RESUMO
OBJECTIVE: To report on the effectiveness of a standardised core Maternity Waiting Home (MWH) model to increase facility deliveries among women living >10 km from a health facility. DESIGN: Quasi-experimental design with partial randomisation at the cluster level. SETTING: Seven rural districts in Zambia. POPULATION: Women delivering at 40 health facilities between June 2016 and August 2018. METHODS: Twenty intervention and 20 comparison sites were used to test whether MWHs increased facility delivery for women living in rural Zambia. Difference-in-differences (DID) methodology was used to examine the effectiveness of the core MWH model on our identified outcomes. MAIN OUTCOME MEASURES: Differences in the change from baseline to study period in the percentage of women living >10 km from a health facility who: (1) delivered at the health facility, (2) attended a postnatal care (PNC) visit and (3) were referred to a higher-level health facility between intervention and comparison group. RESULTS: We detected a significant difference in the percentage of deliveries at intervention facilities with the core MWH model for all women living >10 km away (DID 4.2%, 95% CI 0.6-7.6, P = 0.03), adolescent women (<18 years) living >10 km away (DID 18.1%, 95% CI 6.3-29.8, P = 0.002) and primigravida women living >10 km away (DID 9.3%, 95% CI 2.4-16.4, P = 0.01) and for women attending the first PNC visit (DID 17.8%, 95% CI 7.7-28, P < 0.001). CONCLUSION: The core MWH model was successful in increasing rates of facility delivery for women living >10 km from a healthcare facility, including adolescent women and primigravidas and attendance at the first PNC visit. TWEETABLE ABSTRACT: A core MWH model increased facility delivery for women living >10 km from a health facility including adolescents and primigravidas in Zambia.
Assuntos
Parto Obstétrico/estatística & dados numéricos , Serviços de Saúde Materna/estatística & dados numéricos , Assistência Centrada no Paciente/estatística & dados numéricos , Serviços de Saúde Rural/estatística & dados numéricos , População Rural/estatística & dados numéricos , Adolescente , Adulto , Análise por Conglomerados , Feminino , Acessibilidade aos Serviços de Saúde , Humanos , Gravidez , Adulto Jovem , ZâmbiaRESUMO
Rolling circle replication from M13 DNA circles was previously reconstituted in vitro using purified factors encoded by bacteriophage T4. The products are duplex circles with linear tails >100 kb. When T4 DNA polymerase deficient in 3' to 5' exonuclease activity was employed, electron microscopy revealed short single-stranded DNA "flaps" along the replicated tails. This marked the beginning of each Okazaki fragment, allowing an analysis of the lengths of sequential Okazaki fragments on individual replicating molecules. DNAs containing runs of Okazaki fragments of similar length were found, but most showed large length variations over runs of six or more fragments reflecting the broad population distribution.
Assuntos
Bacteriófago M13/genética , Bacteriófago T4/genética , Bacteriófago T4/metabolismo , Replicação do DNA , DNA de Cadeia Simples/genética , DNA Polimerase Dirigida por DNA/metabolismo , DNA/genética , Exodesoxirribonucleases/metabolismo , Proteínas Virais/metabolismo , DNA/química , DNA Helicases/metabolismo , DNA de Cadeia Simples/química , DNA de Cadeia Simples/ultraestrutura , DNA Viral/química , DNA Viral/genética , DNA Viral/ultraestrutura , DNA Polimerase Dirigida por DNA/genética , Escherichia coli/genética , Escherichia coli/virologia , Exodesoxirribonuclease V , Exodesoxirribonucleases/genética , Deleção de SequênciaRESUMO
A novel DNA structure, sticky DNA, is described for lengths of (GAA.TTC)n found in intron 1 of the frataxin gene of Friedreich's ataxia patients. Sticky DNA is formed by the association of two purine.purine.pyrimidine (R.R.Y) triplexes in negatively supercoiled plasmids at neutral pH. An excellent correlation was found between the lengths of (GAA.TTC) (> 59 repeats): first, in FRDA patients, second, required to inhibit transcription in vivo and in vitro, and third, required to adopt the sticky conformation. Fourth, (GAAGGA.TCCTTC)65, also found in intron 1, does not form sticky DNA, inhibit transcription, or associate with the disease. Hence, R.R.Y triplexes and/or sticky DNA may be involved in the etiology of FRDA.
Assuntos
DNA/genética , Ataxia de Friedreich/genética , Proteínas de Ligação ao Ferro , Fosfotransferases (Aceptor do Grupo Álcool)/genética , DNA/ultraestrutura , DNA Super-Helicoidal/genética , DNA Super-Helicoidal/ultraestrutura , Eletroforese em Gel de Ágar , Ataxia de Friedreich/etiologia , Humanos , Microscopia Eletrônica , Modelos Moleculares , Conformação de Ácido Nucleico , Sequências Repetitivas de Ácido Nucleico , Transcrição Gênica , FrataxinaRESUMO
The coordinated synthesis of both leading and lagging DNA strands is thought to involve a dimeric DNA polymerase and a looping of the lagging strand so that both strands can be synthesized in the same direction. We have constructed a minicircle with a replication fork that permits an assessment of the stoichiometry of the proteins and a measurement of the synthesis of each strand. The replisome consisting of bacteriophage T7 DNA polymerase, helicase, primase, and single-stranded DNA-binding protein mediates coordinated replication. The criteria for coordination are fulfilled: (1) a replication loop is formed, (2) leading and lagging strand synthesis are coupled, (3) the lagging strand polymerase recycles from one Okazaki fragment to another, and (4) the length of Okazaki fragments is regulated. T7 single-stranded DNA-binding protein is essential for coordination.
Assuntos
Replicação do DNA , DNA Circular/metabolismo , DNA/biossíntese , Bacteriófago T7/química , Bacteriófago T7/enzimologia , Bacteriófago T7/genética , Sequência de Bases , Sítios de Ligação/genética , DNA/química , DNA/metabolismo , DNA/ultraestrutura , DNA Helicases/metabolismo , DNA Polimerase III/metabolismo , DNA Primase/metabolismo , DNA Circular/química , DNA Circular/ultraestrutura , DNA de Cadeia Simples/química , DNA de Cadeia Simples/metabolismo , DNA Viral/química , DNA Viral/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Moldes GenéticosRESUMO
The lengthening of tracts of CTG, CGG and GAA triplet repeats during progression of a pedigree has been associated with more than 12 human genetic diseases, including fragile X syndrome, myotonic dystrophy and Friedreich's ataxia. These repetitive sequence elements have the potential to form alternative DNA secondary structures that may contribute to their instability. The alternative DNA secondary structures may mediate errors during DNA replication, repair or recombination of the triplet repeat, leading to expansion. Here we show that DNA composed of pure CTG or CGG repeats exhibits anomalously fast mobility on polyacrylamide gels, confirming a previous observation for DNA containing CTG and CGG triplet repeats flanked by mixed sequence DNA. Moreover, we show that even short tracts of duplex CTG repeats have an unusual helix structure. CTG repeats reduce overall curvature associated with phased A-tract or GGCC curves, but alone they do not introduce curvature into DNA. The reduction in curvature of phased A-tracts by CTG repeats is similar to that afforded by an interspersed flexible region associated with a (TT).(TT) mispair. CTG-containing DNAs exhibit a rapid rate of cyclization, consistent with a flexible helix. These results suggest that tracts of (CTG).(CAG) repeats are inherently flexible. In addition, our results suggest that the unusual rapid electrophoretic mobility of CTG or CGG-containing DNA may be a consequence of an extended flexible DNA chain.
Assuntos
Doenças Genéticas Inatas/genética , Doenças do Sistema Nervoso/genética , Conformação de Ácido Nucleico , Repetições de Trinucleotídeos/genética , Citosina , Guanina , Humanos , TiminaRESUMO
Eight human genetic diseases have been associated with the expansion of CTG or CGG triplet repeats. The molecular etiology behind expansion is unknown but may involve participation of an unusual DNA structure in replication, repair, or recombination. We show that DNA fragments containing CTG triplet repeats derived from the human myotonic dystrophy gene migrate up to 20% faster than expected in nondenaturing polyacrylamide gels, suggesting the presence of an unusual DNA helix structure within the CTG triplet repeats. The anomalous migration is dependent upon the number of triplet repeats, the length of the flanking DNA, and the percentage and temperature of the polyacrylamide. The effect could be reduced by the addition of actinomycin D. Applying a reptation model for electrophoresis, the results are consistent with a 20% increase in persistence length of the DNA. PCR products containing CTG or CGG repeats from the spinocerebellar ataxia type I gene (SCA1) or the fragile X FMR1 gene, respectively, also showed higher electrophoretic mobility. These are the first sequences of defined length for which a dramatic increase in mobility can be attributed to sequence-dependent structural elements in DNA.
Assuntos
DNA/química , Síndrome do Cromossomo X Frágil/genética , Sequências Repetitivas de Ácido Nucleico , Degenerações Espinocerebelares/genética , Ataxina-1 , Ataxinas , Sequência de Bases , DNA/genética , DNA/isolamento & purificação , Dactinomicina/farmacologia , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Proteína do X Frágil da Deficiência Intelectual , Humanos , Dados de Sequência Molecular , Distrofia Miotônica/genética , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , Proteínas de Ligação a RNA/genética , Mapeamento por RestriçãoRESUMO
The current practice of physical therapy rarely addresses the needs of patients with psychiatric disorders. The literature has shown that physical and psychological health are related intimately. This article describes a physical fitness training program designed for inpatients with mental illness in an acute care hospital setting. A training program consisting of group exercise sessions conducted three times a week for six weeks may enhance the patients' self-esteem and body image in a unique way. Physical therapy intervention should be broadened to address the special needs of the mentally disabled.
Assuntos
Terapia por Exercício , Transtornos Mentais/reabilitação , Aptidão Física , Adolescente , Adulto , Imagem Corporal , Emoções , Feminino , Humanos , Masculino , Transtornos Mentais/psicologia , Pessoa de Meia-Idade , Testes Psicológicos , AutoimagemRESUMO
The effect of a 20-minute treatment of short-wave diathermy on isometric strength of the quadriceps muscle group was studied in 12 subjects over a 2-hour period. The heat treatment caused an initial decrease in isometric strength followed by an increase 30 minutes after the heat was terminated. Strength remained significantly above the control level for two hours following heat. The findings of this study suggest that the relationship between the application of short wave diathermy and the optimal time which should elapse between the heat treatment and therapeutic exercise should be examined.
