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1.
Int J Mol Sci ; 24(3)2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36768148

RESUMO

Chronic nasal carriage of Staphylococcus aureus (SA) has been shown to be significantly higher in GPA patients when compared to healthy subjects, as well as being associated with increased endonasal activity and disease relapse. The aim of this study was to investigate SA involvement in GPA by applying a network-based analysis (NBA) approach to publicly available nasal transcriptomic data. Using these data, our NBA pipeline generated a proteinase 3 (PR3) positive ANCA associated vasculitis (AAV) disease network integrating differentially expressed genes, dysregulated transcription factors (TFs), disease-specific genes derived from GWAS studies, drug-target and protein-protein interactions. The PR3+ AAV disease network captured genes previously reported to be dysregulated in AAV associated. A subnetwork focussing on interactions between SA virulence factors and enriched biological processes revealed potential mechanisms for SA's involvement in PR3+ AAV. Immunosuppressant treatment reduced differential expression and absolute TF activities in this subnetwork for patients with inactive nasal disease but not active nasal disease symptoms at the time of sampling. The disease network generated identified the key molecular signatures and highlighted the associated biological processes in PR3+ AAV and revealed potential mechanisms for SA to affect these processes.


Assuntos
Vasculite Associada a Anticorpo Anticitoplasma de Neutrófilos , Granulomatose com Poliangiite , Staphylococcus aureus Resistente à Meticilina , Doenças Nasais , Infecções Estafilocócicas , Humanos , Granulomatose com Poliangiite/genética , Granulomatose com Poliangiite/diagnóstico , Staphylococcus aureus/genética , Anticorpos Anticitoplasma de Neutrófilos , Vasculite Associada a Anticorpo Anticitoplasma de Neutrófilos/diagnóstico , Mieloblastina
3.
Clin Pharmacol Ther ; 109(3): 605-618, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-32686076

RESUMO

Drug development in oncology commonly exploits the tools of molecular biology to gain therapeutic benefit through reprograming of cellular responses. In immuno-oncology (IO) the aim is to direct the patient's own immune system to fight cancer. After remarkable successes of antibodies targeting PD1/PD-L1 and CTLA4 receptors in targeted patient populations, the focus of further development has shifted toward combination therapies. However, the current drug-development approach of exploiting a vast number of possible combination targets and dosing regimens has proven to be challenging and is arguably inefficient. In particular, the unprecedented number of clinical trials testing different combinations may no longer be sustainable by the population of available patients. Further development in IO requires a step change in selection and validation of candidate therapies to decrease development attrition rate and limit the number of clinical trials. Quantitative systems pharmacology (QSP) proposes to tackle this challenge through mechanistic modeling and simulation. Compounds' pharmacokinetics, target binding, and mechanisms of action as well as existing knowledge on the underlying tumor and immune system biology are described by quantitative, dynamic models aiming to predict clinical results for novel combinations. Here, we review the current QSP approaches, the legacy of mathematical models available to quantitative clinical pharmacologists describing interaction between tumor and immune system, and the recent development of IO QSP platform models. We argue that QSP and virtual patients can be integrated as a new tool in existing IO drug development approaches to increase the efficiency and effectiveness of the search for novel combination therapies.


Assuntos
Alergia e Imunologia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Desenvolvimento de Medicamentos , Inibidores de Checkpoint Imunológico/uso terapêutico , Oncologia , Simulação de Dinâmica Molecular , Neoplasias/tratamento farmacológico , Biologia de Sistemas , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Protocolos de Quimioterapia Combinada Antineoplásica/farmacocinética , Simulação por Computador , Humanos , Inibidores de Checkpoint Imunológico/efeitos adversos , Inibidores de Checkpoint Imunológico/farmacocinética , Modelos Imunológicos , Terapia de Alvo Molecular , Neoplasias/imunologia , Neoplasias/metabolismo , Microambiente Tumoral
4.
Clin Pharmacol Ther ; 107(4): 858-870, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31955413

RESUMO

Application of contemporary molecular biology techniques to clinical samples in oncology resulted in the accumulation of unprecedented experimental data. These "omics" data are mined for discovery of therapeutic target combinations and diagnostic biomarkers. It is less appreciated that omics resources could also revolutionize development of the mechanistic models informing clinical pharmacology quantitative decisions about dose amount, timing, and sequence. We discuss the integration of omics data to inform mechanistic models supporting drug development in immuno-oncology. To illustrate our arguments, we present a minimal clinical model of the Cancer Immunity Cycle (CIC), calibrated for non-small cell lung carcinoma using tumor microenvironment composition inferred from transcriptomics of clinical samples. We review omics data resources, which can be integrated to parameterize mechanistic models of the CIC. We propose that virtual trial simulations with clinical Quantitative Systems Pharmacology platforms informed by omics data will be making increasing impact in the development of cancer immunotherapies.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Coleta de Dados/métodos , Imunoterapia/métodos , Neoplasias Pulmonares/terapia , Oncologia/métodos , Farmacologia Clínica/métodos , Carcinoma Pulmonar de Células não Pequenas/imunologia , Coleta de Dados/estatística & dados numéricos , Desenvolvimento de Medicamentos/métodos , Desenvolvimento de Medicamentos/estatística & dados numéricos , Humanos , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/imunologia , Imunoterapia/estatística & dados numéricos , Neoplasias Pulmonares/imunologia , Oncologia/estatística & dados numéricos , Farmacologia Clínica/estatística & dados numéricos
6.
CPT Pharmacometrics Syst Pharmacol ; 8(5): 259-272, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30667172

RESUMO

The lack of standardization in the way that quantitative and systems pharmacology (QSP) models are developed, tested, and documented hinders their reproducibility, reusability, and expansion or reduction to alternative contexts. This in turn undermines the potential impact of QSP in academic, industrial, and regulatory frameworks. This article presents a minimum set of recommendations from the UK Quantitative and Systems Pharmacology Network (UK QSP Network) to guide QSP practitioners seeking to maximize their impact, and stakeholders considering the use of QSP models in their environment.


Assuntos
Hormônio Paratireóideo/farmacologia , Biologia de Sistemas/normas , Humanos , Modelos Biológicos , Hormônio Paratireóideo/efeitos adversos , Guias de Prática Clínica como Assunto , Reprodutibilidade dos Testes , Reino Unido
7.
Brief Bioinform ; 20(2): 540-550, 2019 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-30462164

RESUMO

Life science researchers use computational models to articulate and test hypotheses about the behavior of biological systems. Semantic annotation is a critical component for enhancing the interoperability and reusability of such models as well as for the integration of the data needed for model parameterization and validation. Encoded as machine-readable links to knowledge resource terms, semantic annotations describe the computational or biological meaning of what models and data represent. These annotations help researchers find and repurpose models, accelerate model composition and enable knowledge integration across model repositories and experimental data stores. However, realizing the potential benefits of semantic annotation requires the development of model annotation standards that adhere to a community-based annotation protocol. Without such standards, tool developers must account for a variety of annotation formats and approaches, a situation that can become prohibitively cumbersome and which can defeat the purpose of linking model elements to controlled knowledge resource terms. Currently, no consensus protocol for semantic annotation exists among the larger biological modeling community. Here, we report on the landscape of current annotation practices among the COmputational Modeling in BIology NEtwork community and provide a set of recommendations for building a consensus approach to semantic annotation.


Assuntos
Disciplinas das Ciências Biológicas , Biologia Computacional/métodos , Simulação por Computador , Bases de Dados Factuais , Semântica , Humanos , Software
8.
CPT Pharmacometrics Syst Pharmacol ; 8(2): 77-86, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30358157

RESUMO

Parkinsons disease (PD) is a progressive neurodegenerative disease with substantial and growing socio-economic burden. In this multifactorial disease, aging, environmental, and genetic factors contribute to neurodegeneration and dopamine (DA) deficiency in the brain. Treatments aimed at DA restoration provide symptomatic relief, however, no disease modifying treatments are available, and PD remains incurable to date. Mathematical modeling can help understand such complex multifactorial neurological diseases. We review mathematical modeling efforts in PD with a focus on mechanistic models of pathogenic processes. We consider models of α-synuclein (Asyn) aggregation, feedbacks among Asyn, DA, and mitochondria and proteolytic systems, as well as pathology propagation through the brain. We hope that critical understanding of existing literature will pave the way to the development of quantitative systems pharmacology models to aid PD drug discovery and development.


Assuntos
Modelos Biológicos , Doença de Parkinson/metabolismo , Encéfalo/metabolismo , Dopamina/metabolismo , Humanos , Mitocôndrias/metabolismo , Doença de Parkinson/patologia , alfa-Sinucleína/metabolismo
9.
Nucleic Acids Res ; 46(D1): D1248-D1253, 2018 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-29106614

RESUMO

BioModels serves as a central repository of mathematical models representing biological processes. It offers a platform to make mathematical models easily shareable across the systems modelling community, thereby supporting model reuse. To facilitate hosting a broader range of model formats derived from diverse modelling approaches and tools, a new infrastructure for BioModels has been developed that is available at http://www.ebi.ac.uk/biomodels. This new system allows submitting and sharing of a wide range of models with improved support for formats other than SBML. It also offers a version-control backed environment in which authors and curators can work collaboratively to curate models. This article summarises the features available in the current system and discusses the potential benefit they offer to the users over the previous system. In summary, the new portal broadens the scope of models accepted in BioModels and supports collaborative model curation which is crucial for model reproducibility and sharing.


Assuntos
Curadoria de Dados , Modelos Biológicos , Software , Coleta de Dados , Curadoria de Dados/métodos , Internet , Interface Usuário-Computador
10.
IEEE Trans Biomed Eng ; 63(10): 2007-14, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27305665

RESUMO

OBJECTIVE: Whole-cell (WC) modeling is a promising tool for biological research, bioengineering, and medicine. However, substantial work remains to create accurate comprehensive models of complex cells. METHODS: We organized the 2015 Whole-Cell Modeling Summer School to teach WC modeling and evaluate the need for new WC modeling standards and software by recoding a recently published WC model in the Systems Biology Markup Language. RESULTS: Our analysis revealed several challenges to representing WC models using the current standards. CONCLUSION: We, therefore, propose several new WC modeling standards, software, and databases. SIGNIFICANCE: We anticipate that these new standards and software will enable more comprehensive models.


Assuntos
Simulação por Computador , Modelos Biológicos , Software , Biologia de Sistemas/normas , Biologia Computacional , Técnicas Citológicas , Feminino , Humanos , Masculino , Biologia de Sistemas/educação , Biologia de Sistemas/organização & administração
11.
Nucleic Acids Res ; 43(Database issue): D542-8, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25414348

RESUMO

BioModels (http://www.ebi.ac.uk/biomodels/) is a repository of mathematical models of biological processes. A large set of models is curated to verify both correspondence to the biological process that the model seeks to represent, and reproducibility of the simulation results as described in the corresponding peer-reviewed publication. Many models submitted to the database are annotated, cross-referencing its components to external resources such as database records, and terms from controlled vocabularies and ontologies. BioModels comprises two main branches: one is composed of models derived from literature, while the second is generated through automated processes. BioModels currently hosts over 1200 models derived directly from the literature, as well as in excess of 140,000 models automatically generated from pathway resources. This represents an approximate 60-fold growth for literature-based model numbers alone, since BioModels' first release a decade ago. This article describes updates to the resource over this period, which include changes to the user interface, the annotation profiles of models in the curation pipeline, major infrastructure changes, ability to perform online simulations and the availability of model content in Linked Data form. We also outline planned improvements to cope with a diverse array of new challenges.


Assuntos
Bases de Dados Factuais , Modelos Biológicos , Simulação por Computador , Internet
12.
Methods Mol Biol ; 1021: 189-99, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23715986

RESUMO

BioModels Database is a public online resource that allows storing and sharing of published, peer-reviewed quantitative, dynamic models of biological processes. The model components and behaviour are thoroughly checked to correspond the original publication and manually curated to ensure reliability. Furthermore, the model elements are annotated with terms from controlled vocabularies as well as linked to relevant external data resources. This greatly helps in model interpretation and reuse. Models are stored in SBML format, accepted in SBML and CellML formats, and are available for download in various other common formats such as BioPAX, Octave, SciLab, VCML, XPP and PDF, in addition to SBML. The reaction network diagram of the models is also available in several formats. BioModels Database features a search engine, which provides simple and more advanced searches. Features such as online simulation and creation of smaller models (submodels) from the selected model elements of a larger one are provided. BioModels Database can be accessed both via a web interface and programmatically via web services. New models are available in BioModels Database at regular releases, about every 4 months.


Assuntos
Algoritmos , Bases de Dados Factuais , Modelos Biológicos , Ferramenta de Busca , Animais , Simulação por Computador , Humanos , Internet , Biologia de Sistemas , Vocabulário Controlado
14.
BMC Syst Biol ; 4: 92, 2010 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-20587024

RESUMO

BACKGROUND: Quantitative models of biochemical and cellular systems are used to answer a variety of questions in the biological sciences. The number of published quantitative models is growing steadily thanks to increasing interest in the use of models as well as the development of improved software systems and the availability of better, cheaper computer hardware. To maximise the benefits of this growing body of models, the field needs centralised model repositories that will encourage, facilitate and promote model dissemination and reuse. Ideally, the models stored in these repositories should be extensively tested and encoded in community-supported and standardised formats. In addition, the models and their components should be cross-referenced with other resources in order to allow their unambiguous identification. DESCRIPTION: BioModels Database http://www.ebi.ac.uk/biomodels/ is aimed at addressing exactly these needs. It is a freely-accessible online resource for storing, viewing, retrieving, and analysing published, peer-reviewed quantitative models of biochemical and cellular systems. The structure and behaviour of each simulation model distributed by BioModels Database are thoroughly checked; in addition, model elements are annotated with terms from controlled vocabularies as well as linked to relevant data resources. Models can be examined online or downloaded in various formats. Reaction network diagrams generated from the models are also available in several formats. BioModels Database also provides features such as online simulation and the extraction of components from large scale models into smaller submodels. Finally, the system provides a range of web services that external software systems can use to access up-to-date data from the database. CONCLUSIONS: BioModels Database has become a recognised reference resource for systems biology. It is being used by the community in a variety of ways; for example, it is used to benchmark different simulation systems, and to study the clustering of models based upon their annotations. Model deposition to the database today is advised by several publishers of scientific journals. The models in BioModels Database are freely distributed and reusable; the underlying software infrastructure is also available from SourceForge https://sourceforge.net/projects/biomodels/ under the GNU General Public License.


Assuntos
Fenômenos Bioquímicos/fisiologia , Bases de Dados Factuais , Modelos Biológicos , Biologia de Sistemas/métodos , Internet , Cinética
15.
Structure ; 17(9): 1244-52, 2009 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-19748345

RESUMO

We used a protein structure prediction method to generate a variety of folds as alpha-carbon models with realistic secondary structures and good hydrophobic packing. The prediction method used only idealized constructs that are not based on known protein structures or fragments of them, producing an unbiased distribution. Model and native fold comparison used a topology-based method as superposition can only be relied on in similar structures. When all the models were compared to a nonredundant set of all known structures, only one-in-ten were found to have a match. This large excess of novel folds was associated with each protein probe and if true in general, implies that the space of possible folds is larger than the space of realized folds, in much the same way that sequence-space is larger than fold-space. The large excess of novel folds exhibited no unusual properties and has been likened to cosmological dark matter.


Assuntos
Sondas Moleculares , Dobramento de Proteína , Proteínas/química , Modelos Moleculares
16.
J R Soc Interface ; 6 Suppl 4: S405-17, 2009 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-19364720

RESUMO

A key component of any synthetic biology effort is the use of quantitative models. These models and their corresponding simulations allow optimization of a system design, as well as guiding their subsequent analysis. Once a domain mostly reserved for experts, dynamical modelling of gene regulatory and reaction networks has been an area of growth over the last decade. There has been a concomitant increase in the number of software tools and standards, thereby facilitating model exchange and reuse. We give here an overview of the model creation and analysis processes as well as some software tools in common use. Using markup language to encode the model and associated annotation, we describe the mining of components, their integration in relational models, formularization and parametrization. Evaluation of simulation results and validation of the model close the systems biology 'loop'.


Assuntos
Biologia Computacional/métodos , Biologia de Sistemas , Algoritmos , Biologia/métodos , Biotecnologia/métodos , Simulação por Computador , Redes Reguladoras de Genes , Internet , Modelos Biológicos , Modelos Teóricos , Linguagens de Programação , Software
17.
BMC Bioinformatics ; 9 Suppl 1: S13, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18315844

RESUMO

BACKGROUND: The prediction of protein structure can be facilitated by the use of constraints based on a knowledge of functional sites. Without this information it is still possible to predict which residues are likely to be part of a functional site and this information can be used to select model structures from a variety of alternatives that would correspond to a functional protein. RESULTS: Using a large collection of protein-like decoy models, a score was devised that selected those with predicted functional site residues that formed a cluster. When tested on a variety of small alpha/beta/alpha type proteins, including enzymes and non-enzymes, those that corresponded to the native fold were ranked highly. This performance held also for a selection of larger alpha/beta/alpha proteins that played no part in the development of the method. CONCLUSION: The use of predicted site positions provides a useful filter to discriminate native-like protein models from non-native models. The method can be applied to any collection of models and should provide a useful aid to all modelling methods from ab initio to homology based approaches.


Assuntos
Algoritmos , Modelos Químicos , Modelos Moleculares , Proteínas/química , Proteínas/ultraestrutura , Análise de Sequência de Proteína/métodos , Sequência de Aminoácidos , Sítios de Ligação , Simulação por Computador , Dados de Sequência Molecular , Ligação Proteica , Relação Estrutura-Atividade
18.
Proteins ; 70(4): 1610-9, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18175329

RESUMO

For many years it has been accepted that the sequence of a protein can specify its three-dimensional structure. However, there has been limited progress in explaining how the sequence dictates its fold and no attempt to do this computationally without the use of specific structural data has ever succeeded for any protein larger than 100 residues. We describe a method that can predict complex folds up to almost 200 residues using only basic principles that do not include any elements of sequence homology. The method does not simulate the folding chain but generates many thousands of models based on an idealized representation of structure. Each rough model is scored and the best are refined. On a set of five proteins, the correct fold score well and when tested on a set of larger proteins, the correct fold was ranked highest for some proteins more than 150 residues, with others being close topological variants. All other methods that approach this level of success rely on the use of templates or fragments of known structures. Our method is unique in using a database of ideal models based on general packing rules that, in spirit, is closer to an ab initio approach.


Assuntos
Modelos Moleculares , Dobramento de Proteína , Proteínas/química , Sequência de Aminoácidos , Bases de Dados de Proteínas , Peso Molecular , Conformação Proteica
19.
Philos Trans R Soc Lond B Biol Sci ; 361(1467): 413-23, 2006 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-16524830

RESUMO

Impressive progress in genome sequencing, protein expression and high-throughput crystallography and NMR has radically transformed the opportunities to use protein three-dimensional structures to accelerate drug discovery, but the quantity and complexity of the data have ensured a central place for informatics. Structural biology and bioinformatics have assisted in lead optimization and target identification where they have well established roles; they can now contribute to lead discovery, exploiting high-throughput methods of structure determination that provide powerful approaches to screening of fragment binding.


Assuntos
Biologia Computacional , Desenho de Fármacos , Avaliação Pré-Clínica de Medicamentos , Humanos , Conformação Proteica , Especificidade por Substrato
20.
J Mol Biol ; 357(5): 1669-82, 2006 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-16488431

RESUMO

The discovery that the functions of most eukaryotic gene products are mediated through multi-protein complexes makes the prediction of protein interactions one of the most important current challenges in structural biology. Rigid-body docking methods can generate a large number of alternative candidates, but it is difficult to discriminate the near-native interactions from the large number of false positives. Many different scoring functions have been developed for this purpose, but in most cases, experimental and biological information is still required for accurate predictions. We explore here the use of evolutionary restraints in evaluating rigid-body docking geometries. In order to identify potential interface residues we identify functional residues based on the comparison of observed amino acid substitutions with those predicted from local environment. The interface residues identified by this method are correctly located in 85% of the cases. These predicted interface residues are used to define distance restraints that help to score rigid-body docking solutions. We have developed the pyDockRST software, which uses the percentage of satisfied distance restraints, together with the electrostatics and desolvation binding energy, to identify correct docking orientations. This methodology dramatically improves the docking results when compared to the use of energy criteria alone, and is able to find the correct orientation within the top 20 docking solutions in 80% of the cases.


Assuntos
Substituição de Aminoácidos , Conformação Proteica , Proteínas/química , Proteínas/genética , Software , Modelos Moleculares , Ligação Proteica , Proteínas/metabolismo , Eletricidade Estática
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