Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Kaohsiung J Med Sci ; 38(10): 933-949, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36054559

RESUMO

This study aimed to identify the molecular mechanism underlying NEAT1 regulation of non-small-cell lung cancer (NSCLC) autophagy and apoptosis. The expression levels of NEAT1, miR-128-3p, and ADAM28 in NSCLC tissues and cells were examined using qRT-PCR. The relationships between NEAT1, miR-128-3p, and ADAM28 expression levels and prognosis of NSCLC patients were investigated using the Kaplan-Meier analysis method. The interactions between NEAT1, miR-128-3p, and ADAM28 were confirmed by dual-luciferase reporter assay or FISH assay. Cell proliferation and apoptosis were detected by CCK-8 and flow cytometry assays, respectively. Apoptosis and autophagy-related proteins Bax, cleaved caspase-3, cleaved caspase-9, Bcl-2, LC3, Beclin-1, and p62 were analyzed by western blotting. Finally, an in vivo NSCLC mouse xenograft model was established to confirm the in vitro data. We showed NEAT1 and ADAM28 expression levels were upregulated, while the miR-128-3p level was downregulated in NSCLC tissues and cells. NSCLC patients with high NEAT1 expression levels, low miR-128-3p levels, or high ADAM28 levels had significantly reduced overall survival times. Silencing of NEAT1 inhibited NSCLC cell autophagy and promoted apoptosis by sponging miR-128-3p. MiR-128-3p directly targeted ADAM28 and suppressed ADAM28 expression, which led to deactivation of the JAK2/STAT3 signaling pathway. Furthermore, ADAM28 overexpression attenuated miR-128-3p overexpression-induced apoptosis and autophagy inhibition in NSCLC by increasing the phosphorylation of JAK2 and STAT3. NEAT1 depletion or miR-128-3p overexpression inhibited autophagy and promoted apoptosis in vivo by suppressing ADAM28. In other word, silencing NEAT1 inhibited autophagy and then promoted NSCLC cell apoptosis by deactivating the JAK2/STAT3 signaling pathway through regulation of miR-128-3p/ADAM28 axis.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , MicroRNAs , RNA Longo não Codificante , Proteínas ADAM , Animais , Apoptose/genética , Autofagia/genética , Proteína Beclina-1/genética , Proteína Beclina-1/metabolismo , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Caspase 3/metabolismo , Caspase 9/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/genética , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Camundongos , MicroRNAs/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , RNA Longo não Codificante/metabolismo , Sincalida/metabolismo , Proteína X Associada a bcl-2/metabolismo
2.
Medicine (Baltimore) ; 97(23): e10774, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29879014

RESUMO

RATIONALE: Monomelic amyotrophy (MMA) is a benign motor neuron disease with bilateral muscular atrophy in asymmetry and abnormal in the electromyography (EMG). However, we report a case by the muscle biopsy which shows symptoms of slowly progressive amyotrophy despite having a normal EMG. PATIENT CONCERNS: A 51-year-old male was diagnosed with a lower limb amyotrophy, insidious at the onset and located in the distal thigh and the proximal crus near the knee, slowly progressive weakness, and wasting of his right gastrocnemius muscle for the last 20 years. DIAGNOSES: He was diagnosed with MMA by the clinical profile, natural history, examinations, and the biopsy. INTERVENTIONS: We perform dynamic physical therapy for the patient in this case. OUTCOMES: The positive effects of dynamic physical therapy in this case with MMA were shown in this report. LESSONS: The outcome of physical therapy is satisfactory.


Assuntos
Terapia por Exercício/métodos , Extremidade Inferior/patologia , Músculo Esquelético/patologia , Atrofias Musculares Espinais da Infância/diagnóstico , Adulto , Biópsia , Eletromiografia , Humanos , Masculino , Pessoa de Meia-Idade , Atrofias Musculares Espinais da Infância/reabilitação
3.
J Thorac Dis ; 8(8): 2259-63, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27621887

RESUMO

Uniportal video-assisted thoracic surgery (VATS) anatomical pulmonary resection, with only one small incision for surgery instruments and camera insertion, requires higher operative skills, especially in the cases of the enlarged pulmonary hilar lymph nodes. With improved technology and increased experiences in VATS lobectomy, uniportal VATS lobectomy has been applied in major medical centers recently. A 67-year-old male patient with left upper peripheral lung cancer and enlarged hilar lymph nodes underwent unipotal VATS lobectomy and systemic mediastinal lymph node dissection. The patient recovered uneventfully.

4.
J Thorac Dis ; 6(12): 1826-30, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25589982

RESUMO

Sleeve lobectomy for selected cases of central lung cancer has better functional outcomes comparing to pneumonectomy. With improved technology and increased experiences in complete video-assisted thoracic surgery (VATS) lobectomy, complete VATS sleeve lobectomy has been applied in major medical centers recently. A 64-year-old male patient with left lower central lung cancer underwent thoracoscopic sleeve lobectomy and systemic mediastinal lymph node dissection. The major incision, of four incisions in total, was a 4 cm mini-incision in the 4th intercostal space of anterior axillary line. The patient had recovered uneventfully after the surgery.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...