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1.
J Tradit Chin Med ; 41(6): 935-942, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34939390

RESUMO

OBJECTIVE: To evaluate the molecular mechanism underlying the beneficial effect of Bushen Qiangjin capsule (BSQJ), a Traditional Chinese Medicine, on knee osteoarthritis (KOA). METHODS: In the present study, 32 female Sprague-Dawley rats were randomly divided into four groups: control, KOA, high-dose BSQJ (H-BSQJ), and low-dose BSQJ (L-BSQJ). After successfully establishing the KOA model by intra-articular injection of papain, H-BSQJ and L-BSQJ groups were intragastrically administered 0.243 and 0.122 g/kg BSQJ, respectively, daily for 6 weeks. At the end of the experiment, knee articular cartilage tissues of rats were collected for evaluation by hematoxylin and eosin staining, Safranin O-Fast Green staining, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. Serum interleukin-1α and tumor necrosis factor-α levels of rats were detected with an enzyme-linked immunosorbent assay method. Gene expression of Wnt-4, α-catenin, Frizzled-2, glycogen synthase kinase-3ß (GSK-3ß), cysteinyl aspartate-specific proteinases 3 and 9 (caspases 3 and 9), collagen type II alpha 1 (Col2a1), and matrix metalloproteinases 1 and 13 (MMP-1 and MMP-3) of rat knee articular cartilage was quantified by reverse transcription-quantitative polymerase chain reaction analysis. Wnt-4, α-catenin, Frizzled-2, GSK-3ß, cleaved caspase-3, and cleaved caspase-9 protein expression in rat knee articular cartilage was determined by western blot analysis. RESULTS: BSQJ obviously reduced pathological damage and matrix degradation of articular cartilage in KOA rats. Compared with the KOA group, H-BSQJ rats exhibited downregulated mRNA and protein expression of Wnt-4, ß-catenin, Frizzled-2,and caspase-3, as well as upregulated mRNA and protein expression of GSK-3α. In addition, H-BSQJ significantly increased mRNA expression of Col2a1 and decreased mRNA expression of MMP-1 and MMP-13. CONCLUSION: BSQJ exerted a beneficial effect on KOA by a mechanism involving downregulation of the Wnt/α-catenin pathway, which inhibited both cartilage extracellular matrix degradation and chondrocyte apoptosis to ameliorate KOA in rats.


Assuntos
Cartilagem Articular , Osteoartrite do Joelho , Animais , Cartilagem Articular/metabolismo , Feminino , Glicogênio Sintase Quinase 3 beta/metabolismo , Osteoartrite do Joelho/tratamento farmacológico , Osteoartrite do Joelho/genética , Osteoartrite do Joelho/metabolismo , Papaína/metabolismo , Papaína/farmacologia , Ratos , Ratos Sprague-Dawley , Via de Sinalização Wnt , alfa Catenina/metabolismo
2.
Int J Infect Dis ; 65: 81-84, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29031606

RESUMO

Noroviruses are a common cause of acute gastroenteritis outbreaks in institutions including schools and kindergartens around the world. An outbreak caused by GII.P16-GII.2 norovirus in a kindergarten in Lianyungang, Jiangsu Province, China is reported here. An epidemiological investigation was conducted, and pathogen detection was performed. The descriptive analysis indicated that this outbreak in middle class 1 had a point source. Twenty cases of acute gastroenteritis occurred in this class within a period of 8.5h; the attack rate was 52.6% (20/38). Airborne transmission via the air conditioning unit in a confined restroom could have played a critical role in this outbreak. Sequence analysis of GII-positive samples confirmed that the norovirus GII.P16-GII.2 variant was the etiological agent of this outbreak.


Assuntos
Ar Condicionado , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/transmissão , Surtos de Doenças , Gastroenterite/epidemiologia , Norovirus/isolamento & purificação , Doença Aguda , Poluição do Ar em Ambientes Fechados , Infecções por Caliciviridae/diagnóstico , Pré-Escolar , China/epidemiologia , Fezes/virologia , Feminino , Gastroenterite/diagnóstico , Gastroenterite/virologia , Humanos , Incidência , Masculino , Instituições Acadêmicas , Manejo de Espécimes
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