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Objective:To investigate the traditional Chinese medical (TCM) constitution status of young and middle-aged residents and to analyze related influencing factors.Methods:A cross-sectional study was conducted to obtain data of 1 082 young and middle-aged residents in China from April to May 2019. The general characteristic, habits, emotional status and TCM constitution types of young and middle-aged residents were been collected.Results:The number of normal constitution types was 415(38.35%, 415/1 082), and the number of biased constitution types was 667(61.65%, 667/1 082) which was dominated by qi-deficiency constitution (16.70%, 344/2 060), yang-deficiency constitution (16.17%, 333/2 060) and damp-heat constitution (15.97%, 329/2 060). The Logistic regression analysis showed that the dietary regularity, taking vigorous exercise and taking moderate exercise were the protective factors of the biased constitution types of young and middle-aged residents( OR values were 0.624, 0.704, 0.704, all P<0.05), while the female, meat-based diet, anxiety and depression were the risk factors of the biased constitution types of young and middle-aged residents( OR values were 2.009-2.568, all P<0.01). Conclusions:The biased constitution is common among young and middle-aged residents. The risk factors of biased constitution are unhealthy lifestyle, anxiety and depression,which suggest the community to develop actively interventions to adjust the lifestyle and strengthen the maintenance of mental health of young and middle-aged residents.
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Objective: To investigate the inhibitory effect of epigallocatechin-3-gallate (EGCG) on human osteoscarcoma cell line 143B, and its possible molecular mechanism. Methods: Human osteoscarcoma 143B cells were treated with 0-50 μmol/L EGCG. Then the proliferation, apoptosis and migration of143B cells were measured by crystal violet staining, hoechst staining, cell wound-healing and Transwell chamber assays, respectively. The expression levels of apoptosis-and migrationrelated proteins were detected by Western blotting. The activity of Wnt/β-catenin signaling pathway was detected by luciferase reporter assay. Furthermore, the protein expression levels of β-catenin and its downstream target molecules c-Myc and cyclin D1 were detected by Western blotting. Results: After treatment with EGCG at highly concentration, the proliferation and migration of 143B cells were significantly inhibited (both P < 0.05), but the proportion of apoptotic cells was markedly increased (P < 0.05). Compared with the EGCG-untreated group, EGCG up-regulated the expressions of caspase-3 and its cleaved protein (both P < 0.05), and down-regulated the expressions of Bcl-2, matrix metalloproteinase-2 (MMP-2), β-catenin, c-Myc and cyclin D1 proteins (all P < 0.05). In addition, the luciferase activity reflecting Wnt/ β-catenin signaling pathway activity in EGCG-Treated group was significantly lower than that in EGCG-untreated group (P < 0.05). Conclusion: EGCG can inhibit the proliferation and migration of osteoscarcoma 143B cells, and promote apoptosis, which may be related to the blocking of Wnt/β-catenin signaling pathway.
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Objective To investigate and analyze the status quo of professional identity of nursing students with different educational backgrounds.Methods With stratified-cluster random sampling,635 students were surveyed using the nursing students' professional identity questionnaire.Results The total score of professional identity of junior college nursing students was significantly higher than that of undergraduate students.Junior college nursing students had a higher score in professional knowledge dimension than undergraduate and graduate students,while the score of nursing undergraduate students was higher than that of graduate students.Junior college nursing students had a higher score in professional will dimension than undergraduate and graduate students.Nursing graduate students had a higher score in professional expectation dimension than junior college and undergraduate students,while the score of undergraduate students was higher than that of junior college students.Whatever the educational background,professional skill and professional expectation were the top two dimensions,while professional knowledge and professional emotion were the last two dimensions.The difference was statistically significant between the answers of nursing students with different educational backgrounds to the following questions,such as which is the most probable factor affecting your learning enthusiasm,who has the greatest impact on your professional thought about nursing and which nursing field are you most willing to go in for.There was significant difference in professional identity score between different grades of junior college and undergraduate nursing students,while there is no significant grade difference in graduate students.Conclusions There were some differences of professional identity of nursing students with different educational backgrounds.Nursing educators should carry out the professional education according to the student's educational backgrounds.
