Contribution of serum IL-4 and IgE to the early prediction of allergic reactions induced by chlorogenic acid.

Chlorogenic acid (CA) is one of the active ingredients in some Chinese herbal injections, which may cause allergic reactions in clinic therapy. However, the criterion of test for allergen had not been employed in current Pharmacopeia of United States, European Pharmacopeia, Japanese Pharmacopeia and British Pharmacopeia. In order to find a new way to predict allergic reactions induced by CA earlier, the guinea pigs were sensitized successively by injecting CA intravenously once a day for three times, the results were compared that of Chinese Pharmacopeia by injecting CA intraperitoneally once every other day for three times, serum IL-4 and total IgE were detected by method of enzyme linked immunosorbent assay (ELISA) before guinea pigs were challenged once by injecting the same drug intravenously. The time-effectiveness and dose-effect of allergic reactions induced by CA were also studied. We found that contents of serum IL-4 and total IgE increased significantly before guinea pigs were challenged, either in D8 after intravenous sensitization (1.5 g/l CA, 0.5 ml) or in D14 and D21 after intraperitoneal sensitization (1.5 g/l CA, 0.5 ml), and allergic reactions occurred in all guinea pigs after challenged once by injecting CA (1.5 g/l, 1.0 ml) intravenously. It provides a new way to predict whether CA (or Chinese herbal injections contained CA) can provoke allergic reactions by detecting serum IL-4 and total IgE earlier; the examination period is reduced by 1-2 weeks. It has a good prospect of application in drug emergency test.

[Determination of organochlorine pesticide residues in Microcos paniculata by GC].

OBJECTIVE: To establish a method for determination 9 organochlorine pesticide residue in Microcos paniculata by gas chromatography. METHODS: The sample was infiltrated with water and extracted with hexane by ultrasonic. The extract solvent was purified by concentrated sulphuric acid. DB-1701 capillary column was used to separate the sample. GC-ECD was applied to determine the residues of organochlorine pesticide. RESULTS: The percentage recoveries were ranged from 78.1% to 97.8% and RSD were from 2.4% to 8.8%. CONCLUSION: The method established is quick, simple and low in cost.

[Study on HPLC fingerprint characteristic analysis of Cordyceps sinensis and its similar products].

OBJECTIVE: To study on HPLC fingerprint characteristic analysis of Cordyceps sinensis and its similar products. METHODS: To determinate 13 samples of Cordyceps sinensis and its similar products by HPLC, and analyze the HPLC results with similar appraisal method and graphical methods of multivariate sample in two dimensional plane such as the methods of profile, radar chart and constellation graph. RESULTS: The similar appraisal method might synthesize the similar degree in quantification, while the graphical methods such as profile graph, radar chart and constellation graph could show more details about the classification and the characteristic of varieties directly. CONCLUSIONS: We recommend the combined application of similar appraisal method and the graphical methods due to its advantages on the judgment and characteristic analysis of fingerprint.

[Determination of organochlorine pesticides redidues in traditional Chinese medicine including ginseng or American ginseng].

OBJECTIVE: To determine the residues of 19 organochlorine pesticides in traditional chinese medicine (TCM) including Radix ginseng or Radix panacis quinquefolii by capillary gas chromatograrhy. METHODS: The samples were extracted with ethyl acetate by ultrasonic, then cleaned up by a column packed with Florisil. The capillary column was DB-1701 (30 m x 0.32 m, 0.25 microm). The electron capture detector (ECD) was used. The pesticide residues were calculated by external standard method. RESULTS: The average recoveries and RSD ranged from 70.4% to 125.0% and 3.16% to 9.86%. CONCLUSION: The method is rapid, simple and accurate. It can be used to determine organochlorine pesticide residues in TCM.