[Effects of matrix metalloproteinases inhibitor GM6001 on choroidal neovascularization].

OBJECTIVE: To explore the efficiency of GM6001 for the inhibition of choroidal neovascularization. METHODS: Experimental study. Twenty-four Brown Norvy (BN) rats after photocoagulation were randomly divided into 3 groups as GM6001 group, DMSO group and CONT group. GM6001 (0.2 ml of 0.1% suspension) was injected retrobulbar for GM6001 group and 0.2 ml DMSO was injected for DMSO group on days 1, 3, 6, 9, and 12 after photocoagulation. No injection was performed in the CONT group. Fundus fluorescence angiography, histopathology, immunohistochemistry and quantitative analysis of choroidal neovascularization (CNV) were performed 3 weeks after photocoagulation. One-way ANOVA was used in conjunction with SNK-t test to assess statistical significance within groups. RESULTS: The fluorescein leakage appeared in all three groups; but fluorescein leakage of GM6001 group (74.56 ± 2.33) was less than that of DMSO group (119.57 ± 1.15)and CONT group (122.36 ± 2.38) (F = 403.23, P = 0.001; LSD-t test, all P value < 0.01), whereas fluorescein leakage of DMSO group was similar to that in the CONT group. The retinal and choroidal capillaries in the CONT group were damaged and disordered; a great deal of CNV and migration and proliferation of retinal pigment epithelium cells, fibrocytes and collagen fibers were discovered. Pathological changes in DMSO group were similar to those in the CONT group. There were a small quantity of retinal pigment epithelium cells, fibrocytes and CNV in GM6001 group. Although the immunohistochemical staining for CD105 displayed positive results in all three groups, positive staining of GM6001 group (19.85 ± 1.59) was significantly less than that of the CONT group (38.02 ± 2.57) and DMSO group (39.02 ± 3.12) (F = 55.57, P = 0.001; LSD-t test, all P value < 0.01). Positive staining of CD105 in the CONT group was similar to that of DMSO group (P > 0.05). The size of CNV in GM6001 group (15.35 ± 0.77) was significantly less than that of CONT group (28.38 ± 1.60) and DMSO group (28.74 ± 1.19) (F = 114.85, P = 0.001; LSD-t test, all P value < 0.01). There was no statistical difference for the size of CNV between CONT group and DMSO group (P > 0.05). CONCLUSION: GM6001 effectively inhibits CVS induced by krypton laser photocoagulation.

[Gene expression patterns of choroidal neovascularization induced by krypton laser photocoagulation].

OBJECTIVE: To explore the gene expression patterns of choroidal neovascularization with gene chip technology. METHODS: Total RNA was isolated from normal choroid and choroid tissues with neovascularization induced by krypton laser photocoagulation in Brown Norway rat. The mRNA was purified and was reversely transcribed to cDNA to prepare for hybridization probes. In choroidal neovascularization, the differentially expressed genes were assayed with gene chips which contained 5705 rat genes. RESULTS: There were 46 genes expressed differentially in choroidal neovascularization. Of these, the expression of 3 genes was upregulated and 43 genes downregulated. These genes were involved in the function of transport, signal, metabolism, development and differentiation of cell adhesion. CONCLUSION: Numerous genes are involved in the development of choroidal neovascularization induced by krypton laser photocoagulation.

Separation of compounds interacting with liposome membrane in combined prescription of traditional Chinese medicines with immobilized liposome chromatography.

Immobilized liposome chromatography (ILC), the stationary phase of which has been regarded as a mimic biomembranes system was used to separate and analyze compounds interacting with liposome membrane in Danggui Buxue decoction, a combined prescription of traditional Chinese medicines (CPTCMs), and its compositions Radix Astragli and Radix Angelica Sinensis. More than 10 main peaks in the extract of Danggui Buxue decoction were resolved on the ILC column, suggesting that more than 10 components in the prescription have significant retention on ILC column. Ligustilide, astragaloside IV and formononetin, three main bioactive ingredients in Danggui Buxue decoction, were found to have relatively significant, while ferulic acid, another bioactive ingredient in the prescription, relatively weak retention on ILC column. Effects of the eluent pH and amount of immobilized phosphatidylcholine (PC) on separation of interactional compounds in the extract of Danggui Buxue decoction were also investigated. It was found that these two factors strongly affected the retention of some interactional compounds. In addition, the fractions partitioned with different solvents from water extract of this combined prescription were evaluated with this ILC column system.

[Application of genetic algorithm in optimization of mobile phase composition in high performance liquid chromatography].

Referring to traditional optimal methods, a method for the optimization of isocratic elution mobile phase composition in high performance liquid chromatography has been developed. In this method, the genetic algorithm based on line-crossover and plane-mutation is used. The principle of genetic algorithm and the process of optimization of mobile phase composition in reversed-phase ion-pair high performance liquid chromatography using genetic algorithm are introduced in details. With the concentrations of acetonitrile and ion-pair reagent sodium octane sulfonate chosen as the optimal parameters, the optimum was obtained by three times of optimization procedures. The mean relative error between the predicted and experimental values was 0.75% at the optimum and the optimization results were satisfactory.