Comparative evaluation of coronal discoloration induced by two triple antibiotic revascularization protocols when used at varying depths of temporary sealing material at the end of varying time periods.

AIM: The aim of this study is to evaluate and compare the coronal discoloration induced by two Triple antibiotic paste (TAP 1 and 2), when used with varying depths of 2 and 4 mm of temporary sealing materials at the end of 0, 3, and 6 weeks. OBJECTIVES: (1) To evaluate coronal discoloration induced by TAP-1 when the depth of the temporary restorative material is 2 mm below cementoenamel junction at the end of 0, 3, and 6 weeks. MATERIALS AND METHODS: Forty extracted permanent maxillary central and lateral incisors or single-rooted tooth were collected and chosen for the study. They were then divided into two groups and each group was then subdivided into two subgroups. RESULTS: Both the triple antibiotic paste showed discoloration but it reduced with increase in thickness of temporary cement. CONCLUSION: TAP 1 and TAP 2 both showed an increase in discoloration, greater discoloration was seen with TAP, containing minocycline. There was a steady change in the discoloration from 0 to 6th week. In both the groups, TAP 1 and TAP 2, there was a reduction in discoloration with increase thickness of the temporary restorative material.

Morphokinetic analysis of cleavage stage embryos and its relationship to aneuploidy in a retrospective time-lapse imaging study.

PURPOSE: To analyze differences in morphokinetic parameters of chromosomally normal and aneuploid embryos utilizing time-lapse imaging and CGH microarray analysis. METHODS: This retrospective cohort study included patients undergoing IVF treatment and preimplantation genetic diagnosis for sex selection. A total of 460 embryos cultured in incubators with time-lapse imaging system (EmbryoScope) were selected for biopsy on day 3 of development. Subsequently, CGH microarray analysis was performed for aneuploidy screening of 24 chromosomes. Kinetic parameters including time for appearance of second polar body (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPBf), time to division to 2(t2), 3(t3), 4(t4), 5(t5) cells, length of second and third cell cycle (CC2= t3 t2, CC3=t5-t3), synchrony of cell division from 2 to 4 cells (S2=t4-t3) and interval t5-t2 were analyzed to compare chromosomally normal and abnormal embryos. RESULTS: The mean time durations for tPNf, t2, t5, CC2, CC3, t5-t2 differed significantly between normal and abnormal embryos. CONCLUSIONS: Time-lapse imaging morphokinetics may play a role in early prediction of aneuploid embryos due to differences in kinetic behavior that may aid in improving clinical outcome.