RESUMO
Objective To explore the healthcare demands and utilization of soldiers in navy warship troops during maritime missions, so as to provide scientific basis for the relevant health agencies to make plan and allocate health resources. Methods Questionnaire were designed for naval soldiers to fill in, and the investigation included basic information of soldiers, disease occurrence, physical injury and medical treatment during the missions. The EpiData 3.1 was used for data input by double person and the SPSS 21.0 was used for statistical analysis. Results A total of 850 questionnaires were sent out and 755 valid questionnaires were returned (response rate 88.82%). During the maritime mission, the incidence of diseases of soldiers in navy warship troops was high, with various types of diseases. The diseases of high frequency were acute gastroenteritis (49.1%, 371/755) and heat stroke (36.4%, 275/755). The physical injuries of high incidence were seasickness (65.0%, 491/755), noise induced hearing loss (20.0%, 151/755), and cabin noxious gas poisoning (19.3%, 146/755). There were 242 persons (32.1%) who believed that the disease could not be solved during maritime missions. Conclusion During the maritime missions, the incidences of diseases and physical injuries of soldiers are high in navy warship troops, and the health services cannot well meet the demands of the soldiers.
RESUMO
rpoX, a Vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by SYBR Green I-based real-time PCR. The specificity of the detection was confirmed in different samples. The minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.
Assuntos
Proteínas de Bactérias/genética , Doenças dos Peixes/microbiologia , Compostos Orgânicos/química , Reação em Cadeia da Polimerase/métodos , Vibrio alginolyticus/genética , Animais , Benzotiazóis , Primers do DNA/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Diaminas , Doenças dos Peixes/diagnóstico , Perciformes , Quinolinas , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Fator sigma/genéticaRESUMO
A previous study has shown that Vibrio alginolyticus ZJ-51 undergoes colony phase variation between opaque/rugose (Op) and translucent/smooth (Tr). The AI-2 quorum-sensing master regulator ValR, a homolog to V. harveyi LuxR, was suggested to be involved in the transition. To investigate the role of ValR in the variation and in biofilm formation, an in-frame deletion of valR in both Op and Tr backgrounds was carried out. The mutants in both backgrounds showed an intermediate colony morphotype, where the colonies were less opaque/rugose but not fully translucent/smooth either. They also showed an intermediate level of motility. However, biofilm formation was severely decreased in both mutants and polar flagella were depleted also. Quantitative PCR showed that most of the genes related to flagellar and polysaccharide biosynthesis were upregulated in the mutant of Op background (Delta valR/Op) but downregulated in the mutant of Tr background (Delta valR/Tr) compared with their parental wild-type strains. This suggests that ValR may control biofilm formation by regulating flagellar biosynthesis and affect the expression of the genes involved in colony phase variation in V. alginolyticus.
