RESUMO
Ascorbic acid is a nutritional small molecule essential to human life activities and health, playing a vital role in many physiological processes. Fresh fruits and beverages can provide abundant AA to maintain human metabolic balance. Therefore, it is of great significance to develop a nanomaterial with superior nanozyme activity for rapid and convenient detection of ascorbic acid (AA) in fruits and beverages. Herein, a dual-signal sensing platform based on UV-vis absorption and test strip chromaticity for the quantitative determination of AA is presented. The sensing platform is based on the horseradish peroxidase-like activity of Ni3V2O8 nanoflowers, which catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide to the blue oxide TMB (ox TMB). The ox TMB produced by the oxidation has a characteristic absorption peak at 650 nm. In the presence of AA, the blue ox TMB is reduced to colorless TMB, and the quantitative detection of AA can be achieved by detecting the decrease in intensity of the absorption peak by UV-Vis spectrophotometry. Under the optimal experimental conditions, the sensing platform exhibited excellent sensitivity and selectivity. A wide linear range of 0.1 µM to 40 µM with a detection limit of 0.032 µM was obtained. The linear equation is ΔA = 0.02513c + 0.1164 with a correlation coefficient of 0.9979. It showed excellent properties in the detection of real samples of fruit juices and beverages, meanwhile, a method for the rapid detection of AA based on chromaticity change of test strips was constructed with high sensitivity and convenience. The linearity range for the ascorbic acid was 1-50 µM with LOD of 0.42 µM. The developed sensing platform has the capability to quickly and accurately detect ascorbic acid (AA) in fresh fruits and beverages. This proposed method offers a new and promising approach for the rapid and cost-effective detection of ascorbic acid, which has a wide range of potential applications.
RESUMO
Aflatoxin B1 (AFB1) is a group of heterocyclic aromatic hydrocarbon secondary metabolites, which are the most toxic among the known fungal toxins. Therefore, it becomes particularly important to develop sensitive, accurate, rapid and simple methods for the detection of AFB1. In this work, a method of constructing aflatoxin aptasensor with black phosphorus nano sheet loaded with gold nanoparticles as electrode modification material, Ce-metal organic framework (MOF) material as signal label and prism DNA nano structure modified electrode as recognition interface is proposed. The hybridization between prism DNA and primer probe was used to trigger rolling circle amplification (RCA) on the electrode surface, and then the complementary chain modified with Au NPs@Ce-MOF is bound to the amplification chain to provide electrochemical signals. In the range of 0.024-100 ng mL-1, the response current showed a good linear relationship with the logarithm of aflatoxin concentration, the linear equation was I = 6.4181 lg c + 11.975 with the linear correlation coefficient of 0.9973, and the detection limit was 1.48 pg mL-1 (S/N = 3).
