Removal of boron by a modified resin in fixed bed column: Breakthrough curve analysis using dynamic adsorption models and artificial neural network model.

Continuous removal of toxic element boron from aqueous solution was investigated with new phenolic hydroxyl modified resin (T-resin) using a fixed bed column reactor operated under various flow rates, bed height and influent concentrations. The breakthrough time, exhaustion time and uptake capacity of the column bed increased with increasing column bed height, whereas decreased with increasing influent flow rate. The breakthrough time and exhaustion time decreased, but uptake capacity increased with increasing influent concentration, and actual uptake capacity was obtained as 6.52 mg/g at a concentration of 7.64 mg/L. The three conventional models of bed depth service time (BDST), Thomas and Yoon-Nelson were used to appropriately predict the whole breakthrough behavior of the column and to estimate the characteristic model parameters for boron removal. However, artificial neural network (ANN) model was more accurate than the conventional models with the least relative error and the highest correlation coefficients. By the relative importance of the operational parameters obtained from ANN model, the sequence is as follows: total effluent time > initial concentration > flow rate > column height. The adsorption capacity of boron was changed between 5.24 and 1.74 mg/g during the five time regeneration. From the life factor calculation, it is suggested that the column bed could avoid the breakthrough time of t = 0 for 6.8 cycles, whereas, the uptake capacity would be zero after 7.8 cycles.

Controllable assembly of a novel cationic gemini surfactant containing a naphthalene and amide spacer with ß-cyclodextrin.

A novel cationic gemini surfactant (C12NDDA) with a spacer containing naphthalene and amides was successfully synthesized. The assembly of C12NDDA with ß-cyclodextrin (ß-CD) was investigated using various techniques including transmission electron microscopy, proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy. Tuning the C12NDDA concentration and the C12NDDA/ß-CD molar ratio allowed the production of different assembled aggregate morphologies such as micelles, vesicles, nanowires, nanorods, and hydrogels. Investigation of the inclusion mechanisms of C12NDDA and ß-CD by 1H NMR revealed that hydrophobic interactions, hydrogen bonding, π-π stacking, and electrostatic forces play key roles in the assembly process. The antimicrobial activities of the C12NDDA/xß-CD (x = 0-4) inclusion complexes were tested against Gram-negative bacteria (Escherichia coli and Salmonella) and Gram-positive bacteria (Staphylococcus aureus and Streptococcus), and very low minimum inhibitory concentrations of 0.078-0.31 µg mL-1 were observed. Thus, this newly synthesized gemini surfactant and its inclusion complexes exhibit potential as superior broad-spectrum disinfectants for various biomedical and biotechnological applications.

Transplanting GABAergic Neurons Differentiated from Neural Stem Cells into Hippocampus Inhibits Seizures and Epileptiform Discharges in Pilocarpine-Induced Temporal Lobe Epilepsy Model.

OBJECTIVE: This study aimed to explore whether intrahippocampal transplantation of GABAergic neurons generated in vitro ameliorated seizures and epileptiform discharges via increasing γ-aminobutyric acid (GABA)-associated inhibition mediated by the addition of new GABAergic neurons. METHODS: Neural stem cells (NSCs) isolated from newborn rats were induced and differentiated into GABAergic neurons. A total of 36 Pilocarpine-induced pharmacoresistant epileptic rats were divided into 3 groups: PBS (phosphate-buffered saline) group, NSCs group, and GABAergic neurons group (GABA group), with an additional 10 normal rats used (normal rat control group). The effects of grafting on spontaneous recurrent seizures (SRS) were examined and hippocampal GABA content was measured after grafting. RESULTS: In the GABA group, the frequency of electroencephalography decreased significantly compared with the PBS group (P < 0.001), but there was no significant difference between the GABA group and NSCs group. Compared with the PBS group, the overall frequency and duration of SRS significantly decreased in the transplantation group, especially in the GABA group (P < 0.01). The number of GABAergic neurons was highest in the GABA group compared with the other groups (P < 0.001). Furthermore, hippocampal GABA concentrations significantly increased in the GABA group. CONCLUSIONS: We show that GABAergic neurons generated in vitro from NSCs and grafted into the hippocampi of chronically epileptic rats can significantly reduce the frequency of electroencephalography and frequency and duration of SRS via increasing GABA-associated inhibition mediated by the addition of new GABAergic neurons.