Manosonication extraction of RG-I pectic polysaccharides from citrus waste: Optimization and kinetics analysis.

To better understanding the potential of manosonication to accelerate the extraction of RG-I pectic polysaccharides from citrus wastes, alkaline-mediated manosonication extraction (MSE) was optimized using a Box-Behnken design, and the extraction kinetics model was analyzed. The single-factor method revealed that NaOH significantly impacted on the yield and RG-I characterizations (Rha mol% and (Gal+Ara)/Rha ratio), whereas other factors were focused on influences of yields. In the developed quadratic polynomial model, the maximum extraction yield of 25.51 ±â€¯0.81 % was obtained with sonication at 42 ℃, 40 % amplitude, and 250 kPa for 20 min. The kinetics study demonstrated that MSE facilitated the extractability, dissolution and degradation of pectin, resulting in the highest extractability of 27.83 % compared with ultrasonic extraction (22.86 %) and alkaline extraction at high (24.71 %) and low temperature (20.21 %). Rheology and thermal analyses verified the change in polymerization by MSE and the potential functional applications of the RG-I pectic polysaccharides.

Manothermosonication (MTS) treatment by a continuous-flow system: Effects on the degradation kinetics and microstructural characteristics of citrus pectin.

Modified pectin (MP) was reported to have increased bioactivities compared with the original one. However, traditional modification methods such as using an acidic solvent with heating are not only costly but causing severe pollution as well. In this study, manothermosonication (MTS) with a continuous-flow system was utilized to modify citrus pectin. The citrus pectin (5 g/L) treated by MTS (3.23 W/mL, 400 kPa, 45 °C) exhibited lower molecular weight (Mw, 248.17 kDa) and PDI (2.76). The pectin treated by MTS (400 KPa, 45 °C, 5 min) exhibited a narrower Mw distribution and lowered more Mw (48.8%) than the ultrasound(US)-treated (23.8%). Pectin degradation data fitted well to kinetic model of 1/Mwt -1/Mw0 = kt (45-65 °C). A lower activation energy of 13.33 kJ/mol was observed in the MTS treatment compared with the US-treated (16.38 kJ/mol). The MTS-treated pectin lowered the degree of methoxylation (DM), mol% of rhamnose and galacturonic acid (GalA) while increased mol% of galactose (Gal), xylose (Xyl), and arabinose (Ara). The 1H and 13C nuclear magnetic resonance showed that MTS could not alter the primary structures of citrus pectin. However, an elevated (Gal + Ara)/Rha and reduced GalA/(Rha + Ara + Gal + Xyl) molar ratios after MTS suggested that MTS resulted in more significant degradation on the main chains and less on the side chains of pectin, in agreement with the result of atomic force microscope. Moreover, the MTS-treated pectin exhibited a higher 1,1-diphenyl-2picryl hydrazyl radical scavenging capacity compared with original pectin.

Effect of ultrasonication and thermal and pressure treatments, individually and combined, on inactivation of Bacillus cereus spores.

Bacillus cereus spores are a concern to the food industry due to their high resistance to processing and their ability to germinate to vegetative cells under suitable conditions. This research aimed to elucidate the mechanisms of Bacillus cereus spore inactivation under ultrasonication (US) combined with thermal (thermosonication, TS) treatments, with pressure (manosonication, MS) treatments, and with thermal and pressure (manothermosonication, MTS) treatments. Electronic microscopy, dipicolinic acid (DPA) release, and flow cytometric assessments were used to investigate the inactivation effect and understand the inactivation mechanisms. The sporicidal effects of the US and thermal treatment were slight, and the MS and TS also showed little inactivation effect. However, ultrasonication promoted the detachment of the exosporium, thereby reducing the spore's ability to adhere to a surface, while the thermal treatment induced a decrease in the electron density in the nucleoid of bacterium, which retained a relatively intact exosporium and coat. MS caused 92.54% DPA release, which might be due to triggering of the germinant receptors or releasing of ions and Ca2+-DPA. In addition, the morphological changes such as core hydration and cortex degradation were significant after treatment with MS. The release of DPA and the morphological changes were responsible for the reduction in thermal resistance. The MTS showed a remarkable inactivation effect of 3.12 log CFU/mL reductions after 30 min of treatment. It was the most effective treatment and exhibited a large fraction of damage. In addition, the MTS had a significant impact on the intracellular structure of the spores, with the coat destroyed and the cortex damaged. These results indicated that ultrasonication combined with thermal and pressure treatments had a significant sporicidal effect on Bacillus cereus spores and could be a promising green sterilization technology.

Acoustic cavitation assisted extraction of pectin from waste grapefruit peels: A green two-stage approach and its general mechanism.

In the present study, acoustic cavitation assisted extraction (ACAE) which is a green approach was used for two-stage extraction of pectin from waste grapefruit peels regarding the specific two stages and different peel particle sizes. The yields, physicochemical properties and structure prediction of pectin from different peel particle sizes and different stages by two methods were compared. Highest pectin yield of 23.49% was achieved at the peel size of 0.9mm and was slightly higher than the yield of 23.44% at finer particle size. ACAE achieved four times higher yield compared with conventional heating method (CHE) at the second stage. ACAE pectin which had lower molecular weight and degree of methoxylation was richer in rhamnogalacturonan-I (RG-I) region with long side chains compared with CHE pectin from chemical and FT-IR analysis. Based on the results, a potential general mechanism for ACAE of plant cell wall viscous polysaccharides was discussed in terms of the effects of cavitation on the swelling index and morphological study of residue to reveal the "barrier effect" during the process. Furthermore, the energy consumption for ACAE was significantly lower than the conventional method, indicating its promising application in industrial scale.

Ultrasound assisted enzymatic hydrolysis of starch catalyzed by glucoamylase: Investigation on starch properties and degradation kinetics.

The present work investigates the synergistic impact of glucoamylase and ultrasound on starch hydrolysis. The extent of starch hydrolysis at different reaction parameters (ultrasonic intensity, temperature, reaction time) was analyzed. The hydrolysis extent increased with the reaction time and reached a maximum value under ultrasonic intensity of 7.20W/mL at 10min. Ultrasound did not alter the optimum enzymatic temperature but speeded up the thermal inactivation of glucoamylase. The evaluation of enzymatic kinetics and starch degradation kinetics indicated a promotion of the reaction rate and enzyme-substrate affinity. According to the thermodynamic results, sonoenzymolysis reactions require less energy than enzymolysis reactions. The measurement of molecular weight, solubility, thermal properties, and structures of the substrates revealed that sonoenzymolysis reaction generated greater impacts on starch properties. The molecular weight and radii of gyration decreased by 80.19% and 90.05% respectively while the starch solubility improved by 136.50%.