[DNA content in dysplasias and early invasive forms of cervical cancer]. / Soderzhanie DNK pri dispalziiakh i rannikh invazivnykh formakh raka sheiki matki.

Cytological preparations of normal cervical epithelium and in different forms of the epithelial dysplasia and in early cervical cancer were studied in 8 healthy females and in 35 patients. To determine the content of DNA in these cells a fluorescent variant of the Feulgen reaction was employed, using the national drug rivanol as fluorochromium in the Shiff reagent. The amount of DNA in cell nuclei of the normal epithelium and in moderate dysplasia is shown to correspond to diploid, while in marked dysplasia the content of DNA varies from di-to tetraploid values. But there are some cases when in marked dysplasia the DNA content in cells is diploid, i. e. it is characteristic of moderate dysplasia, or considerably exceeds tetraploid level, and it is in such cases that histological assays reveal early forms of cancer. Invasive cancer is charcterized by a sharp increase in the DNA content in most cells and by a considerable number of polyploid cells. Fluorescence-microscopy may serve as a valuable diagnostic adjunct to recognize early forms of cervical cancer and be used clinically.

[Clinical evaluation of the dysplasias and the early forms of cervical cancer]. / Klinicheskaia otsenka displazii i rannie formy raka sheiki matki.

One hundred and forty patients, admitted to the Center of Oncology Research of the USSR Academy of Medical Sciences with a suspicion to cervical cancer, were examined by the complex method comprising in addition to routine gynecological examination also extended colposcopy, cytological investigation of smears from pathological foci surface as well as histological studies, "aimed biopsy and cervical canal curetting. It was found that the cytological picture of moderate dysplasia of cervical epithelium is more frequently recognized in patients with pseuderosions developed with unchanged in size and form uterine cervix in the background, in the presence of benign colposcopic picture. The cytological picture of marked epithelial dysplasia and dysplasia with clear-cut cell atypism is observed in patients with bleeding pseuderosions against the background of hypertrophic and deformed cervix. Histological assay in these patients revealed early cervical cancer in 67% of observations. Marked extensive leucoplakia of the cervix found in clinical examination is manifested by different forms of epithelial dysplasia, while histological assay revealed early cervical cancer in 29.4% of observations.