The observation of atmospheric HONO by wet-rotating-denuder ion chromatograph in a coastal city: Performance and influencing factors.

Due to the significance of atmospheric HONO as a reservoir for radicals and the presence of substantial unknown sources of HONO, there is a pressing need for accurate and consistent measurement of its concentration. In this study, we compared the measurements obtained from the monitor for aerosols and gases in ambient air (MARGA) based on wet chemical method with those from the incoherent broadband cavity-enhanced absorption spectroscopy (IBBCEAS) based on optical method to assess the suitability of the MARGA instrument for accurate HONO detection. The diurnal patterns obtained by the two instruments are similar, with peaks at 8 a.m. and lows at 5 p.m. Over the course of the observation period, it was often observed that HONO concentrations recorded by the MARGA instrument consistently exceeded those obtained through the IBBCEAS technique, accounting for approximately 91.33% of the total observation time. Throughout the entire observation period, the R2 value between the two instruments was 0.49, indicating relatively good correlation. However, with a slope of only 0.27, it suggests poor agreement between the two instruments. Furthermore, the R2 and slopes between the two instruments vary with the seasons and day-night. The larger the quartile values of NO2, NH3, and BC, the greater the slopes of both MARGA and IBBCEAS instruments, and the higher the concentrations of NO2, NH3, and BC (indicator of semivolatile oxidizable hydrocarbons), the greater the differences between the two instruments, all indicating that NH3 may promote the reaction of NO2 with semivolatile oxidizable hydrocarbons to produce HONO. The O3 with its strong oxidizing properties may cause underestimation in the MARGA instrument by oxidizing NO2- to NO3- in the absorbing solution. It is challenging to derive a universal correction formula due to the interference of various chemical substances. Hence, MARGA should not be used for HONO research in the future.

Comparative in vitro toxicological effects of water-soluble and insoluble components of atmospheric PM2.5 on human lung cells.

Fine particulates in city air significantly impact human health, but the hazardous compositional mechanisms are still unclear. Besides the toxicity of environmental PM2.5 to in vitro human lung epithelial cells (A549), the independent cytotoxicity of PM2.5-bound water-soluble (WS-PM2.5) and water-insoluble (WIS-PM2.5) fractions were also compared by cell viability, oxidative stress (reactive oxygen species, ROS), and inflammatory injury (IL-6 and TNF-α). The cytotoxicity of PM2.5 varied significantly by sampling season and place, with degrees greater in winter and spring than in summer and autumn, related to corresponding trend of air PM2.5 level, and also higher in industrial than urban site, although their PM2.5 pollution levels were comparable. The PM2.5 bound metals (Ni, Cr, Fe, and Mn) may contribute to cellular injury. Both WS-PM2.5 and WIS-PM2.5 posed significant cytotoxicity, that WS-PM2.5 was more harmful than WIS-PM2.5 in terms of decreasing cell viability and increasing inflammatory cytokines production. In particular, industrial samples were usually more toxic than urban samples, and those from summer were generally less toxic than other seasons. Hence, in order to mitigate the health risks of PM2.5 pollution, the crucial targets might be components of heavy metals and soluble fractions, and sources in industrial areas, especially during the cold seasons.

Altered metabolome and microbiome associated with compromised intestinal barrier induced hepatic lipid metabolic disorder in mice after subacute and subchronic ozone exposure.

Exposure to ozone has been associated with metabolic disorders in humans, but the underlying mechanism remains unclear. In this study, the role of the gut-liver axis and the potential mechanism behind the metabolic disorder were investigated by histological examination, microbiome and metabolome approaches in mice during the subacute (4-week) and subchronic (12-week) exposure to 0.5 ppm and 2.5 ppm ozone. Ozone exposure resulted in slowed weight gain and reduced hepatic lipid contents in a dose-dependent manner. After exposure to ozone, the number of intestinal goblet cells decreased, while the number of tuft cells increased. Tight junction protein zonula occludens-1 (ZO-1) was significantly downregulated, and the apoptosis of epithelial cells increased with compensatory proliferation, indicating a compromised chemical and physical layer of the intestinal barrier. The hepatic and cecal metabolic profiles were altered, primarily related to lipid metabolism and oxidative stress. The abundance of Muribaculaceae increased dose-dependently in both colon and cecum, and was associated with the decrease of metabolites such as bile acids, betaine, and L-carnitine, which subsequently disrupted the intestinal barrier and lipid metabolism. Overall, this study found that subacute and subchronic exposure to ozone induced metabolic disorder via disturbing the gut-liver axis, especially the intestinal barrier. These findings provide new mechanistic understanding of the health risks associated with environmental ozone exposure and other oxidative stressors.

Meteorological impacts on the unexpected ozone pollution in coastal cities of China during the unprecedented hot summer of 2022.

Surface ozone pollution under climate warming has become a serious environmental issue. In the summer of 2022, abnormal warming spread over most of the Northern Hemisphere and resulted in the abnormal increase in O3 concentrations. In this study, we focused on the coastal cities in China and investigated the O3 trends in July during 2015 to 2022. Four regions with different locations and emission levels were selected for comparison. A significant increase of O3 concentration in July 2022 were observed in the southern coastal cities (16.7-22.8 µg m-3) while the opposite characteristics were found in the northern coastal cities (decrease of 0.26-2.18 µg m-3). The results indicated various distribution patterns of the O3 concentrations responded to heat wave across China. The weakening of East Asian summer monsoon, extension of the western Pacific subtropical high, significant warming, stronger solar radiation, lower relative humidity, less rainfall and sinking motion of atmosphere in 2022 were beneficial for O3 generation and accumulation in the southern coastal areas. Meteorological changes in July 2022 could lead to an increase of 15.6 % in O3 concentrations in southern coastal cities compared to that in 2015-2021, based on the analysis of machine learning. Air temperature was the main contributor to high O3 concentrations in the coast of Fujian province, while other coastal cities depended on relative humidity. This study indicated the challenge of O3 pollution control in coastal areas under global warming, especially in extreme heat wave events.

PM2.5 air quality and health gains in the quest for carbon peaking: A case study of Fujian Province, China.

China faces a dual challenge of improving air quality and reducing greenhouse gas (GHG) emissions. Stringent clean air actions gradually narrow the end-of-pipe (EOP) pollution control potential. Meanwhile, pursuing carbon peaking will reduce air pollution and health risks. However, the impact on air quality and health gains in individual Chinese provinces has not been assessed with a specific focus on local policies. Here, typical shared socio-economic pathways (SSPs) and local policies (i.e., business as usual, BAU; end-of-pipe controls, EOP; co-control mitigation, CCM) are combined to set three scenarios (i.e., BAU-SSP3, EOP-SSP4, CCM-SSP1). Under these three scenarios, we couple the Low Emissions Analysis Platform (LEAP) model, an air quality model and health risk assessment methodology to evaluate the characteristics of carbon peaking in Fujian Province. PM2.5 air quality and impacts on public health are assessed, using the metric of the deaths attributable to PM2.5 pollution (DAPP). The results show that energy-related CO2 emissions will only peak before 2030 in the CCM-SSP1 scenario. In this context, air pollutant emission pathways reveal that mitigation is limited under the EOP-SSP4 scenario, necessitating further mitigation under the CCM-SSP1 scenario. The annual average PM2.5 level is projected to be 16.5 µg·m-3 in 2035 with a corresponding decrease in DAPP of 297 (95 % confidence intervals: 217-308) compared with that of 2020. Despite the significant improvements in PM2.5 air quality and health gains under the CCM-SSP1 scenario, reaching the 5 µg·m-3 target of the World Health Organization (WHO) remains difficult. Furthermore, population aging will require stronger PM2.5 mitigation to enhance health gains. This study provides a valuable reference for other developing regions to co-control air pollution and GHGs.

New Insights on the Formation of Nucleation Mode Particles in a Coastal City Based on a Machine Learning Approach.

Atmospheric particles have profound implications for the global climate and human health. Among them, ultrafine particles dominate in terms of the number concentration and exhibit enhanced toxic effects as a result of their large total surface area. Therefore, understanding the driving factors behind ultrafine particle behavior is crucial. Machine learning (ML) provides a promising approach for handling complex relationships. In this study, three ML models were constructed on the basis of field observations to simulate the particle number concentration of nucleation mode (PNCN). All three models exhibited robust PNCN reproduction (R2 > 0.80), with the random forest (RF) model excelling on the test data (R2 = 0.89). Multiple methods of feature importance analysis revealed that ultraviolet (UV), H2SO4, low-volatility oxygenated organic molecules (LOOMs), temperature, and O3 were the primary factors influencing PNCN. Bivariate partial dependency plots (PDPs) indicated that during nighttime and overcast conditions, the presence of H2SO4 and LOOMs may play a crucial role in influencing PNCN. Additionally, integrating additional detailed information related to emissions or meteorology would further enhance the model performance. This pilot study shows that ML can be a novel approach for simulating atmospheric pollutants and contributes to a better understanding of the formation and growth mechanisms of nucleation mode particles.

Sources appointment and health risks of PM2.5-bound trace elements in a coastal city of southeastern China.

To gain a comprehensive understanding of sources and health risks of trace elements in an area of China with high population densities and low PM2.5 concentrations, 15 trace elements (Al, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Sn, Ba, Pb) in PM2.5 were monitored from December 2020 to November 2021 in a representative city, Xiamen. The concentrations of trace elements in Xiamen displayed an obvious seasonal variation and were dominated by K, Fe, Al, Ca and Zn. Based on Positive Matrix Factorization analysis, source appointment revealed that the major sources of trace elements in Xiamen were traffic, dust, biomass and firework combustion, industrial manufacture and shipping emission. According to health risk assessment combined with the source appointment results, it indicated that the average noncarcinogenic risk was below the threshold and cancer risk of four hazardous metals (Cr, Ni, As, Pb) exceeded the threshold (10-6). Traffic-related source had almost half amount of contribution to the health risk induced by PM2.5-bound trace elements. During the dust transport period or Spring Festival period, the health risks exceeded an acceptable threshold even an order of magnitude higher, suggesting that the serious health risks still existed in low PM2.5 environment at certain times. Health risk assessment reminded that the health risk reduction in PM2.5 at southeastern China should prioritize traffic-related hazardous trace elements and highlighted the importance of controlling vehicles emissions in the future.

Molecular Composition of Anthropogenic Oxygenated Organic Molecules and Their Contribution to Organic Aerosol in a Coastal City.

Organic aerosols (OA) have gained attention as a substantial component of atmospheric aerosols owing to their impact on atmospheric visibility, climate, and human health. Although oxygenated organic molecules (OOMs) are essential contributors to OA formation, the sources, transformations, and fates of the OOMs are not fully understood. Herein, anthropogenic OOMs (AOOMs), anthropogenic volatile organic compounds (AVOCs), and OA were concurrently measured in Xiamen, a coastal city in southeastern China. Our results show that the AOOMs exhibited a high nitrogen content (76%) and a low oxidation degree. Strong photochemical processes of aromatic VOCs were the predominant sources of AOOMs. Also, NOx concentrations and the occurrence of multigeneration OH radical oxidations were the critical factors that might influence the formation of AOOMs. Finally, the newly developed aerosol dynamic model's results show that more than 35% of the OA mass growth rate is attributed to the gas-particle partitioning of AOOMs. Further sensitivity testing demonstrates that the contribution of AOOMs to OA growth is significantly enhanced during high-particulate-concentration periods, especially under low-temperature conditions. This study emphasizes the vital role of photochemically produced AOOMs derived from AVOCs in OA growth in a coastal urban atmosphere.

Photochemistry in the urban agglomeration along the coastline of southeastern China: Pollution mechanism and control implication.

The concentrations of ground-level ozone (O3) in China have undergone a rapid increase in recent years, resulting in adverse impacts on the air quality and climate change. However, limited research has been conducted on the coastal urban agglomerations with increasingly serious O3 pollution. Therefore, in order to better understand in situ photochemistry, comprehensive field observations of O3 and its precursors, coupled with the model simulation, were conducted in autumn of 2019 at six sites in an urban agglomeration along the coastline of southeastern China. Results indicated that O3 pollution in the southern part of the urban agglomeration was more severe than that in the northern part, due to higher levels of O3 precursors and stronger atmospheric oxidation capacity (AOC) in the southern regions. Oxygenated volatile organic compounds (OVOCs), NO2, and CO dominated the total OH reactivity, and the site-average daytime Ox (O3 + NO2) increments correlated well (R2 = 0.94) with the total OH reactivity of CO and VOCs at these sites except for Quanzhou, where industrial emissions (35.1 %) and solvent usages (33.7 %) dominated the VOC sources. However, vehicle exhausts (31.1 %) were the most predominant contributors to the VOC sources at other sites. The results of model simulations showed that net O3 formation rates were larger at the southern sites. Furthermore, O3 production was mainly controlled by VOCs at most sites, but co-limited by VOCs and NOx at Quanzhou. The most significant VOC groups contributing to O3 formation were aromatics and alkenes, with m/p-xylene, toluene, propene, and ethene being the main contributors at these sites. This study offers a more comprehensive understanding of the characteristics and formation of photochemical pollutions on the scale of the urban areas, indicating the critical need to reduce VOC emissions as a means of mitigating their photochemical effects.

The role of glucocorticoids in increasing cardiovascular risk.

Introduction: Different studies provide conflicting evidence regarding the potential for glucocorticoids (GCs) to increase the risk of cardiovascular diseases. This study performed a systematic review and meta-analysis to determine the correlation between GCs and cardiovascular risk, including major adverse cardiovascular events (MACE), death from any cause, coronary heart disease (CHD), heart failure (HF), and stroke. Methods: We performed a comprehensive search in PubMed and Embase (from inception to June 1, 2022). Studies that reported relative risk (RR) estimates with 95% confidence intervals (CIs) for the associations of interest were included. Results: A total of 43 studies with 15,572,512 subjects were included. Patients taking GCs had a higher risk of MACE (RR = 1.27, 95% CI: 1.15-1.40), CHD (RR = 1.25, 95% CI: 1.11-1.41), and HF (RR = 1.92, 95% CI: 1.51-2.45). The MACE risk increased by 10% (95% CI: 6%-15%) for each additional gram of GCs cumulative dose or by 63% (95% CI: 46%-83%) for an additional 10 µg daily dose. The subgroup analysis suggested that not inhaled GCs and current GCs use were associated with increasing MACE risk. Similarly, GCs were linked to an increase in absolute MACE risk of 13.94 (95% CI: 10.29-17.58) cases per 1,000 person-years. Conclusions: Administration of GCs is possibly related with increased risk for MACE, CHD, and HF but not increased all-cause death or stroke. Furthermore, it seems that the risk of MACE increased with increasing cumulative or daily dose of GCs.

The effect of nitrous acid (HONO) on ozone formation during pollution episodes in southeastern China: Results from model improvement and mechanism insights.

Two ozone (O3) processes, summer episode dominated by local production and autumn episode dominated by regional transport, were chosen to investigate the role of HONO in different pollution processes. Meteorological conditions, diurnal variation of O3, potential source contribution factor (PSCF) analysis, concentration weighted trajectory (CWT) models, and the distribution of the eight-hour maximum values of O3 on mainland China all prove that summer O3 was mainly locally generated while autumn O3 episode was more susceptible to regional transport. The gaps between observations and simulation results with the default HONO chemistry in Master Chemical Mechanism (MCM) of Observation Based Model (OBM) were higher in summer episode (0.58 ppb) than autumn episode (0.37 ppb). Although we implemented nine additional sources in the model to revise the HONO chemistry, the simulated values were still lower than the observed values. HONO promoted O3 production by accelerating the reaction of HO2 + NO and RO2 + NO, and promoted loss of O3 by the reaction of OH + NO2 and RO2 + NO2. The net production rate of O3 with HONO constraint increased by 28.50 % in summer and 22.43 % in autumn, which also indicated that HONO played more important role in the O3 production in summer. The difference of NOx of daily RIR between the cases with and without HONO constraint was higher in summer O3 episode (0.15 %/%) than that in autumn O3 episode (0.09 %/%), the same as to VOCs with -0.20 %/% in summer O3 episode and - 0.14 %/% in autumn O3 episode, which indicated that the presence or absence of the HONO constraint has a greater impact on the RIR value in the case of dominant local generation. In brief, the O3 sensitivity would be more favorable for VOCs without HONO constrained in the model, which would inevitably mislead policy makers to develop efficient policies to control O3 pollution.

Pollution mechanisms and photochemical effects of atmospheric HCHO in a coastal city of southeast China.

Formaldehyde (HCHO) is a vital reactive carbonyl compound, which plays an important role in the photochemical process and atmospheric oxidation capacity. However, the current studies on the quantification of HCHO impacts on atmospheric photochemistry in southeast coastal areas of China with an obvious upward trend of ozone remain scarce and unclear, thus limiting the full understanding of formation mechanism and control strategy of photochemical pollution. Here, systematic field campaigns were conducted at a typical coastal urban site with good air quality to reveal HCHO mechanism and effects on O3 pollution mechanism during spring and autumn, when photochemical pollution events still frequently appeared. Positive Matrix Factorization model results showed that secondary photochemical formation made the largest contributions to HCHO (69 %) in this study. Based on the photochemical model, the HCHO loss rates in autumn were significantly higher than those in spring (P < 0.05), indicating that strong photochemical conditions constrain high HCHO levels in certain situations. HCHO mechanism increased the ROx concentrations by 36 %, and increased net O3 production rates by 31 %, manifesting that the reduction of HCHO and its precursors' emissions would effectively mitigate O3 pollution. Therefore, the pollution characteristics and photochemical effects of HCHO provided significant guidance for future photochemical pollution control in relatively clean areas.

Distinct responses of airborne abundant and rare microbial communities to atmospheric changes associated with Chinese New Year.

Airborne microorganisms, including pathogens, would change with surrounding environments and become issues of global concern due to their threats to human health. Microbial communities typically contain a few abundant but many rare species. However, how the airborne abundant and rare microbial communities respond to environmental changes is still unclear, especially at hour scale. Here, we used a sequencing approach based on bacterial 16S rRNA genes and fungal ITS2 regions to investigate the high time-resolved dynamics of airborne bacteria and fungi and to explore the responses of abundant and rare microbes to the atmospheric changes. Our results showed that air pollutants and microbial communities were significantly affected by human activities related to the Chinese New Year (CNY). Before CNY, significant hour-scale changes in both abundant and rare subcommunities were observed, while only abundant bacterial subcommunity changed with hour time series during CNY. Air pollutants and meteorological parameters explained 61.5%-74.2% variations of abundant community but only 13.3%-21.6% variations of rare communities. These results suggested that abundant species were more sensitive to environmental changes than rare taxa. Stochastic processes predominated in the assembly of abundant communities, but deterministic processes determined the assembly of rare communities. Potential bacterial pathogens during CNY were the highest, suggesting an increased health risk of airborne microbes during CNY. Overall, our findings highlighted the "holiday effect" of CNY on airborne microbes and expanded the current understanding of the ecological mechanisms and health risks of microbes in a changing atmosphere.

Health threat of PM2.5-bound trace elements exposure on asthma hospital admission: A time-stratified case-crossover study.

Environmental pollution of trace elements has become of main concern due to the adverse effects. To estimate the impact of PM2.5-bound trace elements on human health, a time-stratified case-crossover study has been designed to examine the short-term associations between 28 elements and asthma hospitalizations from January 2019 to November 2021 in Xiamen, China. This research summarized the major components that pose health risks in different seasons and took risk assessment for different groups. We found that an inter-quartile range (IQR) increase of Ca, Fe, Mn, Pd, Si, and Ti was positively associated with the incidence of asthma in the lag of 0-4 days. In winter, the elements that predisposed the population to asthma attack were the most, such as Al, Ba, Ca, Cr, Cu, Fe, K, Mn, Pd, Si, and Ti, and these elements are at higher risk for longer periods of time. The discrepancy in risk levels and major elements of asthma diagnosis among various age groups were also found in this work. Our results provided insights into the development of specific policies to reduce the risk of asthma attacks due to exposure to PM2.5-bound trace elements.

Intradiscal Autologous Platelet-Rich Plasma Injection for Discogenic Low Back Pain: A Clinical Trial.

Background: Platelet-rich plasma (PRP) contains high concentrations of growth factors and cytokines and may promote healing and tissue formation and exert anti-inflammatory effects. PRP has been shown to improve intervertebral disc degeneration in vivo and in vitro. This study is aimed at evaluating the effectiveness of autologous PRP on discogenic low back pain (DLBP) at 48 weeks postinjection in patients who received a single intradiscal injection. Methods: All patients received a single intradiscal injection of PRP in a prospective trial. The pain scores, lumbar function, and adverse events were assessed at 1 week, 4 weeks, 8 weeks, 12 weeks, 24 weeks, and 48 weeks postinjection and compared to the preinjection values (0 weeks). Results: Data were analysed from 31 patients with a 94% follow-up rate. Compared to preinjection, pain and lumbar function were significantly improved, and there were significant differences (P < 0.05) over the 48-week follow-up. Twenty-two (71%) patients were classified as successes after the intradiscal injection of PRP. One patient received surgery at two weeks postinjection due to intervertebral discitis. Conclusions: Intradiscal injection of PRP can significantly relieve pain sensation and improve lumbar function in patients with DLBP over a 48-week follow-up period. Further randomized controlled clinical trials are needed to assess the effects of this injection therapy.

Improved atmospheric mercury simulation using updated gas-particle partition and organic aerosol concentrations.

The gaseous or particulate forms of divalent mercury (HgII) significantly impact the spatial distribution of atmospheric mercury concentration and deposition flux (FLX). In the new nested-grid GEOS-Chem model, we try to modify the HgII gas-particle partitioning relationship with synchronous and hourly observations at four sites in China. Observations of gaseous oxidized Hg (GOM), particulate-bound Hg (PBM), and PM2.5 were used to derive an empirical gas-particle partitioning coefficient as a function of temperature (T) and organic aerosol (OA) concentrations under different relative humidity (RH). Results showed that with increasing RH, the dominant process of HgII gas-particle partitioning changed from physical adsorption to chemical desorption. And the dominant factor of HgII gas-particle partitioning changed from T to OA concentrations. We thus improved the simulated OA concentration field by introducing intermediate-volatility and semi-volatile organic compounds (I/SVOCs) emission inventory into the model framework and refining the volatile distributions of I/SVOCs according to new filed tests in the recent literatures. Finally, normalized mean biases (NMBs) of monthly gaseous element mercury (GEM), GOM, PBM, WFLX were reduced from -33%-29%, 95%-300%, 64%-261%, 117%-122% to -13%-0%, -20%-80%, -31%-50%, -17%-23%. The improved model explains 69%-98% of the observed atmospheric Hg decrease during 2013-2020 and can serve as a useful tool to evaluate the effectiveness of the Minamata Convention on Mercury.

Particle number size distribution and new particle formation in Xiamen, the coastal city of Southeast China in wintertime.

New particle formation (NPF) has a great impact on regional and global climate, air quality and human health. This study uses a Scanning Mobility Particle Sizer (SMPS) for simultaneous measurement of particle number size distribution (PNSD) in wintertime to investigate NPF in the coastal city of Xiamen. The mean particle number concentration, surface area concentration and volume concentration were 7.25 × 103 cm-3, 152.54 µm2 cm-3, and 4.03 µm3 cm-3, respectively. Particle number concentration was mainly influenced by the nucleation mode and the Aitken mode, whereas the main contributor to particle surface area concentration and volume concentration was accumulation mode particles. The frequency of NPF events occurred was around 41.4% in December 2019. The typical growth rates of new formed particles were 1.41-2.54 nm h-1, and the observed formation rates were 0.49-1.43 cm-3 s-1. A comparative analysis of conditions between event and non-event days was performed. The results emphasized that air temperature, UV radiation and relative humidity were the most decisive meteorological factors, and NPF events usually occurred under clean atmospheric conditions with low PM concentrations. Although condensation sink was high when NPF event occurred, the level of SO2 and O3 concentration was also high.

Integrated metabolomic and transcriptomic analysis identifies benzo[a]pyrene-induced characteristic metabolic reprogramming during accumulation of lipids and reactive oxygen species in macrophages.

Polycyclic aromatic hydrocarbon exposure is a major risk factor for cardiovascular diseases. Macrophage lipid accumulation is a characteristic molecular event in the pathophysiology of cardiovascular diseases. Metabolic reprogramming is an intervention target for diseases and toxic effects of environmental pollutants. However, comprehensive metabolic reprogramming related to BaP-induced macrophage lipid accumulation is currently unexplored. Therefore, metabolomics and transcriptomics were conducted to unveil relevant metabolic reprogramming in BaP-exposed macrophages, and to discover potential intervention targets. Metabolomics revealed that most amino acids, nucleotides, monosaccharides, and organic acids were significantly decreased, while most fatty acids and steroids accumulated in BaP-exposed macrophages. Transcriptomics showed that fatty acid synthesis and oxidation, and steroid synthesis and export were decreased, while import of fatty acids and steroids was increased, indicating potential roles of lipid transport in macrophage lipid accumulation following BaP exposure. Meanwhile, alanine, aspartate and glutamate metabolism, branched-chain amino acid degradation, nucleotide synthesis, monosaccharide import, pentose phosphate pathway, citrate synthesis, and glycolysis were decreased, while nucleotide degradation was increased, thus inducing decreases in most amino acids, nucleotides, monosaccharides, and organic acids in BaP-exposed macrophages. Additionally, increases in oxidative stress and the activation of antioxidant systems were observed in BaP-exposed macrophages, which was evinced by increases in reactive oxygen species, and the activation of Fenton reaction, Vdac2/3, Sod2, and Nrf2. Moreover, BaP-induced accumulation of reactive oxygen species and lipids in macrophages could be abolished by epigallocatechin-3-gallate. Quantitative PCR showed that BaP exposure activated aryl hydrocarbon receptor signaling and promoted the proinflammatory phenotype in macrophages, and these effects were inhibited or even abolished by the separate treatment with epigallocatechin-3-gallate or CH-223191, suggesting the regulatory role of aryl hydrocarbon receptor signaling in BaP-induced toxic effects. This study provides novel insights into the toxic effects of polycyclic aromatic hydrocarbons on macrophage metabolism and potential intervention targets.

Characteristics of secondary organic aerosols tracers in PM2.5 in three central cities of the Yangtze river delta, China.

Secondary organic aerosols (SOA) are important atmospheric pollutants that affect air quality, radiation, and human health. In this study, 14 typical SOA tracers were measured in PM2.5 collected from three central cities of the Yangtze River Delta (YRD) region in the winter of 2014 and the summer of 2015. Among the determined SOA tracers, α/ß-pinene SOA tracers contributed 55.9%, followed by isoprene SOA tracers (33.7%), anthropogenic benzene SOA tracer (6.4%) and ß-caryophyllene SOA tracer (4.0%). There was no significant difference in the concentration of individual SOA tracers among the three cities (p > 0.05), indicating a high degree of regional consistency. The concentrations of isoprene, α/ß-pinene, and toluene SOA tracers were significantly higher in summer than in winter. A correlation of SOA tracers with temperature implies that the isoprene and α/ß-pinene SOA tracers in summer were greatly boosted by plant emissions and the high DHOPA in summer could be attributed to evaporation of paint and solvent. In contrast, the elevated ß-caryophyllene SOA tracer in winter was likely associated with active biomass burning. Furthermore, we observed a close correlation of summer isoprene and α/ß-pinene SOA tracers with sulfate only in Shanghai, which verifies that biogenic SOA formation was facilitated by high concentration of sulfate. The ratios of MGA/MTLs and P/M were applied to reveal the impact of NOx on SOA formation and the aging degree of SOA, respectively. The MGA/MTLs ratios were comparable for the three cities, but much higher than the background value of this region as expected. The P/M ratios suggest that the aging degree of SOA in the YRD region was generally low, but the winter SOA were fresher than the summer SOA. Our research helps to understand pollution characteristics of SOA tracers in the urban agglomeration.

Corrigendum to "Pulsed Radiofrequency of the Trigeminal Ganglion for Treating Postherpetic Neuralgia of the Ophthalmic Branch".

[This corrects the article DOI: 10.1155/2021/6638392.].