Proposals on the control of VERO cells used to prepare inactivated poliomyelitis vaccine.

The purpose of this study is to know if it is possible to prepare inactivated poliomyelitis vaccines on Vero cell culture. In the cloned cell line used to prepare the vaccines, we have looked for an eventual toxic effect by different system tests. The cells between the 148e and 151e passage used to prepare the vaccines were inoculated into: - suckling mice, rabbits and guinea pigs - embryonated eggs. The supernatant fluid of the cells was inoculated into: - Vero cells - Primary rabbit kidney cells - Primary monkey kidney cells. We have also looked for an eventual tumorigenic effect on immunodepressed mice with an antithymocytic serum. The results of the assays are presented and discussed.

[Titration of serum and antirabies immunoglobulins by the radial immunodiffusion method]. / Le titrage du serum et des immunoglobulines antirabioues par la méthode d'immunodiffusion radiale.

Titration on mice of sera and immunoglobulin rabies anibodies is expensive, tedious and long. We are proposing a cheap, easy and rapid method which could be useful during the preparation of immunoglobulin or for the final titration. The antigen is a purified inactivated vaccine cultured on human diploid cells and purified by dextran sulfate and ultracentrifugation; it is included in agar on a glass slide. The reference serum was given us by Dr Atanasiu from Pasteur Institute; its titre was compared with international reference on mice test and was given a titre of 65 IU per ml. Five specific rabies immunoglobulin and 3 sera were received from two producers for titration. For IDR test, immunoglobulin and reference serum dilutions are diposed in wells of the same agar slide and then incubated at ambiant room temperature during 48 hours. Migration of each Ig and serum dilution is measured after coloration reported on a graph and compared. Each of the samples was checked 4 times by radial immunodiffusion test and twice by seroneutralization test on mice. Statistical analysis of the results by Student's test demonstrates that both methods are comparable.