Trace analysis of persistent toxic substances in the main stream of Jiangsu section of the Yangtze River, China.

BACKGROUND, AIM, AND SCOPE: The Jiangsu section of the Yangtze River is the downstream of the whole river, serving as an important drinking water source. Persistent toxic substances (PTS), from the industries such as automobile, textile, chemical, and electronic production, are not listed in the National Standard yet and not monitored and controlled. However, pollution of PTS can threaten the environment and human health. In order to understand the pollution status of the PTS contamination and recommend future rationalization of countermeasure, the PTS including organochlorine pesticides (OCPs), polyaromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs), and phthalates (PAEs) were investigated. MATERIALS AND METHODS: Samples were collected at 15 sites from five main stream sections of the Yangtze River in Jiangsu Province. PTS were extracted using solid phase extract method. The analysis of OCPs, PCBs was performed using GC/ECD and PAHs, PAEs was performed by GC/MS. RESULTS AND DISCUSSIONS: The method detection limits are low and the recoveries are from 58.1% to 110.3%. The total concentrations of OCPs, PCBs, PAHs, and PAEs were 0.27∼2.15 ng l(-1), <0.21∼44.4 ng l(-1), 0.012∼3.576 µg l(-1) and 0.178∼1.474 µg l(-1), respectively. γ-HCH, PCB28, PCB101, phenanthrene, anthracene, di-n-butyl phthalate, diethyl phthalate, and di-(2-ethylhexyl) phthalate are the major detected PTS. The concentration of most of the PTS was below the environmental quality standards for surface water of China. CONCLUSIONS: The pollution status of OCPs, PCBs, PAHs, and PAEs were analyzed. PTS contamination levels of the five main stream sections were relatively low.

[Effect of Myostatin gene vaccine on immunized mice].

AIM: To characterize the immunological function of Myostatin gene vaccine and observe the effect of Myostatin gene vaccine on the immunized animal. METHODS: The mice were immunized with Myostatin gene vaccine. We characterized the antibody titer in the immunized mice serum by the ELISA. The anti-serum was detected by auto-biochemistry analysis software. Meanwhile, the effect anti-serum was detected by auto-biochemistry analysis software. Meanwhile, the effect of Myostatin gene vaccine on skeletal-muscle of the immunized mice was analyzed by HE stain. The cross section area of muscle fiber in immunized mice was analyzed by the Scion Image 4.02 software. RESULTS: Myostatin gene vaccine could induce the anti-serum against Myostatin in immunized mice. Compared with that in the control group, the mean weight in the pVAC-TT-Ms immunized mice group increased with 9.8%. The quadriceps muscle, gastrocnemius muscle and pectoralis magor in immunized mice increased with 24.1%, 10.9% and 20.3%, respectively. CONCLUSION: Myostatin gene vaccine induced the specific antibody against Myostatin, and made the muscle strong.