Evidência Sorológica e molecular de hantavirus em pacientes com síndrome febril agudo atendido no centro de saúde polana caniço durante o período de 2012-2014 / Serological and molecular evidence of hantavirus in patients with acute febrile syndrome seen at the caniço health center during the period 2012-2014

Hantavírus são vírus RNA pertencentes à família Bunyaviridae transmitidos por roedores silvestres de diferentes espécies. Em humanos, os hantavírus estão associados a duas síndromes clínicas: a febre hemorrágica com síndrome renal, descrita na Europa, África e Ásia e a síndrome pulmonar por hantavírus (SPH) também reconhecida como a síndrome cardiopulmonar por hantavírus (SCPH), doença emergente com descrição crescente de casos no continente americano.Na África subsaariana, a ocorrência de hantavírus é pouco estudada e a sua implicação clínica é desconhecida. O estudo teve como objectivo determinar a ocorrência de infecção por hantavírus em pacientes febris atendidos em consulta ambulatória em um centro de atenção primária, localizada em uma área suburbana da cidade de Maputo, em Moçambique. Um total de 200 pacientes febris com idade> 5 anos foram recrutados entre Fevereiro de 2012 e Outubro de 2014. De cada paciente, uma amostra de sangue foi colectada na fase aguda e convalescente para análise serológica utilizando proteína recombinante Andes. Anticorpos anti-hantavírus IgM e IgG foram detectados em 4(2,0%) dos pacientes incluídos no estudo e todas as amostras IgM reactivas submetidas à RT-PCR foram negativas

Hantavirus are RNA viruses belonging to the Bunyaviridae family transmitted by wild rodents of different species. In humans, hantaviruses are associated with two clinical syndromes: hemorrhagic fever with renal syndrome, described in Europe, Africa, and Asia, and hantavirus pulmonary syndrome (HPS) also recognized as hantavirus cardiopulmonary syndrome (SCPH), an emerging disease with An increasing description of cases in the American continent. In sub-Saharan Africa, the occurrence of hantavirus is poorly studied and its clinical implication unknown. The objective of the study was to determine the occurrence of hantavirus infection in febrile patients seen at an outpatient clinic in a primary care center located in a suburban area of the city of Maputo, Mozambique. A total of 200 febrile patients aged> 5 years were recruited between February 2012 and October 2014. From each patient, a blood sample was collected in the acute and convalescent phase for serological analysis using Andes recombinant protein. IgM and IgG anti-hantavirus antibodies were detected in 4 (2.0%) of the patients included in the study and all reactive IgM samples submitted to RT-PCR were negative.

First serological evidence of hantavirus among febrile patients in Mozambique.

OBJECTIVE: The occurrence of hantavirus in Sub-Saharan Africa is poorly studied and its clinical implications are unknown. This study aimed to determine the occurrence of hantavirus infection among febrile patients attending an outpatient clinic at a primary health care center located in a suburban area of the city of Maputo in Mozambique. METHODS: Paired acute and convalescent samples from a total of 200 febrile patients aged >5years who were recruited between February 2012 and October 2014 were screened for IgM and IgG antibodies against hantavirus using an ELISA. Acute samples were also screened for malaria and to determine hematological and clinical chemistry parameters. RESULTS: Of the 200 patients enrolled, four had IgM antibodies in their acute sample and IgG antibodies in their convalescent sample, yielding a prevalence rate of 2%. Contact with rodents was higher among IgM-positive participants than IgM-negative participants (50.0% (2/4) vs. 15.3% (30/196)). IgM-positive patients presented significantly higher levels of creatinine and alanine aminotransferase and lower platelet counts than IgM-negative patients. CONCLUSIONS: The findings of this study demonstrate human exposure to hantavirus in Mozambique for the first time; however, further studies should be conducted to investigate its clinical implications.

First serological evidence of hantavirus among febrile patients in Mozambique Ramalho

Objective: The occurrence of hantavirus in Sub-Saharan Africa is poorly studied and its clinical implications are unknown. This study aimed to determine the occurrence of hantavirus infection among febrile patients attending an outpatient clinic at a primary health care center located in a suburban area of the city of Maputo in Mozambique. Methods: Paired acute and convalescent samples from a total of 200 febrile patients aged >5 years who were recruited between February 2012 and October 2014 were screened for IgM and IgG antibodies against hantavirus using an ELISA. Acute samples were also screened for malaria and to determine hematological and clinical chemistry parameters. Results: Of the 200 patients enrolled, four had IgM antibodies in their acute sample and IgG antibodies in their convalescent sample, yielding a prevalence rate of 2%. Contact with rodents was higher among IgM-positive participants than IgM-negative participants (50.0% (2/4) vs. 15.3% (30/196)). IgM-positive patients presented significantly higher levels of creatinine and alanine aminotransferase and lower platelet counts than IgM-negative patients. Conclusions: The findings of this study demonstrate human exposure to hantavirus in Mozambique for the first time; however, further studies should be conducted to investigate its clinical implications. © 2017 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-