[Multi Spectral Detection of Ethanol Content in Gasoline Based on SiPLS Feature Extraction and Information Fusion].

The ethanol content in ethanol gasoline was detected with ultraviolet/visible(UV/vis) and near-infrared (NIR) spectroscopy while information fusion technology and synergy interval PLS(SiPLS) algorithm were used as the feature extraction method with the establishment of partial least squares(PLS) regression model. Using the information fusion theory, UV/vis and NIR spectra were used for data fusion, the data level fusion (Low level data fusion, LLDF) and feature level fusion(Mid-level data fusion, MLDF) model were established. The results were compared with the single source modelwith low level data fusion before vector normalization(LLDF-VN1) selected for the optimal model. Finally, the optimal model was tested using the spectral data collected from the samples of high ethanol content and commercial gasoline. The results showed that both UV/vis and NIR can be used to detect and provide good prediction results, whereas direct fusion of the UV/vis and NIR spectral data provided the best results in the regression model based on the calibration set, with the highest correlation coefficient rc, the smallest Biasc and RMSECV values, as 0.999 9, 0.125 8 and 0.000 6, respectively. And the prediction effect of the model of LLDF-VN1(low level data fusion before vector normalization) was the best, r(p)=0.999 1，Bias(p)=0.352 7，RMSEP=-0.073 8. In the verification of the optimal model (LLDF-VN1) by the self distribution solution, rp=0.999 7, Bias(p)=0.102 2, RMSEP=0.329 1; and that for gasoline sold on market, r(p)=0.990 1, RMSEP=0.675 1, Bias(p)=0.892 7, respectively. It showed that the data level fusion based on UV/vis and NIR spectral information could be used to detect the content of ethanol in ethanol-gasoline quickly and accurately, achieving a wide range of ethanol concentration detection, which laid a foundation for further realization of the rapid detection of substances in the blended fuel oil.

High Expression and Purification of Recombinant Human Serum Albumin from Pichia pastoris.

Recombinant human serum albumin (rHSA) was produced in methylotrophic yeast Pichia pastoris. By optimization of expression, about 150 mg/L of rHSA was obtained from broth of Pichia pastoris GS115/HAS (his+Mut(S)) supernatant. The rHSA was isolated and purified by hollow-fiber ultrafiltration, Phenyl-Sepharose hydrophobic chromatography and antibody-immunoadsorbent chromatography. Finally, rHSA was purified to electrophoretic purity.