RESUMO
The present letter to the editor is related to the study titled 'Angiotensin-converting enzyme 2 improves liver fibrosis in mice by regulating autophagy of hepatic stellate cells'. Angiotensin-converting enzyme 2 can alleviate liver fibrosis by regulating autophagy of hepatic stellate cells and affecting the renin-angiotensin system.
Assuntos
Peptidil Dipeptidase A , Sistema Renina-Angiotensina , Animais , Camundongos , Angiotensina II/metabolismo , Enzima de Conversão de Angiotensina 2 , Fibrose , Células Estreladas do Fígado/metabolismo , Cirrose Hepática , Peptidil Dipeptidase A/metabolismoRESUMO
To evaluate the effect of glucose and fructose on the glycation of ß-lactoglobulin (ß-Lg) in intermediate-moisture food (IMF), model systems consisting of ß-Lg, glucose/fructose/sorbitol, glycerol, and water were established. All systems were stored at 25 and 35 °C for 2 months. The progress of the Maillard reaction and the mass change of ß-Lg were investigated by the browning assay and gel electrophoresis, respectively. Meanwhile, liquid chromatography-mass spectrometry (LC-MS) and data-independent acquisition LC-MS (LC-MS(E)) were used to monitor the glycation extent and the glycated sites of ß-Lg. The results indicated that glucose had a higher reaction activity of glycation than fructose, but both sugars had similar preference on the glycation site for ß-Lg. The ranking order from high to low for the 9 detected glycated sites was L 1, K 91 > K 47 > K 70, K 77, K 83, K 100 > K 75 > K 135 for both sugars.
