RESUMO
Citric acid is an organic acid extensively used in feed industry, and AZOMITE is a hydrated aluminosilicate compound rich in rare earth elements and trace mineral elements. This study investigated the supplemental effects of AZOMITE and citric acid individual or in combination on the growth performance, intestinal microbiota, morphology, digestive enzyme activity, serum indexes, and disease resistance of juvenile largemouth bass. Six diets were designed, including the control diet (CON) and the five additive-supplemented diets with the addition of 4 or 8 g/kg citric acid (CA4, CA8), 3 g/kg AZOMITE (A3), and their combined addition as 4 g/kg citric acid + 1.5 g/kg AZOMITE) (C4A1.5) and 8 g/kg citric acid + 3 g/kg AZOMITE (C8A3). Juvenile largemouth bass with initial body weight of 22.01 ± 0.09 g were fed the six diets for 56 days. The results revealed that the combined addition of 4 g/kg citric acid and 1.5 g/kg AZOMITE (C4A1.5) increased weight gain by 7.99% (P < 0.05), and decreased feed conversion ratio by 0.07 (P < 0.05). The protein retention in the C4A1.5 group and the lipid retention in all additive-supplemented groups were significantly higher than those in the control group (P < 0.05). In serum, all additive-supplemented groups showed significantly higher glutathione peroxidase activity than the control group (P < 0.05). The activities of superoxide dismutase and catalase in the CA8, A3, C4A1.5, and C8A3 groups were significantly higher (P < 0.05), while the concentration of malondialdehyde was significantly lower than those in the control group (P < 0.05). Moreover, the total antioxidant capacity in the A3 and C4A1.5 groups, and lysozyme activity in the A3, C4A1.5, and C8A3 groups were significantly increased when compared to the control group (P < 0.05). In digestive enzyme, the protease activity in the A3, C4A1.5 groups, and amylase activity in the CA4, CA8, and C4A1.5 groups were significantly higher than those in the control group (P < 0.05). In intestinal microbiota, Firmicutes abundance was elevated in all additive groups, while the Fusobacteriota and Plesiomonas shigelloides abundance were decreased. In the intestinal histology, the CA8, A3, and C4A1.5 groups showed significantly higher villus height than the control group (P < 0.05). After the infection with Aeromonas hydrophila, the cumulative mortality of all additive-supplemented groups was significantly lower (P < 0.05), and the C4A1.5 group demonstrated the lowest mortality. In conclusion, the combined supplementation of 4 g/kg citric acid + 1.5 g/kg AZOMITE increased the growth, antioxidant, immune capacity, improved the intestinal morphology and microbial flora of juvenile largemouth bass, and promoted the resistance against Aeromonas hydrophila infection.
RESUMO
OBJECTIVE: To describe the imaging findings of polyethylene liner dissociation in total hip arthroplasty. MATERIALS AND METHODS: Retrospective search of our institution's radiology database identified 12 patients with polyethylene liner dissociation of a total hip arthroplasty. Clinical and operative notes were reviewed. All radiological studies were reviewed independently by two radiologists. RESULTS: Among 12 patients (seven females/five males; mean age: 67 years; median interval after surgery at diagnosis: 8.5 months) with polyethylene liner dissociation, 11 had radiographs, six had CT, seven had MRI, and two had arthrography. "Bubble sign" and "crescent sign" on radiography were insensitive, seen only in three patients, but all showed abrupt eccentric positioning of the femoral head. CT identified the dislocated liner in five of six patients but failed to identify one liner, which was partially associated with the acetabular cup. MRI identified the dislocated liner in all seven patients, with the MAVRIC (multiacquisition variable resonance image combination) sequences either increasing the diagnostic confidence relative to the routine pulse sequences or being the sole sequences in which the liner can be identified if the liner remained partially associated with the acetabular cup. Arthrography identified the dislocated liner in one of two patients. CONCLUSIONS: Previously described radiographic signs of polyethylene liner dissociation are insensitive, but abrupt eccentric positioning of the femoral head in the correct clinical context is highly suggestive of the diagnosis. MRI and CT can accurately localize the displaced liner. MAVRIC is particularly helpful if the dislocated liner remains partially associated with the acetabular cup.
