RESUMO
A new calibration method for pure rotational Raman lidar temperature measurement is described in this work. The method forms a temperature-dependent term in the intensity ratio, which is calculable with the radiosonde data, and then derives a calibration factor, with which the temperature is retrievable from the lidar return. The method is demonstrated and compared with existing methods through simulations and experiments. Results of the comparison show that the proposed method could provide more accurate calibrations under low signal-to-noise ratio conditions and could thus reduce the lidar performance requirement for temperature retrieval.
RESUMO
Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geographical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat (ISSR) markers were conducted to detect S. ningpoensis from different origins. A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region (SCAR) markers were designed to distinguish geo-authentic S. ningpoensis from others. This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.
Assuntos
Plantas Medicinais/genética , Scrophularia/genética , Sequência de Bases , China , Clonagem Molecular , Primers do DNA/genética , DNA de Plantas/genética , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Marcadores Genéticos , Medicina Tradicional Chinesa , Repetições Minissatélites , Reação em Cadeia da PolimeraseRESUMO
The guanylhydrazone of 2-(4-chlorobenzyloxy)-5-bromobenzaldehyde, 1, with an IC(50) of 840 nM against the CCR5 receptor was identified using high-throughput screening. Optimization efforts led to the discovery of a novel piperidine series of CCR5 antagonists. In particular, the 4-hydroxypiperidine derivative, 6k, had improved potency against CCR5, and was a starting point for further optimization. SAR elaboration using parallel synthesis led to the identification of 10h, a potent CCR5 antagonist with an IC(50) of 11 nM.
