Evaluation of the Clinical Efficacy of the Classic Prescription "Baihe Dihuang Decoction" Based on Meta-Analysis.

Purpose: To explore the clinical application of Baihe Dihuang Decoction. To provide certain data support and theoretical basis for the clinical application of Baihe Dihuang Decoction in the future. Methods: With "Baihe Rehmannia Tang" as the search term, the search was carried out on CNKI, VIP, Wanfang, PubMed and other databases. The statistical analysis of Baihe Dihuang decoction for treating diseases was obtained. Meta-analysis of the data was performed using RevMan 5.3 software to analyze the main therapeutic indicators of the disease. Results: According to the 83 valid literature that can be found, it is shown that 17 are used for the treatment of depression, 14 are used for the treatment of menopausal syndrome, 24 are used for the treatment of insomnia, and 28 are used for the treatment of other diseases. Conclusion: In the treatment of depression, menopausal syndrome, and insomnia combined with Baihe Dihuang Decoction can have a better therapeutic effect and diminish the incidence of adverse reactions. It provides a theoretical basis for the study and experimental study of its active components.

[Powder modificationfor improving content uniformity of Ziyin Yiwei Capsules].

Based on the defects in powder properties of the contents of Ziyin Yiwei Capsules, this study screened out the main medicinal slice powders causing the poor powdery properties, and introduced the powder modification process to improve the powdery properties of these slice powders, the pharmaceutical properties of the capsule contents, and the content uniformity of Ziyin Yiwei Capsules, so as to provide a demonstration for the application of powder modification technology to the preparation of Chinese medicinal solid preparations. Through the investigation on the powder properties of the contents of Ziyin Yiwei Capsules, it was clarified that the pulverized particle size of the capsule contents had a good correlation with the pulverization time. According to the measurement results of the powder fluidity and wettability, the quality defects of the capsule contents were caused by the fine powders of Taraxaci Herba and Lungwortlike Herba. "Core-shell" composite particles were prepared from medicinal excipients magnesium stearate and fine powders of Taraxaci Herba and Lungwortlike Herba slices after ultra-fine pulverization to improve the powder properties of the problematic fine powders. Powder characterization data including fluidity and wettability were measured, followed by scanning electron microscopy(SEM) and infrared ray(IR) detection. It was determined that the optimal dosage of magnesium stearate was 2%, and the compositing time was 3 min. The composite particles were then used as content components of the Ziyin Yiwei Capsules. The powder characteristics between the original capsule and the modified composite capsule including the particle size, fluidity, wettability, uniformity of bulk density, and uniformity of chromatism as well as the content uniformity and in vitro dissolution were compared. The results showed that the powder characteristics and content uniformity of the prepared composite capsule were significantly improved, while the material basis of the preparation was not changed before and after modification. The preparation process was proved to be stable and feasible. The powder modification technology solved the pharmaceutical defects that were easy to appear in the preparation of traditional capsules, which has provided experimental evidence for the use of powder modification technology for improving the quality of Chinese medicinal solid preparations and promoting the secondary development and upgrading of traditional Chinese medicinal dosage forms such as capsules.

The effect of the degree of substitution on the solubility of cellulose acetoacetates in water: A molecular dynamics simulation and density functional theory study.

The effect of the degree of substitution (DS) on the aqueous solubility of cellulose acetoacetates (CAA) was investigated by molecular dynamics simulations and density functional theory calculations. Using average non-covalent interaction and the electrostatic potential analyses done on cellobiose as the model, it was showed both polar and non-polar areas of the system increased as the more hydroxyls were replaced by acetoacetate groups. Analyses of the solvation free energies of a celludecose (glucan containing 10 monosaccharide sugar units) at constant pressure and temperature showed the polar solvation free energies and the number of decose-water hydrogen bonds increased as DS was varied from 0.3 to 0.8, which contributes to higher solubility in water. When the DS of CAA increased from 0.8 to 1.5, it became insoluble again because the plateaued increase in solvation free energy could no longer compensate for the decreasing CAA-water hydrogen bonding interactions. The growing van der Waals interactions among CAA molecules as the molecule grows bigger with each attached AA group also contributes to the decreasing water solubility.

Preparation and characterization of carboxymethylated cotton fabrics as hemostatic wound dressing.

Uncontrolled hemorrhage is a large cause of death in the global scope, thus leading to an urgent demand to develop efficient hemostatic materials. In this study, a series of modified cotton fabrics (MCFs) with different carboxymethyl group contents were prepared from cotton fabric (CF) by a carboxymethylation process to choose the appropriate one with the best hemostatic performance. The carboxymethyl group contents of MCFs rose up as the dosages of ClCH2COOH increased. The crystallinity of CF decreased after carboxymethylation, and MCFs can dissolve slightly with the phenomenon that there were vague boundaries between fibers after being treated with water. Furthermore, the MCF with the carboxymethyl group content at 0.77 mmol/g (MCF-0.77) could absorb the blood quickly, achieve dense distribution of blood cells and have high viscosity of leaching liquor. In addition, the MCF-0.77 with good biocompatibility accelerated the hemostasis time to 46.6 ± 8.4 s compared with the CF (88.8 ± 31.5 s) in a rat model of liver injury. In summary, the prepared MCF-0.77 is a potential hemostatic wound dressing for clinical use since every second counts for pre-hospital care.

Aggregation behaviors of thermo-responsive methylcellulose in water: A molecular dynamics simulation study.

The aggregation behaviors of methylcellulose (MC) in aqueous solution were investigated using all-atom molecular dynamic simulations (MD). The interactions between MC chains and water molecules at different temperatures were investigated by a series of MD analyses, such as the solvent accessible surface area, number of hydrogen bonds, radial distribution functions and the interaction energies. Constant temperature simulations and heating simulations of MC aqueous solution were carried out in this work. In the simulations at three constant temperatures (25 °C, 50 °C and 75 °C), the aggregation behaviors of MC chains were affected by the temperature. In the heating simulation (25 °C âˆ¼ 75 °C), temperature increases were accompanied by decreases in interactions between MC and water molecules, and by increases in interactions between MC chains, which led to the aggregation of MC chains. The degree of aggregation of MC chains increased with the rise of temperature.

Real-time monitoring of multicomponent reactive dye adsorption on cotton fabrics by Raman spectroscopy.

Accurate real-time determination of each dye in combination dyeing is critical to the control of dyeing process, which plays an important role in upgrading the dyeing techniques of textile. In this work, Raman spectroscopy was applied to dyeing baths containing multiple dye species of varying structures to quantitatively monitor the dyeing process of each individual dye. Quantitative models were successfully established by partial least squares (PLS) for all combinations of the nine commonly used reactive dyes studied. The correlation coefficients were greater than 0.99, the root mean squared errors of calibration (RMSEC) were less than 0.2650 and the root mean squared errors of prediction (RMSEP) were less than 0.1340, even for the three-component mixture of Reactive Red 239 (RR239), Reactive Yellow 176 (RY176) and Reactive Blue 194 (RB194), which are similar in structures. The model was shown to be valid in the presence of added electrolytes (sodium sulfates). Real-time adsorption monitoring based on the model revealed that the dyes interacted with one another and competed for active sites. The adsorption kinetics obtained by Raman analysis shed light on dye compatibility and could be used to guide the design of dyeing recipe and dyeing process for optimum color reproduction. In addition, in situ monitoring by Raman spectroscopy maybe integrated with real-time on line control of dyeing parameters for fully automated production of dyed fabrics.

The fabrication of polylactide/cellulose nanocomposites with enhanced crystallization and mechanical properties.

Polylactide/cellulose nanocomposites were fabricated by blending of commercial polylactide (PLA) and modified cellulose nanocrystals (CNCs). Modified CNCs were prepared via the in situ polymerization of CNCs and L-lactic acid (CNCs-PLLA) or D-lactic acid (CNCs-PDLA). The actual occurrence of chemical bond between CNCs and PLA segment was confirmed by Fourier transform infrared, nuclear magnetic resonance, X-ray diffraction and solubility tests. Differential scanning calorimetry and X-ray diffraction characterization indicated that CNCs-PDLA better improved the crystallization ability of PLA matrix compared with CNCs-PLLA. Furthermore, compared with the neat PLA (60.0 MPa), the tensile strength of resulting nanocomposites showed an enhancement of up to 36% (81.65 MPa). And the nanocomposites with CNCs-PDLA exhibited both high crystallinity and improved mechanical properties.

Regenerated cellulose-dispersed polystyrene composites enabled via Pickering emulsion polymerization.

Cellulose composites are an important class of polymeric composites due to their renewable, biodegradable feedstock, and tend to have exceptional properties due to peculiar increase of the matrix-filler interface. In this work, a new breed of colloids enabled by regenerated cellulose (RC) was used to prepare polystyrene/cellulose composites. Specifically, an oil-in-water Pickering emulsion of styrene stabilized by RC was prepared, followed by polymerization of styrene, allowing us to obtain a uniformly dispersed PS/RC composite. When the RC concentration was above 0.8 wt%, a network of RC spontaneously formed in the composite, as evidenced by rheological testing. Furthermore, the addition of RC to polystyrene improved the composite's thermal stability and tensile mechanical properties while minimally impacting average visible light transmission (with a decrease in average transmission by about 1-5%). Regenerated cellulose is a promising nanofiller for polymeric composites due to its environment friendly and cost effective nature, and could be used for preparation of other novel RC-reinforced composites.

Bio-based polymer colorants from nonaqueous reactive dyeing of regenerated cellulose for plastics and textiles.

A general way to prepare bio-based polymer colorant by reactive dyeing of regenerated cellulose (RC) in dimethyl sulfoxide (DMSO) was established. Up to 40% higher dye utilization was achieved compared with aqueous reactive dyeing of RC for two reasons. 1. Lower liquor ratio of 1:9 could be applied for the concentrated RC suspensions in DMSO was less viscous. 2. Dye loss from solvolysis was turned off in DMSO. The method was generally applicable to RCs made from wood pulp, waste cotton, and bamboo pulp. The dyeing process has minimum influence on the morphological, rheological, and thermal properties of RC. The colored RCs thus prepared were used to prepare colored polymer composites via a Pickering emulsion approach, and to color cotton, cotton blend, and viscose fabrics via a printing approach. Therefore, it holds great potential as renewable and biodegradable pigments for making colorful composites, ink, textile dyeing and finishing.

Diosgenin inhibited the expression of TAZ in hepatocellular carcinoma.

Emerging evidence has supported that TAZ (transcriptional co-activator with PDZ binding motif), one transcription co-activator in Hippo signaling pathway, plays an oncogenic role in liver carcinogenesis. Targeting TAZ could be a potential therapeutic approach for liver cancer patients. In the current study, we aim to determine whether diosgenin could be an inhibitor of TAZ in liver cancer cells. We found that diosgenin inhibited the expression of TAZ in liver cancer cells. Moreover, we found that diosgenin inhibited cell growth, induced apoptosis, suppressed cell migration and invasion in part via inhibition of TAZ in liver cancer cells. Our study provides the evidence to support that diosgenin could be a potential agent for treating human liver cancer.

Benign Fabrication of Fully Stereocomplex Polylactide with High Molecular Weights via a Thermally Induced Technique.

A reproducible and environmentally friendly method for the preparation of high molecular-weight stereocomplex polylactide (HMW SCPLA) is achieved. Poly(l-lactide) and poly(d-lactide) were simply dissolved in an environmentally friendly solvent, dibasic ester (DBE), at 110 °C. Then, the two solutions were mixed and cooled to room temperature, and the HMW SCPLA spontaneously precipitated in the form of fine powder consequently. The presence of the DBE reduced the reaction temperature and improved the molecular mobility of the polymers; thus, the degradation problems and the molecular diffusion issue in the process of the formation of the stereocomplex could be overcome. The relationship among the concentration of the mixture, degree of stereo-complexation, and thermal properties of SCPLA powders was also established. Moreover, porous membrane and film SCPLA material with good thermal properties were also obtained using this thermally induced technique. This method could be a good approach to expand the SCPLA applications.

(-)-Epigallocatechin-3-gallate attenuates myocardial injury induced by ischemia/reperfusion in diabetic rats and in H9c2 cells under hyperglycemic conditions.

(-)-Epigallocatechin gallate (EGCG) exerts multiple beneficial effects on cardiovascular performance. In this study, we aimed to examine the effects of EGCG on diabetic cardiomyopathy during myocardial ischemia/reperfusion (I/R) injury. EGCG (100 mg/kg/day) was administered at week 6 for 2 weeks to diabetic rats following the induction of type 1 diabetes by streptozotocin (STZ). At the end of week 8, the animals were subjected to myocardial I/R injury. The EGCG-elicited structural and functional effects were analyzed. Additionally, EGCG (20 µM) was administered for 24 h to cultured cardiac H9c2 cells under hyperglycemic conditions (30 mM glucose) prior to hypoxia/reoxygenation (H/R) challenge, and its effects on oxidative stress were compared to H9c2 cells transfecteed with silent information regulator 1 (SIRT1) small interfering RNA (siRNA). In rats with STZ-induced diabetes, EGCG treatment ameliorated post-ischemic cardiac dysfunction, decreased the myocardial infarct size, apoptosis and cardiac fibrosis, and reduced the elevated lactate dehydrogenase (LDH) and malonaldehyde (MDA) levels, and attenuated oxidative stress. Furthermore, EGCG significantly reduced H/R injury in cardiac H9c2 cells exposed to high glucose as evidenced by reduced apoptotic cell death and oxidative stress. The protein expression levels of SIRT1 and manganese superoxide dismutase (MnSOD) were reduced in the diabetic rats and the H9c2 cells under hyperglycemic conditions, compared with the control rats following I/R injury and H9c2 cells under normal glucose conditions. EGCG pre-treatment significantly upregulated the levels of htese proteins in vitro and in vivo. However, treatment with EX527 and SIRT1 siRNA blocked the EGCG-mediated cardioprotective effects. Taken together, our data indicate that SIRT1 plays a critical role in the EGCG-mediated amelioration of I/R injury in diabetic rats, which suggests that EGCG may be a promising dietary supplement for the prevention of diabetic cardiomyopathy.

Berberine Induced Apoptosis of Human Osteosarcoma Cells by Inhibiting Phosphoinositide 3 Kinase/Protein Kinase B (PI3K/Akt) Signal Pathway Activation.

BACKGROUND: Osteosarcoma is a malignant tumor with high mortality but effective therapy has not yet been developed. Berberine, an isoquinoline alkaloid component in several Chinese herbs including Huanglian, has been shown to induce growth inhibition and the apoptosis of certain cancer cells. The aim of this study was to determine the role of berberine on human osteosarcoma cell lines U2OS and its potential mechanism. METHODS: The proliferation effect of U20S was exanimed by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di- phenytetrazoliumromide (MTT) and the percentage of apoptotic cells were determined by flow cytometric analysis. The expression of PI3K, p-Akt, Bax, Bcl-2, cleavage-PARP and Caspase3 were detected by Western blott. RESULTS: Berberine treatment caused dose-dependent inhibiting proliferation and inducing apoptosis of U20S cell. Mechanistically, berberine inhibits PI3K/AKT activation that, in turn, results in up-regulating the expression of Bax, and PARP and down-regulating the expression of Bcl-2 and caspase3. In all, berberine can suppress the proliferation and induce the apoptosis of U2OS cell through inhibiting the PI3K/Akt signaling pathway activation. CONCLUSION: Berberine can suppress the proliferation and induce the apoptosis of U2OS cell through inhibiting the PI3K/Akt signaling pathway activation.

Elevated microRNA-25 inhibits cell apoptosis in lung cancer by targeting RGS3.

The non-small-cell lung cancer (NSCLC) is the most common type of lung cancer that affects the human health. But, the underlying mechanisms and effective therapy are still absent. MicroRNAs (miRNAs) are small RNAs that specifically bind to the 3' untranslated region (3'UTR) of its target and regulate the protein level of the target at post-transcriptional level. A lot of miRNAs had been found abnormally regulated in the NSCLC patients, and understanding their specific roles in the pathogenesis of NSCLC will help us to develop novel therapeutic approaches. Here, we reported that miR-25 is dramatically upregulated in NSCLC tissues and negatively correlated with RGS3 protein. A conserved binding sequence in the 3'UTR of RGS3 gene to miR-25 was identified, and overexpression of miR-25 induces the RGS3 inhibition. Importantly, suppression of miR-25 facilitates the cell apoptosis and retards the cell proliferation in A549 and H520 cell lines. Our data provide a novel miR-25/RGS3 signal in the development of lung cancer.

LY294002 prevents lipopolysaccharide­induced hepatitis in a murine model by suppressing IκB phosphorylation.

Although fulminant hepatitis represents a ubiquitous human health problem, there is a lack of effective therapeutic strategies that have few side­effects and the precise mechanisms underlying fulminant hepatitis are not fully understood. Phosphoinositide 3­kinase (PI3K) is a pivotal kinase known to regulate inflammatory responses in hepatic diseases. Although previous research indicates that PI3K is involved in cardiac diseases, including myocardial infarction, it currently remains unclear whether the inhibition of PI3K is essential for ameliorating the severity of lipopolysaccharide (LPS)­induced hepatitis. The aim of the present study was to investigate whether pharmacological blockade of PI3K ameliorates the development of LPS­induced murine acute hepatic injury. A murine model of LPS­induced acute hepatic injury was used to investigate the therapeutic effect of the pan­PI3K inhibitor, LY294002 on murine fulminant hepatitis and to investigate potential underlying mechanisms. The current report presents the in vivo role of LY294002 in protecting the mice from fulminant hepatitis. LY294002 was observed to exert significant protective effects on the liver by reducing the activities of alanine aminotransferase and aspartate aminotransferase, as well as by improving the histological architecture of the liver. In LPS­induced hepatitis, treatment with LY294002 clearly inhibited intrahepatic synthesis of various disease­relevant proinflammatory cytokines, including tumor necrosis factor­α, interleukin (IL)­6, IL­1ß and interferon­Î³. Furthermore, LY294002 was observed to significantly inhibit IκB phosphorylation in LPS­injured mouse liver samples. Therefore, LY294002 may protect the liver from LPS­induced injury by inhibition of the IκB­nuclear factor κ­light­chain­enhancer of activated B cell dependent signaling pathway. Thus, the current report provides evidence that LY294002 exerts potent effects against LPS­induced hepatic injury, indicating its potential therapeutic value for the treatment of acute hepatitis.

Biomarker expression in lung of rabbit with pulmonary fibrosis induced by ammonium perchlorate.

Ammonium perchlorate (AP), an oxidizer, has been used in solid propellants. Although AP exposure has been suspected as a risk factor for the development of pulmonary fibrosis, data are still inconclusive. To evaluate the pulmonary toxicity and the potential pulmonary fibrosis caused by occupational exposure to this compound, 25 male rabbits were randomly allocated into five groups to receive AP or bleomycin or saline by intratracheal injection. All rabbits were sacrificed and total RNA from the lungs was extracted. Expressions of types I and III collagens, transforming growth factor-ß(1) (TGF-ß(1)) and tumour necrosis factor-α (TNF-α) messenger RNA (mRNA) were measured by reverse transcription-polymerase chain reaction (RT-PCR). The expressions of type I and III collagen mRNA in low, moderate and high dose AP groups were significantly higher (p < 0.01 or p < 0.05) than that in the saline group. There was also a significant increased level of TGF-ß(1) and TNF-α mRNA in the three AP groups compared with saline control group (p < 0.01 or p < 0.05). These results reveal that AP can increase gene expressions of types I, III collagens, TGF-ß(1) and TNF-α in lung of rabbits exposed to AP. The overexpression of these biomarkers were considered as effective indicator linking to the development of pulmonary fibrosis and finally demonstrated that AP has potential to induce pulmonary fibrosis.