RESUMO
In order to study the application of antibodies against recombinant proteins for detecting Borna disease virus (BDV) phosphoprotein (p24) and nucleoprotein (p40) (BDVp24/p40) on paraffin sections by immunohistochemistry. The purified fusion p24 and p40 proteins were used for the preparation of polyclonal and monoclonal antip24 and anti40 antibodies, which were confirmed by ELISA and western blotting. Paraffin sections were made from BDVinfected SpragueDawley (SD) rats (n=20), PBSinjected SD rats (n=20), normal SD rats (n=20) and normal C57 mice (n=20). Immunohistochemical staining was performed according to the EnVision™ twostep protocol. Heatmediated antigen retrieval was performed using the retrieval buffer sodium citrate (1 mM; pH 6.0). All the antibodies against recombinant proteins exhibited good sensitivity and specificity. There were significant differences between the BDVinfected group and the BDVuninfected group for poly and monoclonal antip24 and p40 antibodies. These antibodies against recombinant proteins may be used effectively to detect BDV p24 and p40 in paraffin sections.
Assuntos
Anticorpos Antivirais/imunologia , Vírus da Doença de Borna/imunologia , Imuno-Histoquímica , Nucleoproteínas/imunologia , Fosfoproteínas/imunologia , Proteínas Virais/imunologia , Animais , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos/imunologia , Antígenos Virais/imunologia , Doença de Borna/imunologia , Doença de Borna/virologia , Feminino , Humanos , Imuno-Histoquímica/métodos , Masculino , Coelhos , Ratos , Proteínas Recombinantes/imunologiaRESUMO
For classical Hamiltonian systems, the adiabatic condition may fail at some critical points. However, the breakdown of the adiabatic condition does not always cause the adiabatic evolution to be destroyed. In this paper, we suggest a supplemental condition of the adiabatic evolution for the fixed points of classical Hamiltonian systems when the adiabatic condition breaks down at the critical points. As an example, we investigate the adiabatic evolution of the fixed points of a classical Hamiltonian system which has a number of applications.