Plasma prolactin, thyroid-stimulating hormone, melanocyte-stimulating hormone, and adrenocorticotropin responses to thyrotropin-releasing hormone in mares treated with detomidine and butorphanol.

Stress or excitement is a concern when performing endocrine tests on fractious horses. Sedation may be a solution; however, perturbation of test results may preclude useful information. Thyrotropin-releasing hormone (TRH) is a known stimulator of prolactin, thyroid-stimulating hormone (TSH), melanocyte-stimulating hormone (MSH), and ACTH. Thyrotropin-releasing hormone-induced ACTH is a diagnostic tool for the assessment of endocrinopathies such as pituitary pars intermedia dysfunction. It is unknown if drugs commonly used for sedation alter endocrine responses. The objective of this study was to assess the effects of detomidine (DET) and butorphanol on endocrine responses to TRH. Nine light horse mares were used in a replicated 3 × 3 Latin square with the following treatments: saline, DET, and detomidine + butorphanol (DET/BUT), all administered intravenously at 0.01 mg/kg BW. A 1-wk washout period was allowed between phases, all of which were performed in December. Blood samples were collected at -10 and 0 min before treatment and 5 and 10 min post-treatment. Administration of 1 mg TRH occurred 10 min post-treatment, and blood sampling continued 5, 10, 20, and 30 min post-TRH. Data were analyzed by ANOVA as a replicated Latin square with repeated sampling. Plasma prolactin increased (P < 0.0001) after TRH in all groups, rapidly peaking at 5 min in drug-treated mares and 40 min in saline-treated mares. The peak prolactin response to TRH was 2-fold higher (P < 0.0001) in saline-treated mares compared with those drug-treated. A peak rise in plasma TSH was observed in DET/BUT-treated mares 10 min after TSH and was greater (P ≤ 0.007) compared with DET- and saline-treated mares. Plasma MSH was stimulated (P = 0.001) by DET and DET/BUT before TRH, and the peak MSH response to TRH was greater (P < 0.0001) in drug-treated mares, although not hastened as observed with prolactin and TSH. A peak rise in ACTH was observed in drug-treated mares 5 min after administration of TRH, whereas a peak rise was observed in control mares 10 min post-TRH and was almost 2-fold lower (P = 0.05) than the peak observed in DET and DET/BUT-treated mares. Basal ACTH concentrations were not affected by DET or DET/BUT, indicating that sedation with these compounds may be achieved when needing to measure basal plasma ACTH. Treatment with DET and DET/BUT did alter the prolactin, TSH, MSH, and ACTH responses to TRH; therefore, the use of these drugs may not be advisable when assessing endocrine responses to TRH stimulation.

Are the UK systems of innovation and evaluation of medical devices compatible? The role of NICE's Medical Technologies Evaluation Programme (MTEP).

The economic evaluation of medical products and services is increasingly prioritised by healthcare decision makers and plays a key role in informing funding allocation decisions. It is well known that there are a number of methodological difficulties in the health technology assessment of medical devices, particularly in the provision of efficacy evidence. By contrasting devices with pharmaceuticals, the way in which the differing systems of innovation mould the UK's industry landscape is described and substantiated with market statistics. In recognition of the challenges faced by industry, as well as the growing need for cost-effective allocation of National Health Service (NHS) resources, the National Institute for Health and Care Excellence (NICE) led the development of the Medical Technologies Evaluation Programme (MTEP), which launched in 2009/2010. The review of the UK's medical devices market supports the programme's three principal aims: to simplify access to evaluation, speed up the process, and increase evaluative capacity for devices within NICE. However, an analysis of the output of MTEP's first 3 years suggests that it has some way to go to meet each of these aims.

Methane production potential (B0) of swine and cattle manures--a Canadian perspective.

Canada's agricultural emissions accounted for 60 Mt or 8% of national greenhouse gas (GHG) emissions in 2007. The estimation of CH4 emission factor (B0) from manure management systems in Canada is prone to uncertainty owing to lack of B0 values for Canadian conditions. Therefore, in this study, manure samples from six Canadian animal farms, two each of swine, beef and dairy cattle, were investigated in order to estimate their methane production potential (B0). The ultimate anaerobic biodegradability was measured with ISO standard batch fermentation. The extent of biodegradation of the manure samples with or without sodium benzoate was always greater than 60% and hence showed no inhibitory effect on methane production by the manure. The impact of use of antibiotics in the animal feed on methane production was also considered; however, no inhibitory effect on methane production could be observed. The plateau of methane production in all cases was achieved by 63 d of anaerobic digestion process and the final pH was within 6-8. The calculated B0 were in the range of 0.47-0.42, 0.21-0.19 and 0.35-0.30 for swine, beef cattle and dairy cattle, respectively. The uncertainties associated with B0 values were +/- 9% for swine, +/- 3% for beef cattle and, +/- 6 and +/- 2% for dairy cows.

P-selectin antagonism causes dose-dependent venous thrombosis inhibition.

Inhibition of P-selectin by antibody or selectin antagonist decreases inflammation and thrombosis. This study evaluates the dose-response relationship using a selectin receptor antagonist. Eight male baboons (Papio anubis) underwent inferior vena caval thrombosis using a 6 h balloon occlusion model. Three animals received 500 microg/kg P-selectin antagonist (rPSGL-Ig) and five 1 mg/kg rPSGL-Ig with or without a non-anticoagulant dose of Dalteparin. These animals were compared to our published results in this model with 4 saline controls and 8 animals that received 4 mg/kg rPSGL-Ig. A statistically significant dose-response relationship existed between rPSGL-Ig dose and thrombosis (p < 0.01), and between rPSGL-Ig dose and spontaneous recanalization (p<0.05). Inflammatory assessment revealed decreased gadolinium enhancement in all rPSGL-Ig groups compared to previously reported control, despite no significant differences in inflammatory cell extravasation. No dose of rPSGL-Ig caused anticoagulation. Selectin antagonism results in a dose-dependent decrease in thrombosis and increase in spontaneous recanalization.

Non-uniform distribution of cellular phenotypes in the optic tectum of the leopard frog.

Regional specialization in the retina have been described in a number of species. We have investigated whether such specializations can be found in the optic tectum, an area of the brain responsible for the processing of visual information. Using the tectum of Rana pipiens, we have examined the distribution of three different cell types defined on the basis of their immunoreactivity to somatostatin, substance P, and serotonin antibodies. These three immunoreactive cell populations had differing, nonuniform distributions in the optic tectum. Somatostatin-line immunoreactive cells were largely restricted to the caudal one-third of the tectum, whereas both substance P-like immunoreactive (SP-ir) and serotonin-like immunoreactive (5-HT-ir) cells were found unequally represented throughout the tectum. The percentage of SP-ir cells decreased significantly in both the posterior and medial directions from its high in the anterior lateral tectum. Although serotonin-like immunoreactivity was also greatest in the lateral tectum and decreased significantly medially, it was largely constant in the anterior-to-posterior dimension. The populations of SP-ir and 5-HT-ir cells were nonoverlapping. Our results suggest that information may be processed differently in different regions of the optic tectum.

Neuropeptide Y immunoreactivity of a projection from the lateral thalamic nucleus to the optic tectum of the leopard frog.

Using rhodamine-labelled latex beads as a retrograde tracer, we have shown that a subset of the neurons projecting from the lateral thalamic nucleus to the optic tectum of the leopard frog are neuropeptide Y-like immunoreactive (NPY-IR). In juvenile frogs, approximately twice as many lateral thalamic nucleus cells from this area project to the ipsilateral tectum as project to the contralateral tectum. NPY-IR cells make up 25% of the projection to the ipsilateral tectum and 13% of the projection to the contralateral tectum. The ipsilateral NPY-IR projection from the lateral nucleus was present in tadpoles and was similar in its characteristics to that found in the juvenile frog. However, the contralateral tectal projection was virtually nonexistent in these animals. The results of these experiments suggest that NPY from the lateral nucleus is released into the ipsilateral tectal neuropil in both the developing and adult frog.

Length and sequence variation in evening bat D-loop mtDNA.

Length variation in D-loop mitochondrial DNA was observed after amplification with the polymerase chain reaction (PCR) in 28% of 195 evening bats, Nycticeius humeralis, from seven colonies. Nucleotide sequences of PCR products show that this heteroplasmy is characterized by an 81-bp region which is tandemly repeated five to eight times. Southern blots using PCR products as probes on HaeIII genomic digests confirm the presence of heteroplasmy. Furthermore, densitometry of electrophoresed PCR products from 109 mother-offspring pairs indicate that heteroplasmy is stably transmitted from mother to offspring with one exception: a heteroplasmic offspring had a homoplastic mother and sib. Nucleotide sequences from this family reveal that a repeat duplication and deletion occurred. The observed mutation rate per generation, mu, for length polymorphism is comparable to an independent estimate, mu = 10(-2), based on hierarchical diversity statistics. With the exception of the repeat nearest the proline tRNA gene, sequence similarities between repeats within bats are consistent with a model of concerted evolution due to unidirectional replication slippage. Selection is inferred to act on the first repeat because in comparison to other repeats it has the least sequence divergence among bats, the fewest transversions, and the lowest minimum free energy associated with folding.