solution. The topographical examination of GP cones found some deposits after the disinfection procedure in every group. These deposits were removed when the GP cones were rinsed with distilled water. CONCLUSIONS: MTAD possesses superior bactericidal activity when compared with NaOCl and CHX and a final rinse with distilled water is essential after the disinfection procedure.

An in vitro evaluation of effect of eugenol exposure time on the shear bond strength of two-step and one-step self-etching adhesives to dentin.

OBJECTIVES: To evaluate the effect of the eugenol exposure time of an eugenol-based provisional restorative material on the shear bond strength of two-step and one-step self-etching adhesives to dentin, at three different time intervals of 24 h, 7 days, and 14 days. MATERIALS AND METHODS: Forty extracted human posterior teeth were sectioned mesiodistally to obtain two halves and the resulting 80 halves were randomly assigned into four groups of 20 specimens each (Group-I, -II, -III, and -IV). Cavities of specified dimensions were prepared to expose dentin surface. In Group-I, temporarization was carried out with noneugenol cement (Orafil-G) for 24 h (control group). In Group-II, -III, and -IV, temporarization was carried out with eugenol cement (intermediate restorative material (IRM)) for 24 h, 7 days, and 14 days, respectively. Each group was further divided into two subgroups of 10 teeth each for two-step (Adper SE Plus) and one-step (Adper Easy One) self-etch adhesive systems, respectively. A plastic tube loaded with microhybrid composite resin (Filtek Z-350, 3M) was placed over the dentin surface and light cured. The specimens were subjected to shear stress in universal testing machine. RESULTS: Group-II yielded low shear bond strength values compared with Group-III, -IV, and Group-I, which was statistically significant. CONCLUSIONS: The prior use of eugenol containing temporary restorative material reduced the bond strength of self-etch adhesive systems at 24-h period. No reduction in bond strength at 7 or 14 days exposure was observed with either two-step or one-step self-etch adhesive.