Adapting the National Diabetes Prevention Program: Learning from Lifestyle Coach strategies and adjustments during the COVID-19 pandemic in the United States.

Background: The National Diabetes Prevention Program (National DPP) is an evidence-based lifestyle intervention successfully disseminated across the United States. Some adaptations have been made to address real-world needs, including during the COVID-19 pandemic. This study aims to qualitatively describe adaptations Lifestyle Coaches made in response to the pandemic. Methods: Between May and June 2021, Lifestyle Coaches (n = 300) from organizations across the United States answered open-ended survey questions about adjustments implemented during the pandemic. Survey responses were descriptively coded and codes were grouped into categories. Results: Nearly all coaches transitioned the format of their class from in-person to remote delivery (93.0 %; n = 279). Other commonly-reported strategies included adjusting contact with participants (48.0 %; n = 144), increasing support for participants (36.7 %; n = 110), and tailoring materials (28.3 %; n = 85). Conclusions: Maintaining these adaptations may address barriers to engagement in the National DPP and improve access to the program. Increased support for emotional symptoms and ensuring a patient-centered approach to care are particularly promising strategies.

Field study of an arsenic removal plant for drinking water using activated carbon and iron in a rural community in the province of Pisco, Peru.

The presence of arsenic in Peru is a serious public health problem due to the geographical extension of populations that consume water with arsenic concentrations above the value recommended by the World Health Organization (WHO). An arsenic removal plant has been studied in a community of 50 families located in the province of Pisco, Peru, a filter media of activated carbon impregnated with iron (AC-Fe) was applied, the adsorption capacity of the material was studied against As(V) and As(III) species, also, a possible decrease in the adsorption capacity of chloride and sulfate ions. Modifications were made to the plant layout based on filtration columns and workflows. The arsenic level was reduced to levels recommended by the WHO, the plant production was estimated at 9,000 volumes of water bed until reaching its breakpoint. An optimum working flow rate of 1.8 m3 h-1 was found, it was also found that the zeolite column used for suspended solids removal did not contribute to the reduction of arsenic concentration, and the presence of ions did not reduce the arsenic removal capacity.

Riesgo alto y muy alto de fracturas osteoporóticas en mujeres chilenas / High and very high risk of osteoporotic fractures in Chilean women

Objetivos: evaluar la aplicación de los umbrales de intervención basados en FRAX en mujeres chilenas. Recategorizar elriesgo de fractura osteoporótica para optimizar la selección de mujeres elegibles para intervención.Métodos: seleccionamos de la Encuesta Nacional de Salud (ENS) 2016-2017 (tercera versión) 1782 mujeres de 50 y másaños. Calculamos la probabilidad de fracturas osteoporóticas mayores y fracturas de cadera utilizando el modelo FRAXchileno. Calculamos la proporción de mujeres elegibles para tratamiento y evaluación de la densidad mineral ósea aplicandoumbrales de intervención específicos por edad de 50 a 90 años y un umbral híbrido que combina umbrales dependientes dela edad hasta los 75 años y, a partir de entonces, un umbral fijo con una única probabilidad de fractura hasta los 90 años.Resultados: veintidós mujeres (1,23 %) tenían una fractura previa y eran elegibles para tratamiento por este motivo.Utilizando umbrales específicos por edad, otras 33 mujeres fueron elegibles para tratamiento porque la probabilidad defractura osteoporótica mayor estaba por encima del umbral superior de evaluación. En 1107 (62,12 %) mujeres, se recomienda medir la densidad mineral ósea para poder recalcular el FRAX con la inclusión de la densidad mineral ósea del cuellofemoral. Con el umbral híbrido, otras 44 (3,69 %) mujeres fueron elegibles para el tratamiento y se recomendó la mediciónde la densidad ósea en 1169 mujeres (65,50 %). Si el tratamiento se asignaba solo con FRAX sin densidad mineral ósea,el número de mujeres elegibles para el tratamiento era de 70 (5,15 %) con un umbral de intervención específico para laedad y de 120 (6,72 %) con el umbral híbrido.Conclusiones: el umbral híbrido identifica más mujeres elegibles para tratamiento que los umbrales específicos poredad. La probabilidad promedio de fractura fue mayor con el umbral híbrido. Sobre esta base, nuestra posición es que serecomiende el umbral híbrido.(AU)

La fragmentación de la identidad en la disociación y en las psicosis / Identity fragmentation in dissociation and psychoses

En la psiquiatría actual tenemos mucho interés en dilucidar las semejanzas y las diferencias existentes entre las experiencias psicóticas y las experiencias disociativas, y en especial las dinámicas patogénicas que dan lugar a unas y a otras, y los abordajes terapéuticos más eficaces en cada caso. Desde el punto de vista de la identidad plural, estas dos experiencias constituyen las dos grandes crisis biográficas funcionales que dan lugar a la fragmentación de la identidad, con la pérdida de su unidad y continuidad, además de su autonomía y autoestima. Ambas se diferencian por los mecanismos de represión y disociación que están en el origen de las mismas. Ahora bien, cualquier enfoque o hipótesis con la que tratemos de entender estos dos tipos de experiencias no debe limitarse a comprenderlas en sus representaciones actuales y en las claves que hoy llamaríamos “científicas”, porque experiencias de este tipo han existido a lo largo de la historia de la humanidad reconceptualizadas de forma distinta, en otras claves culturales, espirituales, religiosas o morales. (AU)

In current psychiatry, there is a great interest in elucidating the existing similarities and differences between psychotic experiences and dissociative ones; especially, the pathogenic dynamics that give rise to both of them, as well as the most effective therapeutic approaches in each case. From the point of view of plural identity, these two experiences make up the two great functional biographic crises that give rise to identity fragmentation, with the loss of their unity and continuity, in addition to the loss of their autonomy and self-esteem. Both of them are differentiated by the repression and dissociation mechanisms that exist in their origin. That said, any approach or hypothesis that we may use to try to understand these two types of experiences should not be limited to understanding them in their current representations and with the keys that we currently call “scientific”, because experiences of this type have existed throughout the history of humanity, reconceptualized differently in other cultural, spiritual, religious or moral keys. (AU)

Couple-based lifestyle intervention to prevent type 2 diabetes: protocol for a randomised pilot trial.

INTRODUCTION: Type 2 diabetes is prevalent among US adults. Lifestyle interventions that modify health behaviours prevent or delay progression to diabetes among individuals at high risk. Despite the well-documented influence of individuals' social context on their health, evidence-based type 2 diabetes prevention interventions do not systematically incorporate participants' romantic partners. Involving partners of individuals at high risk for type 2 diabetes in primary prevention may improve engagement and outcomes of programmes. The randomised pilot trial protocol described in this manuscript will evaluate a couple-based lifestyle intervention to prevent type 2 diabetes. The objective of the trial is to describe the feasibility of the couple-based intervention and the study protocol to guide planning of a definitive randomised clinical trial (RCT). METHODS AND ANALYSIS: We used community-based participatory research principles to adapt an individual diabetes prevention curriculum for delivery to couples. This parallel two-arm pilot study will include 12 romantic couples in which at least one partner (ie, 'target individual') is at risk for type 2 diabetes. Couples will be randomised to either the 2021 version of the CDC's PreventT2 curriculum designed for delivery to individuals (six couples), or PreventT2 Together, the adapted couple-based curriculum (six couples). Participants and interventionists will be unblinded, but research nurses collecting data will be blinded to treatment allocation. Feasibility of the couple-based intervention and the study protocol will be assessed using both quantitative and qualitative measures. ETHICS AND DISSEMINATION: This study has been approved by the University of Utah IRB (#143079). Findings will be shared with researchers through publications and presentations. We will collaborate with community partners to determine the optimal strategy for communicating findings to community members. Results will inform a subsequent definitive RCT. TRIAL REGISTRATION NUMBER: NCT05695170.

Advanced Cell Therapy: Beyond the last Frontier in the Treatment of Cancer. A Historical Perspective Emphasizing the Work of Nobel Prize Laureates.

During the last five decades different therapies have been developed for the treatment of cancer, and as a result, patients can now live longer and better lives. Among such therapies, hematopoietic cell transplantation and immunotherapy have played key roles. In this short article, we present our particular point of view on the development of these two cellular therapies. We have focused on a historical perspective emphasizing the work of some of the Nobel Prize winners whose studies constituted cornerstones in our knowledge of the biology of cancer and in our fight against this devastating disease.

Decreasing incidence rates of osteoporotic hip fractures in Ecuador during the COVID-19 pandemic.

We aimed at comparing the incidence of hip fractures in older adults from Ecuador before and during the COVID-19 pandemic. There was a significant reduction in the number of hip fractures, with no change in the length of hospital stay, mortality, and case-fatality rate, during the period of social isolation. INTRODUCTION: The impact that the COVID-19 pandemic has had on fragility fractures is being recently evaluated in the literature. Despite this, data from Latin America in this regard is scarce. PURPOSE: This study aims to compare the incidence rate of hip fractures before and during the COVID-19 pandemic in older adults who received care in the public and private health system of Ecuador. METHODS: This was a descriptive and retrospective study that analyzed data of individuals aged 60 years and older who had hip fractures before and during the COVID-19 pandemic. The information was obtained from the National Hospital Discharge Yearbook. We calculated the incidence, average length of hospital stay, mortality, and case-fatality rate associated with hip fractures. RESULTS: There was a significant reduction in the incidence of hip fractures in adults 60 or older during the period of social isolation due to COVID-19. Between March and December 2019, there was an incidence of 152 hip fractures per 100,000 inhabitants, whereas during the same period but in 2020 in the incidence was 110 per 100,000 inhabitants (p < 0.0001). The main decrease was observed in women aged 80 or more. The average length of hospital stay did not show significant changes. Mortality displayed a non-significant decrease (p = 0.14), although this decrease was significant among women (p = 0.02). Case-fatality rate showed a non-significant increase for the whole group (p = 0.68) and for men (p = 0.09). CONCLUSION: Hip fracture rates decreased significantly in adults aged 60 and older in 2020 compared to 2019. This decrease of hip fracture incidence rates was mainly due to the reductions observed in older people and women. The average length of hospital stay, mortality, and case-fatality rate associated with hip fractures did not show significant changes during the pandemic.

Applying best practices for health disparities work to create a treatment adaptation framework for culturally diverse communities: A mixed-methods approach.

OBJECTIVE: Integrating best practices for health disparities to adapt evidence-based treatments is imperative to adequately meet the needs of diverse cultures, particularly ones that therapists can apply flexibility across multiple diverse communities. METHOD: Using a mixed-methods, community-engaged approach, we examined how a range of community participants (N = 169) defined mental health, perceived barriers to treatment, and used culturally based coping methods to manage their mental health. Phase 1 (n = 49) included qualitative focus group data from five distinct racial/ethnic communities (African immigrants/refugees, Black/African Americans, Hispanics, Pacific Islanders, and American Indians). Phase 2 included quantitative surveys from members of four of these communities (n = 59) and the frontline providers serving them (n = 61). RESULTS: The communities and providers highlighted chronic worry and distress related to daily activities as primary treatment concerns. Further, this mixed-methods data informed our proposed best practice treatment adaptation framework using chronic worry as an example. CONCLUSION: The main aims of this study were to exemplify best practices for addressing mental health inequities in communities of color in terms of (a) conducting health disparities research and (b) applying a treatment adaptation framework for culturally responsive clinical care. Specific features of how this framework was conceived and applied provide a unique and critical view into integrating best practices to address health disparities in diverse communities. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Developing an equitable intervention approach for communities of color: mental health and co-occurring physical health concerns in the context of the COVID-19 pandemic.

The COVID-19 pandemic has exacerbated disparities in mental health treatment for people of color in the USA. Meeting the needs of those most burdened by this disparity will require swift and tactical action in partnership with these communities. The purpose of this paper is to describe how a community-based participatory research approach was employed to assess the priorities and needs of four communities of color (African immigrant, Hispanic/Latino, Black/African American, and Pacific Islander) in a major U.S. city. A brief quantitative survey devised jointly by community leaders and the research team was deployed to community members (N = 59) in the fall of 2020. The most endorsed mental health issues across the communities were excessive worry (51%) and stress regarding COVID-19, racism, and immigration policies (49%). The most endorsed physical health concerns included sleep difficulties (44%), headaches, and backaches (each 39%). Physical symptoms predicted the endorsement of a mental health issue above and beyond COVID-19-related hardships, multiplying the odds of reporting an issue by 1.73 per physical health concern endorsed. Based on these findings, the community-research team conceptualized and proposed an evidence-based, effectiveness-implementation hybrid type-2 intervention approach for chronic worry and daily stress. This paper highlights detail on how the community-research team arrived at the proposed multilevel intervention that addresses community-stated barriers to mental health treatment (e.g., preferring trusted health workers to deliver emotional health treatments) and considers the burden of the additional stressful context of COVID-19.

Diverse community members and university researchers collaborated on the development of an equitable intervention approach for community members' mental health needs.

I'm Lovin' It: How Fast Food Advertising Influences Meat-Eating Preferences.

Overconsumption of red and processed meat is associated with a multitude of negative health outcomes. Previous research shows exposure to advertising messaging can influence dietary behaviors but research on the influence of meat advertising on diet, specifically, is scant. Theoretically informed by the Reasoned Action Approach, the present experiment randomly assigned participants to view a version of a print McDonald's advertisement that included meat imagery (a Big Mac), non-meat imagery (French fries), or no food (just the McDonalds' logo and slogan), which acted as a control. An online survey in the United States included 514 U.S. adults (Mage = 51 years). Participants exposed to meat imagery compared to the non-meat imagery reported a higher desire to eat meat. The meat imagery and control conditions were also significantly associated with increased cognitive accessibility of meat concepts, compared to when respondents were shown the no-meat condition. Desire to eat meat, but not the cognitive accessibility of meat concepts, was significantly associated with attitude, normative pressure, and perceived behavioral control for avoiding eating meat one day per week; these constructs predicted intention and willingness to avoid meat. Results indicate that exposure to meat imagery in advertising does have the potential to influence meat consumption behavior and also has implications for the use of meat imagery in persuasive messaging for public health campaigns.

Barriers to participation and lifestyle change among lower versus higher income participants in the National Diabetes Prevention Program: lifestyle coach perspectives.

Individuals from socioeconomically disadvantaged groups have lesser participation and success in the National Diabetes Prevention Program (NDPP). Barriers to NDPP participation and lifestyle change were examined from the perspective of Lifestyle Coaches serving lower versus higher income participants. Lifestyle Coaches (n = 211) who serve lower income (n = 82) or higher income (n = 129) participants reported on observed barriers to NDPP participation and lifestyle change and ranked the three most significant barriers to (a) NDPP participation and (b) lifestyle change. Group differences in number/type of barriers were examined using t-tests and chi-square analyses, and ranking differences were examined using multilevel cumulative logit models. Lifestyle Coaches of lower income (versus higher income) participants reported two additional barriers on average. Ranked barriers to participation were similar between groups, and notably included physical/emotional barriers. However, for lifestyle change, those serving lower income groups were more likely to rank lack of access to healthy grocery stores, but less likely to rank low motivation and lack of family support. Lifestyle Coaches of lower income participants were less likely to rank long wait period prior to enrollment as the most significant barrier to participation, and to rank lack of time off from work as the most significant barrier to lifestyle change. Despite more barriers observed among lower versus higher income participants, overlap in the most significant barriers highlights the potential utility of widely addressing common barriers among NDPP participants. In particular, physical and emotional barriers have been overlooked, yet deserve greater attention in future research and practice.

The National Diabetes Prevention Program (NDPP) has less successfully reached and changed the lifestyles of lower income (versus higher income) adults in the USA who are at high risk for type 2 diabetes. In a nationwide online survey, we asked Lifestyle Coaches who deliver the NDPP to identify up to 37 potential barriers to participation and success that they had observed among their participants. We then compared the number, type, and rankings of the most significant barriers to participation and success in the NDPP from the perspective of Lifestyle Coaches estimating the majority of their participants had lower versus higher incomes. Lifestyle Coaches delivering the NDPP to lower income participants reported an average of two additional barriers to participation and success than those delivering the program to higher income participants. The barriers ranked among the most significant to NDPP participation and lifestyle change were generally similar among Lifestyle Coaches working with lower versus higher income participants. Top-ranked barriers included physical/emotional symptoms (e.g., anxiety, depression) as well as barriers previously reported in studies focused on NDPP participants. It is critical that barriers be carefully evaluated and addressed to improve the nationwide impact of the NDPP.

Antifungal activity screening of fractions from Annona cherimola Mill. leaf extract against Fusarium oxysporum.

The antifungal effect of ethanolic extract fractions of Annona cherimola leaves against the mycelial growth of Fusarium oxysporum was studied. The ethanolic crude extract was solvent partitioned and the ethyl acetate phase was fractionated by column or preparative thin-layer chromatography. All fractions were developed on TLC and analyzed for acetogenins (ACG) with Kedde reagent. The antifungal effect assays were carried out in vitro by the diffusion method on PDA plates. The ethanolic extract of A. cherimola leaves was highly active against F. oxysporum growth; subfractions obtained from the antifungal screening had a significant effect (p < 0.05) on the F. oxysporum growth parameters. The screening showed that as the purification steps progressed, the inhibition of mycelial growth increased. Six bioactive ACG (Annomolon-B, 34-epi annomolon B, almunequin, cherimoline 1, cherimoline 2, and isocherimoline 1) were identified by LC-QTOF-MS/MS. These findings suggested that bioactive ACG from A. cherimola leaves could be an alternative resource of a promising botanical fungicide to control plant diseases caused by F. oxysporum.

Promises and Pitfalls of Dyads in the National Diabetes Prevention Program: Lifestyle Coach Perspectives.

PURPOSE: To describe Lifestyle Coach perceptions of dyads (i.e., family members and/or friends) in the National Diabetes Prevention Program (NDPP). DESIGN: Qualitative evaluation of cross-sectional survey responses. SETTING: Online. PARTICIPANTS: Lifestyle Coaches (n=253) with experience teaching at least one in-person year-long NDPP cohort at a CDC-recognized organization. MEASURES: Survey included items on background and experience with dyadic approach, as well as open-ended items on the benefits and challenges observed when working with dyads in the NDPP. ANALYSIS: Lifestyle Coach background and experience were analyzed descriptively in SPSS. Open-ended responses were content coded in ATLAS.ti using qualitative description, and then grouped into categories. RESULTS: Most Lifestyle Coaches (n=210; 83.0%) reported experience delivering the NDPP to dyads. Benefits of a dyadic approach included having a partner in lifestyle change, superior outcomes and increased engagement, and positive "ripple effects." Challenges included difficult relationship dynamics, differences between dyad members, negative "ripple effects," and logistics. CONCLUSION: Lifestyle Coaches described a number of benefits, as well as some challenges, with a dyadic approach to the NDPP. Given the concordance between close others in lifestyle and other risk factors for type 2 diabetes, utilizing a dyadic approach in the NDPP has the potential to increase engagement, improve outcomes, and extend the reach of the program.

Family and Cultural Perceptions About Meat Consumption among Hispanic/Latino and White Adults in the United States.

The study examines the extent to which Hispanic/Latino Americans and non-Hispanic White Americans report that meat is an important aspect of their cultural beliefs and practices. An online national survey was completed using quota sampling to recruit approximately equal groups of U.S. Hispanic/Latino and non-Hispanic White Americans (n = 512). A path model predicting willingness to reduce meat consumption was estimated. Hispanic/Latino Americans viewed meat as a more integral aspect of their cultural food practices, and reported that meat dishes comprise a higher percentage of their culture's traditional foods. Cultural beliefs were associated with meat consumption and willingness to reduce meat consumption.

From reaction kinetics to dementia: A simple dimer model of Alzheimer's disease etiology.

Oligomers of the amyloid ß-protein (Aß) have been implicated in the pathogenesis of Alzheimer's disease (AD) through their toxicity towards neurons. Understanding the process of oligomerization may contribute to the development of therapeutic agents, but this has been difficult due to the complexity of oligomerization and the metastability of the oligomers thus formed. To understand the kinetics of oligomer formation, and how that relates to the progression of AD, we developed models of the oligomerization process. Here, we use experimental data from cell viability assays and proxies for rate constants involved in monomer-dimer-trimer kinetics to develop a simple mathematical model linking Aß assembly to oligomer-induced neuronal degeneration. This model recapitulates the rapid growth of disease incidence with age. It does so through incorporation of age-dependent changes in rates of Aß monomer production and elimination. The model also describes clinical progression in genetic forms of AD (e.g., Down's syndrome), changes in hippocampal volume, AD risk after traumatic brain injury, and spatial spreading of the disease due to foci in which Aß production is elevated. Continued incorporation of clinical and basic science data into the current model will make it an increasingly relevant model system for doing theoretical calculations that are not feasible in biological systems. In addition, terms in the model that have particularly large effects are likely to be especially useful therapeutic targets.

Modelos matemáticos aplicados a la Epidemiología / Mathematical models applied to Epidemiology / Modelos matemáticos aplicados à Epidemiologia

RESUMEN Un modelo matemático es una descripción matemática (a menudo por medio de una función o una ecuación) de un fenómeno del mundo real, como el tamaño de una población, la expectativa de vida de una persona al nacer o la propagación de una epidemia. Para ver la importancia de estos en las Ciencias de la Salud, específicamente en la especialidad de Higiene y Epidemiología mostramos dos de ellos para predecir el comportamiento de epidemias. El primero lo exponemos mediante una ecuación diferencial de 1er orden y el segundo mediante un sistema de ecuaciones diferenciales.

ABSTRACT A mathematical model is a mathematical description (often by means of a function or an equation) of a real-world phenomenon, such as the size of a population, the life expectancy of a person at birth, or the spread of an epidemic. To see the importance of these in Health Sciences, specifically in the specialty of Hygiene and Epidemiology, we show two of them to predict the behavior of epidemics. We expose the first through a 1st order differential equation and the second through a system of differential equations.

RESUMO Um modelo matemático é uma descrição matemática (frequentemente por meio de uma função ouequação) de um fenômeno do mundo real, como o tamanho de uma população, a expectativa de vida de uma pessoaao nascer ou a propagação de uma epidemia. Para perceber a importância destesnas Ciências da Saúde, especificamente na especialidade Higiene e Epidemiologia, mostramos dois deles para prever o comportamento de epidemias. Expomos o primeiro por meio de uma equação diferencial de 1ª ordem e o segundo por meio de um sistema de equaçõ es diferenciais.

Modelos matemáticos aplicados a la Epidemiología / Mathematical models applied to Epidemiology

Un modelo matemático es una descripción matemática (a menudo por medio de una función o una ecuación) de un fenómeno del mundo real, como el tamaño de una población, la expectativa de vida de una persona al nacer o la propagación de una epidemia. Para ver la importancia de estos en las Ciencias de la Salud, específicamente en la especialidad de Higiene y Epidemiología mostramos dos de ellos para predecir el comportamiento de epidemias. El primero lo exponemos mediante una ecuación diferencial de 1er orden y el segundo mediante un sistema de ecuaciones diferenciales(AU)

A mathematical model is a mathematical description (often by means of a function or an equation) of a real-world phenomenon, such as the size of a population, the life expectancy of a person at birth, or the spread of an epidemic. To see the importance of these in Health Sciences, specifically in the specialty of Hygiene and Epidemiology, we show two of them to predict the behavior of epidemics. We expose the first through a 1st order differential equation and the second through a system of differential equations(EU)

The Mediating Role of Family and Cultural Food Beliefs on the Relationship between Family Communication Patterns and Diet and Health Issues across Racial/Ethnic Groups.

Consumption of red meat has been linked to a variety of health issues, yet Americans are resistant to reducing their meat consumption. Family communication environments shape beliefs about food and meat consumption, and therefore are locations for potential interventions to change the way people think about food. Families are embedded in cultures, and both family and cultural norms shape beliefs about what people should eat. This study (N = 773) is interested in understanding how family communication is associated with food beliefs, meat consumption, and health issues across three racial/ethnic groups: Black/African American (n = 256), Hispanic (n = 260), non-Hispanic White (n = 257). Structural equation modeling results showed that conversation orientation was consistently associated with stronger endorsement of family cultural food beliefs across race/ethnicity groups. Family food beliefs were associated with either more health issues or more meat consumption depending on race/ethnicity and mediated the association between conversation orientation and health issues/meat consumption. Conversation orientation moderated the association between conformity orientation and food beliefs for Hispanic and non-Hispanic White participants. Implications for family communication patterns theory and health scholars are discussed along with recommendations for culturally tailored family-focused health interventions.

Osmo- and cryotherapy of sugarcane (Saccharum spp. L.) shoot-tips infected with sugarcane mosaic virus (SCMV).

In this work, we investigated the effect of different osmoprotective treatments and of cryopreservation using a droplet-vitrification (D-V) protocol to eliminate sugarcane mosaic virus (SCMV) of shoot-tips excised from in vitro propagated infected plantlets. Shoot-tips of sugarcane (Saccharum spp. L.) were precultured on semisolid MS medium supplemented with 0.3 M sucrose for 1 day, loaded in solution with 0.4 M sucrose and 2 M glycerol for 30 min and exposed to plant vitrification solution 2 for 15 min at room temperature prior to ultra-rapid cooling in liquid nitrogen. Virus indexing was performed by the DAS-ELISA immunoenzymatic test. The presence of SCMV was confirmed in the donor-plantlets derived of infected field material. No virus was detected in the regenerated plantlets from shoot-tips subjected to cryopreservation protocol. The progressive decrease in absorbances occurred from the first preculture treatment and no significant differences (P ≤ 0.05) were found with respect to following steps of D-V protocol. These results indicate that the osmotic dehydration treatments (osmotherapy) and cryopreservation (cryotherapy) may be potentially effective strategies to remove the SCMV from infected plants.