The Combination of Citrus Rootstock and Scion Cultivar Influences Trioza erytreae (Hemiptera: Triozidae) Survival, Preference Choice and Oviposition.

Trioza erytreae (Del Guercio, 1918) (Hemiptera: Triozidae) is a citrus pest which produces gall symptoms on leaves and transmits bacteria associated with the citrus disease Huanglongbing, 'Candidatus Liberibacter' spp. In the present work, the biology and behaviour of T. erytreae were studied in different rootstock-cultivar combinations. Six rootstocks were used, Flying dragon (FD), 'Cleopatra' mandarin (CL), Carrizo citrange (CC), Forner-Alcaide no.5 (FA5), Forner-Alcaide no.517 (FA517) and Citrus macrophylla (CM), and six scion cultivars: 'Star Ruby', 'Clemenules', 'Navelina', 'Valencia Late', 'Fino 49' and 'Ortanique'. Survival and oviposition were evaluated in a no-choice trial, and preference in a choice trial, all of them under greenhouse conditions. Trioza erytreae did not show a clear settle preference for any citrus combination. However, it was able to lay more eggs in 'Fino 49' grafted on CC than on FD. In terms of survival, 'Ortanique' grafted onto FA5 was more suitable than when grafted onto FA517, and in the case of 'Valencia Late', when it was grafted onto CM rather than CC. Our results showed that T. erytreae behave differently depending on the citrus combination.

Cardiac hypertrophy that affects hyperthyroidism occurs independently of the NLRP3 inflammasome.

Cardiac hypertrophy (CH) is an adaptive response to maintain cardiac function; however, persistent stress responses lead to contractile dysfunction and heart failure. Although inflammation is involved in these processes, the mechanisms that control cardiac inflammation and hypertrophy still need to be clarified. The NLRP3 inflammasome is a cytosolic multiprotein complex that mediates IL-1ß production. The priming step of NLRP3 is essential for increasing the expression of its components and occurs following NF-κB activation. Hyperthyroidism triggers CH, which can progress to maladaptive CH and even heart failure. We have shown in a previous study that thyroid hormone (TH)-induced CH is linked to the upregulation of S100A8, leading to NF-κB activation. Therefore, we aimed to investigate whether the NLRP3 inflammasome is involved in TH-induced CH and its potential role in CH pathophysiology. Hyperthyroidism was induced in NLRP3 knockout (NLRP3-KO), Caspase-1-KO and Wild Type (WT) male mice of the C57Bl/6J strain, aged 8-12 weeks, by triiodothyronine (7 µg/100 g BW, i.p.) administered daily for 14 days. Morphological and cardiac functional analysis besides molecular assays showed, for the first time, that TH-induced CH is accompanied by reduced NLRP3 expression in the heart and that it occurs independently of the NLRP3 inflammasome and caspase 1-related pathways. However, NLRP3 is important for the maintenance of basal cardiac function since NLRP3-KO mice had impaired diastolic function and reduced heart rate, ejection fraction, and fractional shortening compared with WT mice.

Complete genome sequences generated using hybrid Nanopore-Illumina assembly of two non-typical Avibacterium paragallinarum strains isolated from clinically normal chicken flocks.

We report the complete genome sequences of two non-typical Avibacterium paragallinarum (AP) strains isolated from chickens in the absence of clinical signs. The availability of these genomes can aid scientists in improving current diagnostics and increase our understanding of AP epidemiology and pathogenicity in chickens.

Thermal Gelation of Proteins from Cajanus cajan Influenced by pH and Ionic Strength.

Cajanus cajan [pigeon pea (PP)] is an important legume crop for subsistence agriculture and its seeds are an alternative plant-based protein source. PP protein isolates (PPI) are able to form heat-induced gels that could be used for food applications. The aim of this work was to study the influence of pH (2.1, 3.9, 6.3, and 8.3) and ionic strength (µ) (0.10 and 0.54) on thermal stability and thermal gelation of PPI obtained by alkaline extraction (pH 8.0) and isoelectric precipitation. Thermal stability of PPI changed with pH variation at low ionic strength (µ = 0.10), decreasing this dependence with the increase of ionic strength (µ = 0.54). At µ = 0.10, gelation capacity of PPI was lower at pH 2.1 and pH 3.9. These gels presented a coarse network, which entails low WHC. At pH 6.3 and pH 8.3, gels showed a solid-like character with a compact and homogeneous matrix, with better WHC. At µ = 0.54, gel formation was favoured at pH 2.1 and pH 3.9. G'20/G'95 ratio values and differential solubility results suggest that hydrogen bonds and electrostatic interactions could play an important role in gel formation at pH 6.3 and pH 8.3.

Clínicas Integradas em Odontologia, desafios, oportunidades e perspectivas: uma revisão de literatura

As clínicas integradas em Odontologia constituem em um desafio para o ensino superior na formação do profissional, que deverá aprender a tratar as condições orofaciais de seu paciente de forma diferenciada no futuro próximo, aliando cada vez mais a Odontologia e as novas tecnologias. Objetivou-se com este estudo de revisão discutida entender o conceito de clínica integrada e suas perspectivas em consonância com as Diretrizes Curriculares Nacionais. Foi realizado um levantamento bibliográfico sistematizado nos bancos de dados eletrônicos: Lilacs, PubMed e Scielo. Os termos em português e inglês "Educação em Odontologia" (Dental Education), "Clínicas Odontológicas" (Dental Clinics), "Graduação" (Undergraduate Program), "Prestação Integrada de Cuidados de Saúde" (delivery of health care, integrated) e "Currículo" (Curriculum) foram utilizados como palavras-chave. Foram eleitos 23 artigos para esta revisão e extraídas as ideias principais dos textos tendo como base as metodologias utilizadas e os principais resultados. Os autores foram unânimes em ressaltar que as clínicas integradas em Odontologia exercem influência na formação e no preparo do egresso generalista através do treinamento sobre um planejamento global, inter e multidisciplinar como pilar essencial para um currículo integrativo e inovador.

ntegrated clinics in Dentistry present a challenge for higher education in the training of professionals, who must adapt their approach to treating orofacial conditions in the near future, increasingly incorporating Dentistry and new technologies. This review study aimed to comprehend the concept of integrated clinics and its alignment with the National Curricular Guidelines. A systematic bibliographic survey was conducted in the following electronic databases: Lilacs, PubMed, and Scielo. The search employed keywords in both Portuguese and English, including "Education in Dentistry" (Dental Education), "Dental Clinics", "Graduation" (undergraduate program), "Integrated Provision of Health Care" (delivery of health care, integrated), and "Curriculum." Approximately 23 articles were selected for this review, and the primary ideas within the texts were extracted based on the employed methodologies and the principal results. The authors unanimously emphasized that integrated dentistry clinics significantly impact the training and preparation of generalist graduates. This influence stems from emphasizing global, inter, and multidisciplinary planning as an indispensable foundation for an integrative and innovative curriculum.

Development of a Clinical Score to Stratify the Risk for Carbapenem-Resistant Enterobacterales Bacteremia in Patients with Cancer and Hematopoietic Stem Cell Transplantation.

Identifying the risk factors for carbapenem-resistant Enterobacterales (CRE) bacteremia in cancer and hematopoietic stem cell transplantation (HSCT) patients would allow earlier initiation of an appropriate empirical antibiotic treatment. This is a prospective multicenter observational study in patients from 12 centers in Argentina, who presented with cancer or hematopoietic stem-cell transplant and developed Enterobacterales bacteremia. A multiple logistic regression model identified risk factors for CRE bacteremia, and a score was developed according to the regression coefficient. This was validated by the bootstrap resampling technique. Four hundred and forty-three patients with Enterobacterales bacteremia were included: 59 with CRE and 384 with carbapenem-susceptible Enterobacterales (CSE). The risk factors that were identified and the points assigned to each of them were: ≥10 days of hospitalization until bacteremia: OR 4.03, 95% CI 1.88-8.66 (2 points); previous antibiotics > 7 days: OR 4.65, 95% CI 2.29-9.46 (2 points); current colonization with KPC-carbapenemase-producing Enterobacterales: 33.08, 95% CI 11.74-93.25 (5 points). With a cut-off of 7 points, a sensitivity of 35.59%, specificity of 98.43%, PPV of 77.7%, and NPV of 90.9% were obtained. The overall performance of the score was satisfactory (AUROC of 0.85, 95% CI 0.80-0.91). Finally, the post-test probability of CRE occurrence in patients with none of the risk factors was 1.9%, which would virtually rule out the presence of CRE bacteremia.

A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus 'Candidatus Liberibacter' in plants and insects.

Four pathogenic bacterial species of the genus 'Candidatus Liberibacter', transmitted by psyllid vectors, have been associated with serious diseases affecting economically important crops of Rutaceae, Apiaceae and Solanaceae families. The most severe disease of citrus plants, huanglongbing (HLB), is associated with 'Ca. Liberibacter asiaticus' (CaLas), 'Ca. Liberibacter americanus' (CaLam) and 'Ca. Liberibacter africanus' (CaLaf), while 'Ca. Liberibacter solanacearum' (CaLsol) is associated with zebra chip disease in potatoes and vegetative disorders in apiaceous plants. Since these bacteria remain non-culturable and their symptoms are non-specific, their detection and identification are done by molecular methods, mainly based on PCR protocols. In this study, a new quantitative real-time PCR protocol based on TaqMan probe, which can also be performed in a conventional PCR version, has been developed to detect the four known phytopathogenic species of the genus Liberibacter. The new protocol has been validated according to European Plant Protection Organization (EPPO) guidelines and is able to detect CaLas, CaLam, CaLaf and CaLsol in both plants and vectors, not only using purified DNA but also using crude extracts of potato and citrus or psyllids. A comparative analysis with other previously described qPCR protocols revealed that this new one developed in this study is more specific and equally or more sensitive. Thus, other genus-specific qPCR protocols have important drawbacks regarding the lack of specificity, while with the new protocol there was no cross-reactions in 250 samples from 24 different plant and insect species from eight different geographical origins. Therefore, it can be used as a rapid and time-saving screening test, as it allows simultaneous detection of all plant pathogenic species of 'Ca. Liberibacter' in a one-step assay.

Metabolic Background, Not Photosynthetic Physiology, Determines Drought and Drought Recovery Responses in C3 and C2 Moricandias.

Distinct photosynthetic physiologies are found within the Moricandia genus, both C3-type and C2-type representatives being known. As C2-physiology is an adaptation to drier environments, a study of physiology, biochemistry and transcriptomics was conducted to investigate whether plants with C2-physiology are more tolerant of low water availability and recover better from drought. Our data on Moricandia moricandioides (Mmo, C3), M. arvensis (Mav, C2) and M. suffruticosa (Msu, C2) show that C3 and C2-type Moricandias are metabolically distinct under all conditions tested (well-watered, severe drought, early drought recovery). Photosynthetic activity was found to be largely dependent upon the stomatal opening. The C2-type M. arvensis was able to secure 25-50% of photosynthesis under severe drought as compared to the C3-type M. moricandioides. Nevertheless, the C2-physiology does not seem to play a central role in M. arvensis drought responses and drought recovery. Instead, our biochemical data indicated metabolic differences in carbon and redox-related metabolism under the examined conditions. The cell wall dynamics and glucosinolate metabolism regulations were found to be major discriminators between M. arvensis and M. moricandioides at the transcription level.

Ablation of miRNA-22 protects against obesity-induced adipocyte senescence and ameliorates metabolic disorders in middle-aged mice.

High-fat diet (HFD) promotes obesity-related metabolic complications by activating cellular senescence in white adipose tissue (WAT). Growing evidence supports the importance of microRNA-22 (miR-22) in metabolic disorders and cellular senescence. Recently, we showed that miR-22 deletion attenuates obesity-related metabolic abnormalities. However, whether miR-22 mediates HFD-induced cellular senescence of WAT remains unknown. Here, we uncovered that obese mice displayed increased pri-miR-22 levels and cellular senescence in WAT. However, miR-22 ablation protected mice against HFD-induced WAT senescence. In addition, in vitro studies showed that miR-22 deletion prevented preadipocyte senescence in response to Doxorubicin (Doxo). Loss-of-function studies in vitro and in vivo revealed that miR-22 increases H2ax mRNA and γH2ax levels in preadipocytes and WAT without inducing DNA damage. Intriguingly, miR-22 ablation prevented HFD-induced increase in γH2ax levels and DNA damage in WAT. Similarly, miR-22 deletion prevented Doxo-induced increase in γH2ax levels in preadipocytes. Adipose miR-22 levels were enhanced in middle-aged mice fed a HFD than those found in young mice. Furthermore, miR-22 deletion attenuated fat mass gain and glucose imbalance induced by HFD in middle-aged mice. Overall, our findings indicate that miR-22 is a key regulator of obesity-induced WAT senescence and metabolic disorders in middle-aged mice.

miRNA-22 is involved in the aortic reactivity in physiological conditions and mediates obesity-induced perivascular adipose tissue dysfunction.

AIMS: Blood vessels are surrounded by perivascular adipose tissue (PVAT), which plays an important role in vascular tonus regulation due to its anticontractile effect; however, this effect is impaired in obesity. We previously demonstrated that miRNA-22 is involved in obesity-related metabolic disorders. However, the impact of miRNA-22 on vascular reactivity and PVAT function is unknown. AIM: To investigate the role of miRNA-22 on vascular reactivity and its impact on obesity-induced PVAT dysfunction. MAIN METHODS: Wild-type and miRNA-22 knockout (KO) mice were fed a control or a high-fat (HF) diet. To characterize the vascular response, concentration-responses curves to noradrenaline were performed in PVAT- or PVAT+ thoracic aortic rings in absence and presence of L-NAME. Expression of adipogenic and thermogenic markers and NOS isoforms were evaluated by western blotting or qPCR. KEY FINDINGS: HF diet and miRNA-22 deletion reduced noradrenaline-induced contraction in PVAT- aortic rings. Additionally, miRNA-22 deletion increased noradrenaline-induced contraction in PVAT+ aortic rings without affecting its sensitivity; however, this effect was not observed in miRNA-22 KO mice fed a HF diet. Interestingly, miRNA-22 deletion reduced the contraction of aortic rings to noradrenaline via a NOS-dependent mechanism. Moreover, HF diet abolished the NOS-mediated anticontractile effect of PVAT, which was attenuated by miRNA-22 deletion. Mechanistically, we found that PVAT from miRNA-22 KO mice fed a HF diet presented increased protein expression of nNOS. SIGNIFICANCE: These results suggest that miRNA-22 is important for aorta reactivity under physiological circumstances and its deletion attenuates the loss of the NOS-mediated anticontractile effect of PVAT in obesity.

Reflexões acerca dos métodos mistos na perspectiva da Teoria de Intervenção Práxica de Enfermagem em Saúde Coletiva / Reflections on mixed methods from the perspective of the theory of praxis intervention of nursing in collective health / Reflexiones sobre los métodos mixtos desde la perspectiva de la teoría de la intervención de la praxis en enfermería de salud pública

Objetivo: este estudo tem o objetivo de apresentar reflexões sobre o desenvolvimento de um estudo metodologicamente elaborado com métodos mistos na perspectiva teórica da Enfermagem em saúde coletiva. Tem como base filosófica o Materialismo Histórico e Dialético. O objeto do estudo foi a Determinação Social das Internações por Condições Sensíveis à Atenção Primária de adolescentes em uma regional de saúde de um estado do Sul do Brasil. Método: estudo descritivo e do tipo reflexivo, subdivido em três momentos. O primeiro trata da fundamentação teórica e metodológica, com diálogos entre os pressupostos dos métodos mistos e a Teoria de Intervenção Práxica da Enfermagem em Saúde Coletiva. No segundo momento, é descrita a seleção dos procedimentos para a coleta de dados quantitativos e qualitativos, evidenciado pela necessidade de definir a combinação desses dados na sequência do processo, assim como estabelecer os pesos dos dados no desenvolvimento da análise do fenômeno. No terceiro momento, reflete-se sobre a integração de dados, destacando as combinações das informações que ocorreram na etapa anterior, conforme referenda o próprio método. Resultado: o estudo desenvolvido por meio de métodos misto potencializou uma análise crítica que, ancorada nos pressupostos da Enfermagem em Saúde Coletiva, permitiu compreender as conexões entre os dados quantitativos e qualitativos presentes na historicidade e na dinamicidade do objeto. Considerações finais: destaca-se o ineditismo da interseção desses campos teóricos e metodológicos para compreender o fenômeno e propor ações efetivas para o enfrentamento dos processos que o determinam na sua realidade objetiva.(AU)

Objective: This study aims to present reflections on the development of a methodologically elaborated study with mixed methods in the theoretical perspective of Nursing in collective health. Its philosophical basis is Historical and Dialectical Materialism. The object of the study was the Social Determination of hospitalizations due to Ambulatory-Care Sensitive Conditions of adolescents in a health regional in a southern state of Brazil. Method: descriptive and reflective study, subdivided into three moments. The first deals with the theoretical and methodological foundations, with dialogues between the assumptions of mixed methods and the Theory of Praxis Intervention of Nursing in Public Health. In the second moment, the selection of procedures for the collection of quantitative and qualitative data is described, evidenced by the need to define the combination of these data in the sequence of the process, as well as to establish the weights of the data in the development of the analysis of the phenomenon. In the third moment, the integration of data is reflected, highlighting the combinations of information that occurred in the previous steps, as referred to by the method itself. Result: the study developed using mixed methods enhanced a critical analysis that, anchored in the assumptions of Public Health Nursing, allowed understanding the connections between the quantitative and qualitative data present in the historicity and dynamics of the object. Final considerations:the unprecedented intersection of these theoretical and methodological fields stands out to understand the phenomenon and propose effective actions to face the processes that determine it in its objective reality.(AU)

Objetivo: este estudio tiene como objetivo presentar reflexiones sobre el desarrollo em un estudio metodológicamente elaborado con métodos mixtem en la perspectiva teórica de la Enfermería de salud pública, que tiene como base filosófica el Materialismo Histórico y Dialéctico. El objeto de estudio fue la Determinación Social de Hospitalizaciones por Condiciones Sensibles a la Atención Primaria de adolescentem em un departamento regional de salud em un estado del sur de Brasil. Método: estudio descriptivo y del tipo reflexivo, subdiviem en tres momentos, el primero de los cuales trata de la fundamentación teórica y metodológica con diálogos entre las premisas de los métodos mixtos y la Teoría de la Intervención Práctica de Enfermerem en Salud Pública. El segundo momento describe la selección de procedimientos para la recolección de datos cuantitativos y cualitativos, evidenciada por la necesidad de definir la combinación de este en la secuencia del proceso, así como establecer los pesos de los datem en el desarrollo del análisis del fenómeno. El tercer momento reflexiona sobre la integración de los datos, destacando las combinaciones de informaciones ocurridas en el paso anterior, referenciadas por empio método. Resultados: el estudio desarrollado a través de métodos mixtos potenció el análisis crítico que, anclaem en los presupuestos de la enfermería de salud colectiva, permitió comprender las conexiones entre los datos cuantitativos y cualitativos presentes en la historicidad y dinamismo del objeto. Conclusión: destaca la novedad de la intersección de estos campos teóricos y metodológicos para comprender el fenómeno y proponer acciones eficaces para abordar los procesos que lo determinem en su realidad objetiva.(AU)

Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis.

Colorectal cancer is the third most diagnosed cancer worldwide and linked to dietary/lifestyle factors. Arthrospira (Spirulina) platensis (AP) contains bioactive compounds with beneficial effects in vivo/in vitro. We evaluated the effects of AP feeding against 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Male Sprague Dawley rats were given subcutaneous injections of DMH (4 × 40 mg/kg body weight) (G1-G3) or vehicle (G4-G5) twice a week (weeks 3-4). During weeks 1-4, animals were fed a diet containing 1 % (G2) or 2 % (G3-G4) AP powder (w/w). After this period, all groups received a balanced diet until week 12. Some animals were euthanised after the last DMH injection (week 4) for histological, immunohistochemical (Ki-67, γ-H2AX and caspase-3) and molecular analyses (real time-PCR for 91 genes), while other animals were euthanised at week 12 for preneoplastic aberrant crypt foci (ACF) analysis. Both AP treatments (G2-G3) significantly decreased the DMH-induced increase in γ-H2AX (DNA damage) and caspase 3 (DNA damage-induced cell death) in colonic crypts at week 4. In addition, Cyp2e1 (Drug metabolism), Notch1, Notch2 and Jag1 genes (Notch pathway) and Atm, Wee1, Chek2, Mgmt, Ogg1 and Xrcc6 genes (DNA repair) were also down-regulated by 2 % AP feeding (G3) at week 4. A significant reduction in ACF development was observed in both AP-treated groups (G2-G3) at week 12. In conclusion, findings indicate that AP feeding reduced acute colonic damage after DMH, resulting in fewer preneoplastic lesions. Our study provided mechanistic insights on dietary AP-preventive effects against early colon carcinogenesis.

Internações de adolescentes por condições sensíveis à atenção primária à saúde na perspectiva da integralidade / Hospitalizaciones de adolescentes por condiciones sensibles de la atención primaria de salud desde la perspectiva de la integralidad / Hospitalizations of adolescents due to sensitive conditions in primary health care in the perspective of integrality

Resumo Objetivo analisar, na perspectiva da Integralidade, as internações de adolescentes por condições sensíveis à Atenção Primária em uma Regional de Saúde do Paraná. Método estudo de abordagem quantitativa, retrospectivo, realizado no período de janeiro a julho de 2018. Foram coletados dados públicos de internações de adolescentes de 10 a 19 anos disponíveis na base de dados do sistema de informações hospitalares do Ministério da Saúde, com Tabulador Oficial versão Tabwin 3.2. A análise dos dados ocorreu por estatística descritiva segundo número absoluto e frequência por ano investigado. Resultados das 82.016 internações, 9.029 (11,00%) foram por condições sensíveis à atenção primária. Entre as principais causas, destacam-se a infecção do rim e trato urinário (24,96%); epilepsias (19,27%); gastroenterites infecciosas e complicações (11,91%); doenças relacionadas ao pré-natal e parto (8,88%) e asma (7,39%). As internações do sexo feminino representaram 57,52%, prevalentes no subgrupo da faixa etária de 15 a 19 anos (66,64%). Conclusão e implicações para a prática é necessário avançar na perspectiva da construção da integralidade na atenção à saúde do adolescente, para responder às necessidades em saúde deste segmento populacional e reduzir hospitalizações por causas sensíveis à atenção primária.

Resumen Objetivo analizar, en la perspectiva de la Integralidad, las hospitalizaciones de adolescentes por condiciones sensibles a la Atención Primaria en una Regional de Salud de Paraná. Método estudio retrospectivo cuantitativo realizado de enero a julio de 2018. Se recolectaron datos públicos de hospitalizaciones de adolescentes de 10 a 19 años disponibles en la base de datos del sistema de información hospitalario del Ministerio de Salud, con Tabulador Oficial Tabwin versión 3.2. El análisis de los datos se realizó mediante estadística descriptiva según número absoluto y frecuencia por año investigado. Resultados de los 82.016 ingresos, 9.029 (11,00%) se debieron a condiciones sensibles a la atención primaria. Entre las principales causas destaca la infección del riñón y vías urinarias (24,96%); epilepsias (19,27%); gastroenteritis infecciosa y complicaciones (11,91%); enfermedades relacionadas con la atención prenatal y el parto (8,88%) y asma (7,39%). Las hospitalizaciones femeninas representaron el 57,52%, con prevalencia en el subgrupo de 15 a 19 años (66,64%). Conclusión e implicaciones para la práctica es necesario avanzar en la perspectiva de la construcción de la integralidad en la atención a la salud del adolescente, a fin de responder a las necesidades de salud de este segmento poblacional y reducir las hospitalizaciones por causas sensibles a la atención primaria.

Abstract Objective to analyze, from the perspective of integrality, the hospitalizations of adolescents due to conditions sensitive to primary care in a Regional Health unit in Paraná State. Method a quantitative retrospective study was carried out from January to July 2018. Public data were collected from hospitalizations of adolescents aged 10 to 19 years available in the database of the hospital information system of the Ministry of Health using the Official Tabulator (Tabwin, version 3.2). Data analysis was performed using descriptive statistics according to the absolute number and frequency per year investigated. Results out of the 82,016 admissions, 9,029 (11.00%) were due to conditions sensitive to primary care. Among the main causes, infection of the kidney and urinary tract (24.96%), epilepsies (19.27%), infectious gastroenteritis, and complications (11.91%) stood out, which are diseases related to prenatal care and childbirth (8.88%) and asthma (7.39%). Female hospitalizations accounted for 57.52%, prevalent in the 15- to 19-year-old subgroup (66.64%). Conclusion and implications for practice it is necessary to advance in the construction of integrality in adolescent health care to respond to the health needs of this population segment and reduce hospitalizations for causes sensitive to primary care.

Experiência de extensão universitária em serviço social na Amazônia / Experience of university extension in social work in the Amazon

Este artigo relata a experiência de extensão universitária do Grupo Interdisciplinar de Estudos Socioambientais e de Desenvolvimento de Tecnologias Sociais, da Universidade Federal do Amazonas (UFAM), no "Programa Inter-Ação: práticas de sustentabilidade visando o desenvolvimento regional", no meio urbano e junto a povos e comunidades tradicionais da Amazônia. Os procedimentos metodológicos, baseados no Método Interação (modalidade de pesquisa-ação), abrangem um conjunto de técnicas e instrumentais de coleta de dados: observação participante, grupo focal, dinâmicas de abordagem grupal e outras. O resultado das ações de extensão permitiu: formar competências dinâmicas compromissadas em atuar pelo protagonismo social dos povos amazônidas; fortalecer as organizações dos grupos sociais; disponibilizar subsídios para instituições de políticas públicas com proposições coerentes com os interesses e necessidades dos grupos sociais da região

This article reports the experience of university extension of the Interdisciplinary Group of Socio-environmental Studies and Development of Social Technologies, of the Federal University of Amazonas, in the "Inter-Action Program: sustainability practices aiming at regional development", in the urban environment and with the people and traditional communities in the Amazon. The methodological procedures, based on the Interaction Method (action-research modality), cover a set of data collection techniques and instruments: participant observation, focus group, dynamics of group approach, and others. The result of the extension actions allowed: to form dynamic competences committed to acting for the social protagonism of the Amazonian peoples; strengthen the organizations of social groups; provide subsidies for public policy institutions with propositions that are coherent with the interests and needs of social groups in the region

Desafios terapêuticos da mucosite oral em oncopediatria / Therapeutic challenges of oral mucositis in pediatric oncology

O Neuroblastoma (NB) é uma neoplasia do sistema nervoso simpático, e o segundo tumor sólido maligno extracraniano mais comum da infância. Na terapia antineoplásica, complicações orais podem ser observadas, dentre elas a mucosite oral (MO). Trata-se de uma inflamação aguda da mucosa, proveniente da toxicidade dos quimioterápicos. Este relato de caso enfatiza o manejo da MO, bem como sua influência na condição sistêmica e qualidade de vida. Paciente do sexo feminino, nove anos, apresentou recidiva de NB metastático, após tratamento de primeira linha. Admitida na unidade hospitalar para tratamento oncológico na enfermaria de pediatria oncológica, queixando-se de dor intensa em cavidade bucal e orofaringe, associada a pancitopenia severa febril. Ao exame físico apresentava disfagia e déficit ponderal grave, que debilitava a deglutição da própria saliva. O exame intraoral revelou lesões de MO grau 3, segundo a Organização Mundial de Saúde (OMS), em lábios, gengiva anterior e orofaringe. O tratamento consistiu em remoção de debris local, higiene da cavidade bucal com clorexidina 0,12% e utilizando haste flexível de algodão estéril tipo "Swab", visando controle microbiano local, diariamente. Além disso, foi aplicado no leito eritematoso e hemorrágico fotobiomodulação (660 nm, 50 mW, 2 J/cm2, 90 segundos) pontualmente nas áreas do leito da lesão e em varredura na região de orofaringe (sessões intercaladas). Foi prescrito acetato de racealfatocoferol (vitamina E) para ação antioxidante e hidratação dos lábios. Concomitante a mucosite, a paciente apresentou pancitopenia febril severa, sendo necessário uso de cefepima 150 mg/kg/dia, com coleta prévia de hemoculturas, fator estimulador de colônias de granulócitos, Fluconazol, hidratação e suporte nutricional. As hemoculturas foram negativas. As consequências da mucosite contribuíram para desnutrição e piora da qualidade de vida. Conclui-se que a intervenção odontológica em interdisciplinaridade, possibilitou o restabelecimento físico e emocional, possibilitando melhor uma qualidade de vida da paciente (AU).

Neuroblastoma (NB) is a neoplasia of the sympathetic nervous system and the second most common extracranial malignant solid tumor in childhood. In antineoplastic therapy, oral complications can be observed in antineoplastic therapy, among them oral mucositis (OM). It is an acute mucosa inflammation resulting from the toxicity of chemotherapy drugs. This case report emphasizes the management of OM and its influence on the systemic condition and quality of life. A female patient, nine years old, presented a recurrence of metastatic NB after first-line treatment. She was admitted to the hospital unit for oncological therapy in the pediatric oncology ward, complaining of severe pain in the oral cavity and oropharynx associated with severe febrile pancytopenia. On physical examination, she presented dysphagia and severe weight deficit, which weakened the swallowing of her saliva. According to the World Health Organization (WHO), the intraoral exam revealed third-grade OM lesions on the lips, anterior gum, and oropharynx. Treatment consisted of removing local debris, cleaning the oral cavity with 0.12% chlorhexidine, and using a flexible, sterile cotton swab, aiming for daily local microbial control. In addition, photobiomodulation (660 nm, 50 mW, 2 J/cm2, 90 seconds) was applied to the erythematous and hemorrhagic bed, punctually in the areas of the lesion and sweeps in the oropharynx region (interspersed sessions). Racealphatocopherol acetate (vitamin E) was prescribed for antioxidant action and lip hydration. Concomitant with mucositis, the patient had severe febrile pancytopenia, requiring cefepime 150 mg/kg/day, with previous collection of blood cultures, granulocyte colony-stimulating factor, fluconazole, hydration, and nutritional support. Blood cultures were negative. The consequences of mucositis contributed to malnutrition and worsened quality of life. It is concluded that the interdisciplinary dental intervention enabled physical and emotional restoration, enabling a better quality of life for the patient (AU).

Dinámica funcional de la comunidad bacteriana del lodo activado de una planta de tratamiento de agua residual y su relación con variables ambientales y de operación / Funcional dynamics of the bacterial community from activated sludge wastewater treatment plant and its relationship with environmental and operational variables

RESUMEN El proceso de lodos activados es comúnmente utilizado en plantas de tratamiento de aguas residuales (PTAR) para reducir la concentración de materia orgánica disuelta que llega en el afluente. A pesar de los avances en el estudio de las comunidades microbianas de los lodos activados, falta mucho para entender su potencial funcional y su variabilidad ante las fluctuaciones temporales del afluente y los cambios en la operación de las PTAR. En consecuencia, en este trabajo se analizó la variación del potencial metabólico de la comunidad bacteriana del lodo activado a lo largo de un ciclo anual y se relacionó esa dinámica con variables ambientales y operacionales de una PTAR con alcantarillado combinado. La predicción del metagenoma de la comunidad bacteriana se realizó con PICRUSt2. Esta aproximación permitió evidenciar el fenómeno de redundancia funcional en la comunidad. También, se logró analizar la fluctuación temporal de los genes asociados a procesos relacionados con los ciclos del nitrógeno y azufre y su relación con variables ambientales y operacionales de la PTAR. Se encontró que dichos procesos están relacionados diferencialmente con la precipitación y los cambios en la edad de lodo observados para el periodo estudiado. Estos resultados contribuyen al entendimiento de las dinámicas de la comunidad bacteriana con relación al funcionamiento de este tipo de sistemas biotecnológicos.

ABSTRACT Activated sludge process is commonly used in wastewater treatment plants (WWTP) where a microbial community removes the organic matter from the influent. Despite the advances in the study of this community, there is still a gap of knowledge about its functional potential and its variability due to temporary fluctuations of the influent and the WWTP operation. Therefore, this work analyzed the metabolic potential variation of the activated sludge bacterial community throughout an annual cycle. Furthermore, the dynamics of the bacterial community was related to environmental and operating variables of a WWTP with combined sewerage. The metagenome prediction of the bacterial community was carried out with PICRUSt2. This approach allowed to demonstrate the phenomenon of functional redundancy in the community. Moreover, the temporal fluctuation of genes associated with the nitrogen and sulfur cycles and their relationship with environmental and the operating variables of the WWTP were analyzed. It was found that those processes were differentially related to precipitation events and variations in the sludge age observed during the studied period. These results contribute to the understanding of the bacterial community dynamics in relation to the functioning of this type of biotechnological systems.

Set7 deletion attenuates isoproterenol-induced cardiac fibrosis and delays cardiac dysfunction.

Cardiovascular diseases are the main cause of death worldwide. Recent studies have revealed the influence of histone-modifying enzymes in cardiac remodeling and heart dysfunction. The Set7 methyltransferase regulates the expression of several genes through the methylation of histones and modulates the activity of non-histone proteins. However, the role of Set7 in cardiac remodeling and heart dysfunction remains unknown. To address this question, wild-type (WT) and Set7 knockout (KO) male mice were injected with isoproterenol or saline. WT mice injected with isoproterenol displayed a decrease in Set7 activity in the heart. In addition, WT and Set7 KO mice injected with isoproterenol exhibited cardiac hypertrophy. Interestingly, Set7 deletion exacerbated cardiac hypertrophy in response to isoproterenol but attenuated myocardial fibrosis. Echocardiograms revealed that WT mice injected with isoproterenol had lowered ejection fractions and fractional shortening, and increased E'-wave deceleration time and E/A ratio compared with their controls. Conversely, Set7 KO mice did not show alteration in these parameters in response to isoproterenol. However, prolonged exposure to isoproterenol induced cardiac dysfunction both in WT and Set7 KO mice. Both isoproterenol and Set7 deletion changed the transcriptional profile of the heart. Moreover, Set7 deletion increased the expression of Pgc1α and mitochondrial DNA content in the heart, and reduced the expression of cellular senescence and inflammation markers in response to isoproterenol. Taken together, our data suggest that Set7 deletion attenuates isoproterenol-induced myocardial fibrosis and delays heart dysfunction, suggesting that Set7 plays an important role in cardiac remodeling and dysfunction in response to stress.

Worldwide Systematic Review of GSTM1 and GSTT1 Null Genotypes by Continent, Ethnicity, and Therapeutic Area.

Glutathione S-transferase Mu 1 (GSTM1) and glutathione S-transferase theta 1 (GSTT1) enzymes are glutathione-S-transferases with broad significance for susceptibility or resistance to multifactorial human diseases, as well as detoxification of environmental chemicals and drugs. Moreover, some individuals may have a complete deletion of GSTM1 and GSTT1 genes, which can contribute to patient-to-patient variability in drug safety and efficacy. GSTM1 and GSTT1 gene deletion frequencies can vary according to ethnicity and continental origin of the studied population with implications for achieving the goal of precision/personalized medicine in clinical practice. We report here a worldwide systematic review of the null genotypes in these two clinically important genes by continents, ethnicities, and therapeutic areas (TAs). Searches were performed in the PubMed database covering the period from 1992 to 2020. Out of the 1925 articles included, most studies analyzed European individuals, corroborating the literature failure for not adequately considering the non-European ethnicities. The frequency of GSTM1 and GSTT1 null genotypes was higher in patients than in healthy volunteers. Conversely, in East Asians, higher frequencies of the null genotypes were observed in healthy volunteers than patients. Oncology was the most intensively studied TA (57% of the articles) in relation to GSTM1 and GSTT1. In all, these results demonstrate that there is an important gap in the literature in terms of failure to consider a broader range of populations, as well as diseases wherein GSTM1 and GSTT1 variations have clinical and biological implications. To achieve precision/personalized medicine on a global/worldwide scale, with equity and inclusiveness, this knowledge/research gap ought to be remedied in studies of GSTM1 and GSTT1 null genotypes. To the best of our knowledge, this is the largest systematic review conducted to date addressing the GSTM1 and GSTT1 null genotypes worldwide. The analyses from the 1925 articles highlighted the current knowledge gaps in different TAs, ethnicities, and populations. Filling these gaps is of importance, given the role these genes play in relation to the metabolism of substances to which we have frequent contact with, the associations observed between their deletion and diseases such as cancer, in addition to the interethnic differences observed for the deletion frequencies of these genes.

Set7 Deletion Prevents Glucose Intolerance and Improves the Recovery of Cardiac Function After Ischemia and Reperfusion in Obese Female Mice.

BACKGROUND/AIMS: An obesogenic diet (high fat and sugar, low fiber) is associated with an increased risk for metabolic and cardiovascular disorders. Previous studies have demonstrated that epigenetic changes can modify gene transcription and protein function, playing a key role in the development of several diseases. The methyltransferase Set7 methylates histone and non-histone proteins, influencing diverse biological and pathological processes. However, the functional role of Set7 in obesity-associated metabolic and cardiovascular complications is unknown. METHODS: Wild type and Set7 knockout female mice were fed a normal diet or an obesogenic diet for 12 weeks. Body weight gain and glucose tolerance were measured. The 3T3-L1 cells were used to determine the role of Set7 in white adipogenic differentiation. Cardiac morphology and function were evaluated by histology and echocardiography. An ex vivo Langendorff perfusion system was used to model cardiac ischemia/reperfusion (I/R). RESULTS: Here, we report that Set7 protein levels were enhanced in the heart and perigonadal adipose tissue (PAT) of female mice fed an obesogenic diet. Significantly, loss of Set7 prevented obesogenic diet-induced glucose intolerance in female mice although it did not affect the obesogenic diet-induced increase in body weight gain and adiposity in these animals, nor did Set7 inhibition change white adipogenic differentiation in vitro. In addition, loss of Set7 prevented the compromised cardiac functional recovery following ischemia and reperfusion (I/R) injury in obesogenic diet-fed female mice; however, deletion of Set7 did not influence obesogenic diet-induced cardiac hypertrophy nor the hemodynamic and echocardiographic parameters. CONCLUSION: These data indicate that Set7 plays a key role in obesogenic diet-induced glucose intolerance and compromised myocardial functional recovery after I/R in obese female mice.

Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p.

Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the early-stage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF-α, and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC.